Click the links in the Agree and Disagree columns to view validation images, notes, and accompanying publications for a listed reagent. View reagents based on your specific needs by filtering on the relevant criteria (protein biomarker, tissue preservation, etc.).
Additional information on fluorescent probes tested by the IBEX Imaging Community can be found here. Details on reagent validation can be found here.
Notes:
- Loading the filterable reagents table takes some time but is worth the wait.
- You can readily download the underlying comma separated value file for the reagent resources (“Save Link As…”) or download the whole knowledge-base as a zip file and go to the
datadirectory that contains all csv files.
| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q9BYF1 | Primary Antibody | ACE2 | Human | Goat | IgG | Polyclonal | R&D Systems | AF933 (Unconjugated) | AF555 | AB_2892761 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9GZX7 | Primary Antibody | AID | Human | Mouse | IgG1 | 39-2500 | Thermo Fisher Scientific | ZA001 | Unconjugated | AB_2533403 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9GZX7 | Primary Antibody | AID | Human | Rat | IgG2a | mAID-2 | Thermo Fisher Scientific | 14-5959-82 | Unconjugated | AB_10669583 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Novus Biologicals | IC1420S-025 | AF750 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Abcam | ab184675 | AF488 | AB_2832195 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Alpha smooth muscle actin | Canine | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| NA | Primary Antibody | Alpha smooth muscle actin | Canine | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| NA | Primary Antibody | Alpha smooth muscle actin | Rhesus Macaque | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | Novus Biologicals | NBP2-34522IR | DL755 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | Novus Biologicals | NBP2-34522IR | DL755 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P25054 | Secondary Antibody | APC | Human | Mouse | IgG2b | 936809 | R&D Systems | FAB8927R-100UG | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29972 | Primary Antibody | Aquaporin 1 | Human | Rabbit | IgG | EPR11588(B) | Abcam | ab225225 | AF647 | AB_2810992 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P41181 | Primary Antibody | Aquaporin 2 | Human | Goat | IgG | Polyclonal | Novus Biologicals | NBP1-70378 | Unconjugated | AB_11011225 | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41181 | Primary Antibody | Aquaporin 2 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-33472 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05089 | Primary Antibody | Arginase-1 | Mouse | Rabbit | IgG | D4E3M | Cell Signaling Technology | 93668 | Unconjugated | AB_2800207 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 |
| P36575 | Primary Antibody | Arrestin C | Human | Mouse | IgG1 | 7G6 | MilliporeSigma | MABN2636-100UG | Unconjugated | AB_2935804 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P00966 | Primary Antibody | ASS1 | Human | Rabbit | IgG | EPR12398 | Abcam | ab170952 | Unconjugated | AB_2892766 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103289 | Spark Blue 574 | AB_2904275 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 48-0452-82 | eF450 | AB_1548761 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103248 | BV510 | AB_2650679 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103294 | Spark Red 718 | AB_2924441 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 41-0452-80 | eF570 | AB_2573598 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 41-0452-80 | eF570 | AB_2573598 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103251 | BV421 | AB_2562905 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 557683 | AF647 | AB_396793 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 46-0452-82 | PerCP-eF710 | AB_10717389 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Placenta | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103232 | AF700 | AB_493717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 42-0452-82 | eF615 | AB_10852702 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 58-0452-82 | AF532 | AB_11218492 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103294 | Spark Red 718 | AB_2924441 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | No | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 557669 | AF488 | AB_396781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658705 | AF647 | AB_2563279 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658707 | PE | AB_2563281 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658703 | AF488 | AB_2563151 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658707 | PE | AB_2563281 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41182 | Primary Antibody | BCL6 | Human | Mouse | IgG1 | K112-91 | BD Biosciences | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41182 | Primary Antibody | BCL6 | Human | Mouse | IgG1 | GI191E | Thermo Fisher Scientific | 14-9887-82 | Unconjugated | AB_10669166 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41183 | Primary Antibody | BCL6 | Mouse | Mouse | IgG1 | K112-91 | BD Biosciences | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41182 | Primary Antibody | BCL6 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-59786 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41182 | Primary Antibody | BCL6 | Human | Mouse | IgG1 | K112-91 | BD Biosciences | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51491 | Primary Antibody | Blue opsin | Mouse | Rabbit | IgG | Polyclonal | MilliporeSigma | AB5407 | Unconjugated | AB_177457 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P03999 | Primary Antibody | Blue opsin | Human | Rabbit | IgG | Polyclonal | MilliporeSigma | AB5407 | Unconjugated | AB_177457 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| Q9W6A8 | Primary Antibody | Blue opsin | Zebrafish | Rabbit | IgG | Polyclonal | Kerafast | EJH012 | Unconjugated | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P35226 | Primary Antibody | BMI-1 | Human | Mouse | IgG2b | LLBmi1-1 | Novus Biologicals | NBP1-96140AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01106 | Primary Antibody | c-Myc | Human | Mouse | IgG1 | 9E10 | R&D Systems | MAB3696-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01106 | Primary Antibody | c-Myc | Human | Mouse | IgG1 | 9E10 | Novus Biologicals | NB600-302IR | DL755 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| E9QJF8 | Primary Antibody | Calbindin | Zebrafish | Rabbit | IgG | Polyclonal | Swant | CB38a | Unconjugated | AB_10000340 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P05937 | Primary Antibody | Calbindin | Human | Rabbit | IgG | Polyclonal | MilliporeSigma | ABN2192 | Unconjugated | AB_2935805 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P22676 | Primary Antibody | Calretinin | Mouse | Mouse | IgG1 | 6B8.2 | MilliporeSigma | MAB1568 | Unconjugated | AB_94259 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| A0A1A8U8H3 | Primary Antibody | Calretinin | Turquoise Killifish | Rabbit | IgG | Polyclonal | Swant | 7697 | Unconjugated | AB_2721226 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q6PC44 | Primary Antibody | Calretinin | Zebrafish | Rabbit | IgG | Polyclonal | Swant | 7697 | Unconjugated | AB_2721226 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Primary Antibody | Carbonic anhydrase 1 | Zebrafish | Rabbit | IgG | EPR5193 | Abcam | ab108367 | Unconjugated | AB_10863424 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P07711 | Primary Antibody | Cathepsin L | Human | Rat | IgG2a | 204101 | R&D Systems | MAB-9521 | Unconjugated | AB_2087829 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99731 | Primary Antibody | CCL19 | Human | Mouse | IgG1 | OTI2A12 | Novus Biologicals | NBP2-71278AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| O15444 | Primary Antibody | CCL25 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-49199 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P32248 | Primary Antibody | CCR7 | Human | Rabbit | IgG | Polyclonal | LSBio | LS-C210538 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32248 | Primary Antibody | CCR7 | Human | Rabbit | IgG | Y59 | Abcam | ab32527 | Unconjugated | AB_726208 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32248 | Primary Antibody | CCR7 | Human | Goat | IgG | Polyclonal | Novus Biologicals | NB100-712SS | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P51686 | Primary Antibody | CCR9 | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-33407 | Unconjugated | AB_2550787 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P51686 | Primary Antibody | CCR9 | Human | Mouse | IgG2a | 112509 | Novus Biologicals | FAB179G-100UG | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 103924 | PE | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Rat | IgG2b | 200103 | R&D Systems | MAB1126-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 1039134 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | BioLegend | 312204 | PE | AB_314915 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 103924 | PE | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | BioLegend | 312204 | PE | AB_314915 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 103901 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | BioLegend | 312204 | PE | AB_314915 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P38570 | Primary Antibody | CD103 | Human | Rabbit | IgG | ITGAE/3904R | Novus Biologicals | NBP3-07530-100ug | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P38570 | Primary Antibody | CD103 | Human | Rabbit | IgG | ITGAE/3904R | Novus Biologicals | NBP3-08855AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17813 | Primary Antibody | CD105 (Endoglin) | Human | Mouse | IgG1 | SN6h | BioLegend | 800505 | FITC | AB_2687058 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P19320 | Primary Antibody | CD106 | Human | Mouse | IgG1 | STA | BioLegend | 305806 | PE | AB_314561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P19320 | Primary Antibody | CD106 | Human | Mouse | IgG1 | STA | BioLegend | 305806 | PE | AB_314561 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29533 | Primary Antibody | CD106 | Mouse | Rat | IgG2a | 429 | Thermo Fisher Scientific | 50-1061-82 | eF660 | AB_11219864 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29533 | Primary Antibody | CD106 | Mouse | Rat | IgG2a | 429 | BioLegend | 105710 | AF488 | AB_493427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05532 | Primary Antibody | CD117 | Mouse | Rat | IgG2b | 2B8 | BioLegend | 105827 | BV421 | AB_10898120 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| O97799 | Primary Antibody | CD117 | Canine | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | BS-0672R | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| P10721 | Primary Antibody | CD117 | Human | Mouse | IgG1 | 104D2 | BioLegend | 313203 | PE | AB_314983 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P10721 | Primary Antibody | CD117 | Human | Mouse | IgG1 | 104D2 | BioLegend | 313234 | AF488 | AB_2566221 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9UQ88 | Primary Antibody | CD11a | Human | Mouse | IgG1 | HI111 | AAT Bioquest | 101100C0 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | Abcam | ab187537 (Unconjugated) | AF647 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711PE | PE | AB_323678 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101208 | PE | AB_312791 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101208 | PE | AB_312791 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | Abcam | ab187537 (Unconjugated) | AF647 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | SP331 | Abcam | ab238794 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BD Biosciences | 557397 | PE | AB_396680 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | Abcam | ab187537 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | SP330 | Abcam | ab241408 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BD Biosciences | 101217 | PE | AB_396680 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-8909-5039 | NA | 0000-0002-8909-5039 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD11b | Canine | Mouse | IgG1 | CA16.3E10 | Thermo Fisher Scientific | MA5-16604 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB110-89474AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EPR1344 | Abcam | ab133357 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101254 | AF594 | AB_2563231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 555392 | PE | AB_395793 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561355 | FITC | AB_10611872 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117308 | PE | AB_313777 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117346 | AF594 | AB_2563323 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | IgG1 | 3.9 | BioLegend | 301603 | FITC | AB_314173 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 562561 | BV421 | AB_2737656 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117371 | Spark Red 718 | AB_2924457 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 563026 | BV510 | AB_2737960 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117314 | AF647 | AB_492850 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| P20702 | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | Abcam | ab216655 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | Abcam | ab216655 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_492850 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P20702 | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | Abcam | ab216655 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2780 | Unconjugated | AB_442186 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356517 | BV510 | AB_2562661 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356524 | AF647 | AB_2564250 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356524 | AF647 | AB_2564250 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BioLegend | 142526 | AF647 | AB_2566239 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BD Biosciences | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | AAT Bioquest | 113800C0 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BD Biosciences | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BD Biosciences | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08571 | Primary Antibody | CD14 | Human | Rabbit | IgG | EPR3653 | Abcam | ab226121 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P08571 | Primary Antibody | CD14 | Human | Mouse | IgG1 | 61D3 | Thermo Fisher Scientific | 58-0149-41 | AF532 | AB_11218093 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08571 | Primary Antibody | CD14 | Human | Rabbit | IgG | EPR3653 | Abcam | ab226121 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P33151 | Primary Antibody | CD144 (VE-Cadherin) | Human | Mouse | IgG2a | BV9 | BioLegend | 348513 | AF647 | AB_2563669 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BioLegend | 394702 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BioLegend | 394702 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1% PFA Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BioLegend | 394702 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| A0A0B4J1G0 | Primary Antibody | CD16.2 | Mouse | Armenian Hamster | IgG | 9E9 | BioLegend | 149503 | PE | AB_2565810 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P08101 | Primary Antibody | CD16/32 | Mouse | Goat | IgG | Polyclonal | R&D Systems | AF1460-SP | Unconjugated | NA | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333611 | BV421 | AB_2562462 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | Thermo Fisher Scientific | MA5-11458 | Unconjugated | AB_10982556 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | Thermo Fisher Scientific | MA5-11458 | Unconjugated | AB_10982556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | Thermo Fisher Scientific | 12-1639-41 | PE | AB_1963571 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | Thermo Fisher Scientific | MA5-11458 | Unconjugated | AB_10982556 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333628 | BV510 | AB_2650632 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG2b | 6D3.2F3 | Novus Biologicals | NBP2-36495AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| F7DAU3 | Primary Antibody | CD163 | Rhesus Macaque | Mouse | IgG2b | EDHu-1 | Novus Biologicals | NB110-40686AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG2b | EPR19518 | Abcam | ab213612 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333602 (Unconjugated) | AF532 | AB_2892759 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | EDHu-1 | Novus Biologicals | NB110-40686AF647 | AF647 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333602 (Unconjugated) | AF532 | AB_2892759 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13740 | Primary Antibody | CD166 | Human | Mouse | IgG1 | 3A6 | BioLegend | 343903 | PE | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q13740 | Primary Antibody | CD166 | Human | Rabbit | IgG | EPR2759 | Abcam | ab197543 | AF488 | AB_2889210 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142416 | AF594 | AB_2565620 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | Bio-Rad | MCA884F | FITC | AB_324246 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P49682 | Primary Antibody | CD183 (CXCR3) | Human | Mouse | IgG1 | G025H7 | BioLegend | 353711 | AF647 | AB_10962946 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P49682 | Primary Antibody | CD183 (CXCR3) | Human | Rabbit | IgG | Polyclonal | Abcam | ab154033 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P49682 | Primary Antibody | CD183 (CXCR3) | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-16044 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 558112 | AF488 | AB_397034 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P25918 | Primary Antibody | CD19 | Mouse | Rat | IgG2a | 6D5 | BioLegend | 115552 | AF594 | AB_2563459 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P15391 | Primary Antibody | CD19 | Human | Mouse | IgG1 | SJ25C1 | Thermo Fisher Scientific | 58-0198-41 | AF532 | AB_11219076 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331529 | AF700 | AB_2563656 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331522 | AF488 | AB_10720182 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | AAT Bioquest | 10013150 | AF555 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331506 | PE | AB_1088999 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331525 | BV421 | AB_10933249 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331506 | PE | AB_1088999 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331510 | AF647 | AB_1186032 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11609 | Primary Antibody | CD1d | Mouse | Rat | IgG2b | 1B1 | BD Biosciences | 553846 | PE | AB_2073521 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2b | 2H7 | BioLegend | 302322 | AF700 | AB_493753 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2b | 2H7 | BioLegend | 302354 | AF594 | AB_2565619 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Novus Biologicals | NBP2-80486AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q3C2E2 | Primary Antibody | CD20 | Canine | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| Q3C2E2 | Primary Antibody | CD20 | Canine | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Novus Biologicals | NBP2-80486AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | Yes | 0000-0002-6604-2065 | NA | 0000-0002-6604-2065 |
| P19437 | Primary Antibody | CD20 | Mouse | Rat | IgG2b | SA275A11 | BioLegend | 150432 | AF488 | AB_2890699 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9ES57 | Primary Antibody | CD200 | Mouse | Rat | IgG2a | OX-90 | BD Biosciences | 565547 | BV421 | AB_2739289 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 |
| P22897 | Primary Antibody | CD206 | Human | Mouse | IgG2b | 685645 | R&D Systems | MAB25341 | Unconjugated | AB_10890782 | Stock | Multiplexed 2D Imaging | FFPE | Lung | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141710 | AF488 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141712 | AF647 | AB_10900420 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141709 | AF488 | AB_10933252 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 |
| P22897 | Primary Antibody | CD206 | Human | Mouse | IgG1 | 15-2 | BioLegend | 321130 | PE/Dazzle AF594 | AB_2616867 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q8VBX4 | Primary Antibody | CD207 | Mouse | Rat | IgG2a | eBioL31 | Thermo Fisher Scientific | 12-2075-80 | PE | AB_763453 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354909 | FITC | AB_2561574 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab240987 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD21 | Mouse | Rat | IgG2a | 7.E.9 | BioLegend | 123413 | Pacific Blue | AB_2085159 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354903 | PE | AB_2561406 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab240987 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | CR2/1952 | Novus Biologicals | NBP2-60733AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF488 | NA | Custom | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | 1F8 | Novus Biologicals | NBP1-22527 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338521 | BV421 | AB_2562460 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338505 | FITC | AB_1501125 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20693 | Primary Antibody | CD23 | Mouse | Rat | IgG2a | B3B4 | BioLegend | 101611 | AF647 | AB_493479 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-8909-5039 | NA | 0000-0003-4379-8967 |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01589 | Primary Antibody | CD25 | Human | Mouse | IgG1 | M-A251 | BioLegend | 356128 | AF647 | AB_2563588 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01589 | Primary Antibody | CD25 | Human | Mouse | IgG1 | M-A251 | BioLegend | 356128 | AF647 | AB_2563588 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01590 | Primary Antibody | CD25 | Mouse | Rat | IgG1 | PC61.5 | Thermo Fisher Scientific | 53-0251-82 | AF488 | AB_763472 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01589 | Primary Antibody | CD25 | Human | Mouse | IgG2b | CD25-4E3 | Thermo Fisher Scientific | 17-0257-42 | APC | AB_11218671 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08138 | Primary Antibody | CD271 (NGFR5) | Human | Mouse | IgG1 | NGFR5 | Thermo Fisher Scientific | MA5-13311 | Biotin | AB_10982271 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08138 | Primary Antibody | CD271 (NGFR5) | Human | Mouse | IgG1 | NGRF5 | BioLegend | 818201 | Unconjugated | AB_2564818 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9EP73 | Primary Antibody | CD274 (PD-L1) | Mouse | Rat | IgG2b | MIH1 | BD Biosciences | 753201 | RY586 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P09055 | Primary Antibody | CD29 | Mouse | Armenian Hamster | IgG | HMB1-1 | BioLegend | 102214 | AF647 | AB_492831 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300401 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | AAT Bioquest | 10032060 | iF514 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967, 0009-0006-9784-2694 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | CAL57 | Cell IDx | HI06B-005 | UT016 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100281 | Spark Red 718 | AB_2924440 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-1130-1899 | NA | 0000-0003-1130-1899 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300435 | BV570 | AB_10898117 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Novus Biologicals | NB100-2726IR | DL755 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300448 | BV510 | AB_2563468 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300487 | Spark Blue 574 | AB_2904329 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100205 | PE | AB_312662 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100234 | BV510 | AB_2562555 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | Thermo Fisher Scientific | 58-0032-80 | AF532 | AB_11220474 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300483 | Spark Violet 538 | AB_2890733 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100228 | BV421 | AB_2562553 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100275 | Spark Blue 574 | AB_2904266 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100214 | Pacific Blue | AB_493645 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300415 | AF488 | AB_389310 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300408 | PE | AB_314062 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300402 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Thermo Fisher Scientific | 58-0038-42 | AF532 | AB_11218675 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| G7NCB9 | Primary Antibody | CD3 | Rhesus Macaque | Mouse | IgG1 | F7.2.38 | Agilent | M7254 | Unconjugated | AB_2631163 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300448 | BV510 | AB_2563468 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300416 | AF647 | AB_389332 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100281 | Spark Red 718 | AB_2924440 | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P27597 | Primary Antibody | CD3 | Canine | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100281 | Spark Red 718 | AB_2924440 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Skin | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EPR4426 | Abcam | ab208514 | AF555 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300415 | AF488 | AB_389310 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | Thermo Fisher Scientific | 58-0032-80 | AF532 | AB_11220474 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102516 | AF647 | AB_2161029 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303126 | AF594 | AB_2563303 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303133 | AF700 | AB_2566326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303110 | AF488 | AB_493074 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | Novus Biologicals | NBP2-47785AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | Novus Biologicals | NBP2-47785AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | Novus Biologicals | NBP2-47785AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102515 | AF647 | AB_2161030 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| A0A5F7Z8X0 | Primary Antibody | CD31 | Rhesus Macaque | Rabbit | IgG | Polyclonal | Abcam | ab28364 | Unconjugated | AB_726362 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303112 | AF647 | AB_493076 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303102 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303133 | AF700 | AB_2566326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102514 | AF488 | AB_2161031 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102516 | AF647 | AB_2161029 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0009-0006-9784-2694 | NA | 0009-0006-9784-2694 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Novus Biologicals | FAB7227T | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Novus Biologicals | FAB7227T | AF594 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333406 | PE | AB_2292231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333406 | PE | AB_2292231 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BD Biosciences | 565329 | AF647 | AB_2739186 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333404 | FITC | AB_2085022 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333406 | PE | AB_2292231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28907 | Primary Antibody | CD38 | Human | Mouse | IgG1 | HIT2 | BioLegend | 303503 | FITC | AB_314355 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56528 | Primary Antibody | CD38 | Mouse | Rat | IgG2a | 90 | BioLegend | 102732 | BV421 | AB_2734153 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P28907 | Primary Antibody | CD38 | Human | Mouse | IgG1 | HIT2 | BioLegend | 303524 | AF700 | AB_2072781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P55772 | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | BioLegend | 328205 | FITC | AB_940423 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P55772 | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | BioLegend | 328208 | PE | AB_940429 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P55772 | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | BioLegend | 328205 | FITC | AB_940423 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | Bio-Rad | MCA463SBV670 | StarBright Violet 670 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P22646 | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | Bio-Rad | MCA463SBB700 | StarBright Blue 700 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P22646 | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | Bio-Rad | MCA463SBV710 | StarBright Violet 710 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA1211 | Miltenyi Biotec | 130-123-209 | VioGreen | AB_2811462 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-118-568 | VioBlue | AB_2733943 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-118-794 | PerCP-Vio 700 | AB_2733032 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-118-316 | PE-Vio 615 | AB_2728077 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-123-894 | PE-Vio 770 | AB_2811571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-119-132 | APC-Vio 770 | AB_2733118 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA1211 | Miltenyi Biotec | 130-128-234 | Vio Bright R720 | AB_2905066 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | AAT Bioquest | 10041090 | iF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | AF-379-SP | Unconjugated | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | Novus Biologicals | NBP2-27216AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | Thermo Fisher Scientific | 58-0049-42 | AF532 | AB_2802361 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16003 | Primary Antibody | CD4 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | FAB8165N | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300526 | AF700 | AB_493743 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16003 | Primary Antibody | CD4 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300519 | AF488 | AB_389311 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100443 | BV421 | AB_2562557 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BD Biosciences | 553730 | PE | AB_396634 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | Yes | 0000-0002-6604-2065 | NA | 0000-0002-6604-2065 |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| NA | Primary Antibody | CD4 | Canine | Rat | IgG2a | YKIX302.9 | Thermo Fisher Scientific | MA5-28357 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | Thermo Fisher Scientific | 58-0049-42 | AF532 | AB_2802361 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Spleen | Healthy | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Cell IDx | HI06B-005 | UT019 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 58-0042-80 | AF532 | AB_11219484 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BD Biosciences | 557667 | AF488 | AB_396779 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 58-0042-80 | AF532 | AB_11219484 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BD Biosciences | 557667 | AF488 | AB_396779 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | GK1.5 | BioLegend | 100446 | AF594 | AB_2563182 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 41-0042-82 | eF570 | AB_2573595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 41-0042-82 | eF570 | AB_2573595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100443 | BV421 | AB_2562557 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | AF-379-SP | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | 0000-0002-1461-0999 | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | AAT Bioquest | 104410C0 | iF594 | AB_2935685 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103026 | AF700 | AB_493713 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | No | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | NA | IM7 | AAT Bioquest | 104410C1 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103026 | AF700 | AB_493713 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103007 | PE | AB_312958 | Stock | Multiplexed 2D Imaging | FFPE | Thymus | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103074 | Spark YG 570 | AB_2892265 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103054 | AF594 | AB_2563183 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Infected | Akoya Antibody Diluent | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-9189-7172 | NA | 0000-0001-9189-7172 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Novus Biologicals | NB100-77417IR | DL755 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-9189-7172 | NA | 0000-0001-9189-7172 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | BioLegend | 304018 | AF647 | AB_389336 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016184 | PE/iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Uterus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2634-6838 | NA | 0000-0003-2634-6838 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016184 | PE/iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | Thermo Fisher Scientific | 58-0459-41 | AF532 | AB_11218084 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103144 | AF594 | AB_2563458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0009-0006-9784-2694 | NA | 0009-0006-9784-2694 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103122 | AF488 | AB_493531 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103138 | BV510 | AB_2563061 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103134 | BV421 | AB_2562559 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528PE | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0009-0006-9784-2694 | NA | 0009-0006-9784-2694 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103134 | BV421 | AB_2562559 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103172 | Spark YG 570 | AB_2860598 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103124 | AF647 | AB_493533 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | BioLegend | 304060 | AF594 | AB_2629599 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103144 | AF594 | AB_2563458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528PE | PE | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103124 | AF647 | AB_493533 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | Thermo Fisher Scientific | 58-0459-41 | AF532 | AB_11218084 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11 + PD7/26 | Novus Biologicals | NBP2-34528AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103122 | AF488 | AB_493531 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103183 | Spark Blue 574 | AB_2904274 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | No | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103183 | Spark Blue 574 | AB_2904274 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| NA | Primary Antibody | CD45.1 | Mouse | Mouse | IgG2a | A20 | BioLegend | 110718 | AF488 | AB_492862 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-8909-5039 | NA | 0000-0002-8909-5039 |
| NA | Primary Antibody | CD45.2 | Mouse | Mouse | IgG2a | 104 | BioLegend | 109850 | AF594 | AB_2629589 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| NA | Primary Antibody | CD45.2 | Mouse | Mouse | IgG2a | 104 | BioLegend | 109818 | AF647 | AB_492870 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| NA | Primary Antibody | CD45RA | Human | Mouse | IgG1 | 5H9 | BD Biosciences | 556626 | FITC | AB_396498 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328308 | FITC | AB_2129084 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | CL7207 | Abcam | ab243032 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD49a | Rhesus Macaque | Mouse | IgG1 | CL7207 | Abcam | ab243032 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328303 | PE | AB_1236407 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328303 | PE | AB_1236441 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | CL7207 | Abcam | ab243032 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353125 | AF700 | AB_2810560 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | BioLegend | 304611 | AF488 | AB_493179 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | BioLegend | 304605 | PE | AB_314447 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Ileum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | BioLegend | 304603 | FITC | AB_314445 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3 | Bio-Rad | MCA2693A488T | AF488 | AB_1102209 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3.D5 | Novus Biologicals | NBP2-33132AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3.D5 | Novus Biologicals | NBP2-33132AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| F7DRX3 | Primary Antibody | CD57 | Human | Mouse | IgM | HNK-1 | BioLegend | 359602 | Unconjugated | AB_2562403 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| F7DRX3 | Primary Antibody | CD57 | Human | Mouse | IgM | HNK-1 | BioLegend | 359602 | Unconjugated | AB_2562403 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05106 | Primary Antibody | CD61 | Human | Mouse | IgG1 | Y2/51 | Miltenyi Biotec | 130-098-683 | FITC | AB_2660493 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139304 | PE | AB_10612740 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139322 | AF647 | AB_2566561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 |
| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139322 | AF647 | AB_2566561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P13688 | Primary Antibody | CD66abce | Rhesus Macaque | Mouse | IgG2b | Tet2 | Miltenyi Biotec | 130-116-522 | FITC | AB_2727588 | Stock | Opal-plex | 10% Formalin for 7 Days | Lung | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| P31997 | Primary Antibody | CD66b | Human | Mouse | IgM | G10F5 | Caprico Biotechnologies | 1075136 | iF594 | AB_2892744 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31997 | Primary Antibody | CD66b | Human | Mouse | IgM | G10F5 | BioLegend | 305110 | AF647 | AB_2563171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Uterus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2634-6838 | NA | 0000-0003-2634-6838 |
| A0A8C0Q9Z4 | Primary Antibody | CD68 | Canine | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 AF647 | AF647 | AB_3073741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 AF647 | AF647 | AB_3073741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 AF647 | AF647 | AB_3073741 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 AF647 | AF647 | AB_3073741 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137011 | AF488 | AB_2074847 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | BioLegend | 310918 | AF647 | AB_528871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | BioLegend | 310906 | PE | AB_314841 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3218-4479 | NA | 0000-0002-3218-4479 |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG2a | 15B5G2 | Novus Biologicals | NBP2-25236AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | BioLegend | 310918 | AF647 | AB_528871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q61503 | Primary Antibody | CD73 | Mouse | Rat | IgG1 | TY/11.8 | BioLegend | 127230 | AF700 | AB_2800625 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-2634-6838 | NA | 0000-0003-2634-6838 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100724 | AF647 | AB_389326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | RPA-T8 | Thermo Fisher Scientific | 58-0088-24 | AF532 | AB_11220473 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BD Biosciences | 553032 | PE | AB_394571 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Skin | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Heart | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 |
| NA | Primary Antibody | CD8 | Canine | Rat | IgG1 | YCATE55.9 | Thermo Fisher Scientific | MA5-28430 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | RPA-T8 | BD Biosciences | 557945 | AF700 | AB_396953 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100752 | BV510 | AB_2563057 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | AAT Bioquest | 100810C0 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100723 | AF488 | AB_389304 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BD Biosciences | 553032 | PE | AB_394571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | Thermo Fisher Scientific | 14-0085-82 | Unconjugated | AB_11150240 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100758 | AF594 | AB_2563237 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01732 | Primary Antibody | CD8 | Human | Rabbit | IgG | EPR10640 | Cell IDx | HI06B-005 | UT015 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967, 0009-0006-9784-2694 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372910 | Spark YG 570 | AB_2904405 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100724 | AF647 | AB_389326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P33681 | Primary Antibody | CD80 | Human | Mouse | IgG1 | 2D10 | BioLegend | 305215 | AF647 | AB_528874 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P33681 | Primary Antibody | CD80 | Human | Mouse | IgG2b | 62N3G8 | Novus Biologicals | NBP2-25255AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P33681 | Primary Antibody | CD80 | Human | Goat | IgG | Polyclonal | R&D Systems | AF140 | Unconjugated | AB_354778 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P35762 | Primary Antibody | CD81 | Mouse | Armenian Hamster | IgG1 | Eat-2 | BioLegend | 104905 | PE | AB_2076267 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-8909-5039 | NA | 0000-0002-8909-5039 |
| Q01151 | Primary Antibody | CD83 | Human | Mouse | IgG1 | HB15e | BioLegend | 305314 | AF488 | AB_528877 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q01151 | Primary Antibody | CD83 | Human | Mouse | IgG1 | HB15e | BD Biosciences | 550634 | PE | AB_393794 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD86 | Mouse | Rat | IgG2a | GL-1 | BioLegend | 105020 | AF647 | AB_493465 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | No | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| P42081 | Primary Antibody | CD86 | Human | Goat | IgG | Polyclonal | R&D Systems | AF-141-NA | Unconjugated | AB_354385 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P42081 | Primary Antibody | CD86 | Human | Mouse | IgG2b | IT2.2 | BioLegend | 305415 | AF647 | AB_528882 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P42082 | Primary Antibody | CD86 (B7-2) | Mouse | Rat | IgG2a | GL1 | R&D Systems | FAB741S-100UG | AF750 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P01732 | Primary Antibody | CD8a | Mouse | Mouse | IgG1 | HIT8a | BioLegend | 300908 | PE | AB_314112 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P01731 | Primary Antibody | CD8a | Mouse | Rat | IgG2a | 53-6.7 | R&D Systems | FAB116S-100UG | AF750 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P01831 | Primary Antibody | CD90.1 | Mouse | Mouse | IgG1 | OX-7 | BioLegend | 202508 | AF647 | AB_492884 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305506 | AF488 | AB_314536 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305504 | FITC | AB_314534 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305506 | PE | AB_314536 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305506 | PE | AB_314536 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305512 | AF647 | AB_2249691 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NXV6 | Primary Antibody | CDKN2a | Human | Mouse | IgG2a | ARF 4C6/4 | Santa Cruz Biotechnology | sc-53392 | Unconjugated | AB_630056 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NXV6 | Primary Antibody | CDKN2a | Human | Rat | IgG2b | 5-C3-1 | Novus Biologicals | NB200-174AF647 | AF647 | AB_10002046 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13569 | Primary Antibody | CFTR | Human | Mouse | IgG1 | M3A7 | Thermo Fisher Scientific | MA5-11768 | Unconjugated | AB_10983660 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P13569 | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | Thermo Fisher Scientific | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P13569 | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | Thermo Fisher Scientific | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| NA | Secondary Antibody | Chicken IgY (H&L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-32932 | AF555 | AB_2762844 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| NA | Secondary Antibody | Chicken IgY (H&L) | Zebrafish | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11039 | AF488 | AB_2534096 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Secondary Antibody | Chicken IgY (H&L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA1-72000 | FITC | AB_923386 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10645 | Primary Antibody | Chromogranin A | Human | Mouse | IgG1 | LK2H10 | Novus Biologicals | NBP2-34672AF532 | AF532 | AB_2892553 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P58304 | Primary Antibody | Chx10 | Mouse | Mouse | IgG2a | E-12 | Santa Cruz Biotechnology | sc-365519 AF546 | AF546 | AB_10842442 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P70194 | Primary Antibody | CLEC4F | Mouse | Goat | IgG | Polyclonal | R&D Systems | AF2784 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| Q6UXN8 | Primary Antibody | CLEC9a | Human | Mouse | IgG2a | 8F9 | BioLegend | 353802 (Unconjugated) | AF488 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BWP8 | Primary Antibody | COLEC11 | Human | Rabbit | IgG | Polyclonal | Proteintech | 15269-1-AP | Unconjugated | AB_10732940 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P02462 | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | Abcam | ab6586 | Unconjugated | AB_305584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02462 | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | Abcam | ab6586 | Unconjugated | AB_305584 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02463 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02463 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02463 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02463 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 |
| P02462 | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | Abcam | ab6586 | Unconjugated | AB_305584 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q6PBM9 | Primary Antibody | Cone Arrestin | Zebrafish | Rabbit | IgG | Polyclonal | MilliporeSigma | AB15282 | Unconjugated | AB_1163387 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q9EQP6 | Primary Antibody | Cone Arrestin | Mouse | Rabbit | IgG | Polyclonal | MilliporeSigma | AB15282 | Unconjugated | AB_1163387 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| Q6FHL8 | Primary Antibody | CTGF | Human | Rabbit | IgG | OT11F4 | FabGennix | CTGF-FITC | FITC | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16410 | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | Abcam | ab270127 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16410 | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | Abcam | ab270127 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0009-0006-9784-2694 | NA | 0009-0006-9784-2694 |
| P16410 | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | Abcam | ab270127 | AF647 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P63158 | Primary Antibody | CXCL12 | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab25117 | NA | AB_2088164 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| Q6ICW0 | Primary Antibody | CXCL12 | Human | Mouse | IgG1 | 79018 | R&D Systems | MAB350-500 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q6ICW0 | Primary Antibody | CXCL12 | Human | Mouse | IgG1 | 79018 | R&D Systems | MAB350 | Unconjugated | AB_2088149 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 (Unconjugated) | AF532 | AB_2892755 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 | Unconjugated | AB_2892755 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 (Unconjugated) | AF532 | AB_2892755 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 | Unconjugated | AB_2892755 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P49682 | Primary Antibody | CXCR3 | Human | Mouse | IgG1 | 1C6/CXCR3 | BD Biosciences | 561320 | AF700 | AB_10611719 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | No | 0000-0002-6604-2065 | NA | 0000-0002-6604-2065 |
| P49682 | Primary Antibody | CXCR3 | Human | Mouse | IgG1 | 49801 | R&D Systems | FAB160N | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | No | 0000-0002-6604-2065 | NA | 0000-0002-6604-2065 |
| P70658 | Primary Antibody | CXCR4 | Mouse | Rabbit | IgG | Polyclonal | Novus Biologicals | NB-100 74396 | NA | AB_1048484 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| Q9EQ16 | Primary Antibody | CXCR6 | Mouse | Rat | IgG2b | FAB2145R | Novus Biologicals | 221002 | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P56485 | Primary Antibody | CXCR7 | Mouse | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-24779G | DL488 | NA | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| NA | Primary Antibody | Cytokeratin | Mouse | Mouse | IgG1 | C-11 | BioLegend | 628604 | AF647 | AB_2563652 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin | Mouse | Mouse | IgG1 | C-11 | BioLegend | 628608 | AF488 | AB_2563652 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 50-9003-82 | eF660 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | BioLegend | 914204 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 41-9003-80 | eF570 | AB_11217482 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Novus Biologicals | NBP2-33200AF750 | AF750 | AB_2868569 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| NA | Primary Antibody | Cytokeratin (Pan) | Canine | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Novus Biologicals | NBP2-33200AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 50-9003-82 | eF660 | AB_2574301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 42-9003-82 | eF615 | AB_10804663 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13645 | Primary Antibody | Cytokeratin 10 | Human | Mouse | IgG1 | DE-K10 | Novus Biologicals | NBP2-54402AF488 | AF488 | AB_2892746 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P02533 | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 | Unconjugated | AB_2616962 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P02533 | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 | Unconjugated | AB_2616962 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02533 | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 (Unconjugated) | AF647 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05784 | Primary Antibody | Cytokeratin 18 | Mouse | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-14263 | Unconjugated | AB_2133178 | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| P08729 | Primary Antibody | Cytokeratin 7 | Human | Rat | IgG2a | W16155A | BioLegend | 601606 | AF488 | AB_2728458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3218-4479 | NA | 0000-0002-3218-4479 |
| NA | Nuclear Dye | DAPI | Zebrafish | NA | NA | NA | Thermo Fisher Scientific | D1306 | NA | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | 9E9A8 | BioLegend | 330112 | AF647 | AB_1186092 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | 9E9A8 | BioLegend | 330112 | AF647 | AB_1186092 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | LSBio | LS-B3782 | Unconjugated | AB_10689801 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | 102E11.06 | Eurobio Scientific (Dendritics) | DDX0202A647-100 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | 102E11.06 | Eurobio Scientific (Dendritics) | DDX0202A647-100 | AF647 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | C209/1781 | Novus Biologicals | NBP2-54351AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | DC28 | R&D Systems | MAB16211-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | LSBio | LS-B3782 | Unconjugated | AB_10689801 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9JLM8 | Primary Antibody | DCAMKL1 | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab37994 | Unconjugated | AB_873538 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| O15075 | Primary Antibody | DCAMKL1 | Human | Rabbit | IgG | Polyclonal | Abcam | ab31704 | Unconjugated | AB_873537 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| O15075 | Primary Antibody | DCAMKL1 | Human | Rabbit | IgG | EPR6085 | Abcam | ab187184 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q16832 | Primary Antibody | DDR2 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2538 | Unconjugated | AB_416613 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q60767 | Primary Antibody | DEC205 | Mouse | Rat | IgG2a | NLDC-145 | BioLegend | 138204 | AF647 | AB_2137655 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q60767 | Primary Antibody | DEC205 | Mouse | Rat | IgG2a | NLDC-145 | BioLegend | 138204 | AF647 | AB_2137655 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17661 | Primary Antibody | Desmin | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab15200 | Unconjugated | AB_301744 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P09803 | Primary Antibody | E-cadherin | Mouse | Rat | IgG1 | DECMA-1 | BioLegend | 147308 | AF647 | AB_2563955 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P12830 | Primary Antibody | E-cadherin | Human | Rat | IgG1 | DECMA-1 | BioLegend | 147307 | AF647 | AB_2563954 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Nasal Polyp | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0004-8162-409X | NA | 0009-0004-8162-409X |
| P32249 | Primary Antibody | EBI2 | Human | Rabbit | IgG | Polyclonal | RayBiotech | 119-10961 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32249 | Primary Antibody | EBI2 | Human | Mouse | IgG2a | SA313E4 | BioLegend | 368903 | AF647 | AB_2566384 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32249 | Primary Antibody | EBI2 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NLS46 | Unconjugated | AB_10001402 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | EBOV NP | Mouse | Rabbit | NA | Polyclonal | IBT BioServices | 0301-012 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| NA | Primary Antibody | EBOV NP | Mouse | Rabbit | NA | Polyclonal | IBT BioServices | 0301-012 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324228 | AF594 | AB_2563209 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JW5 | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | BioLegend | 118212 | AF647 | AB_1134101 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324210 | AF488 | AB_756084 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324235 | BV510 | AB_2632934 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324228 | AF594 | AB_2563209 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324212 | AF647 | AB_756086 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JW5 | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | BD Biosciences | 747748 | BV510 | AB_2872217 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324243 | AF700 | AB_2750488 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q99JW5 | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | BioLegend | 118222 | AF594 | AB_2563322 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324202 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Rabbit | IgG | Polyclonal | Abcam | ab71916 | Unconjugated | AB_1603782 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG1 | 1B7 | Thermo Fisher Scientific | 50-9326-42 | eF660 | AB_10598658 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | ERTR7 | Mouse | Rat | IgG2a | ER-TR7 | Novus Biologicals | NB100-64932IR | DL755 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 |
| Q14246 | Primary Antibody | F4/80 | Mouse | Rat | IgG2b | F4/80 | Abcam | ab204467 | AF647 | AB_2810932 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123130 | AF700 | AB_2293450 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q14246 | Primary Antibody | F4/80 | Mouse | Rat | IgG2b | F4/80 | Abcam | ab204467 | AF647 | AB_2810932 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q3S4B0 | Primary Antibody | F4/80 | Mouse | Rabbit | IgG | D2S9R | Cell Signaling Technology | 70076 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | Thermo Fisher Scientific | 12-4801-82 | PE | AB_465923 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123132 | BV421 | AB_11203717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q3S4B0 | Primary Antibody | F4/80 | Mouse | Rabbit | IgG | D2S9R | Cell Signaling Technology | 70076 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123122 | AF647 | AB_893480 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123122 | AF647 | AB_893480 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123132 | BV421 | AB_11203717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Blocking Reagent | Fc Block | Human | Human | IgG1 | Fc1 | BD Biosciences | 564220 | Unconjugated | AB_2869554 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Blocking Reagent | Fc Block | Mouse | Rat | IgG2b | 2.4G2 | BD Biosciences | 553141 | Unconjugated | AB_394656 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8TBJ5 | Primary Antibody | FEZF2 | Human | Rabbit | IgG | Polyclonal | Abcam | ab214186 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P02671 | Primary Antibody | Fibrinogen | Human | Sheep | IgG | Polyclonal | Abcam | ab118533 | Unconjugated | AB_10900171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02671 | Primary Antibody | Fibrinogen | Human | Sheep | IgG | Polyclonal | Abcam | ab118533 | Unconjugated | AB_10900171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q12884 | Primary Antibody | Fibroblast activation protein alpha | Human | Rabbit | IgG | Polyclonal | MilliporeSigma | ABT11 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02751 | Primary Antibody | Fibronectin | Human | Mouse | IgG1 | 2F4 | Novus Biologicals | NBP2-22113AF532 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Fluorescein/Oregon Green | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | A-11090 | AF488 | AB_221562 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q12951 | Primary Antibody | FOXI1 | Human | Mouse | IgG1 | OTI1D4 | Novus Biologicals | NBP2-70747AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q9H334 | Primary Antibody | FOXP1 | Human | Mouse | IgG2a | JC12 | Novus Biologicals | NB100-65125 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 58-5773-82 | AF532 | AB_11218870 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Rabbit | IgG | 1054C | Cell IDx | HI06B-005 | UT014 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 53-5773-82 | AF488 | AB_763537 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 50-4777-42 | eF660 | AB_2574219 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 53-4777-41 | AF488 | AB_10852836 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 13-4777-82 | Biotin | AB_466655 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 12-5773-82 | PE | AB_465936 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 14-4777-82 | Unconjugated | AB_467556 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 14-4777-82 | Unconjugated | AB_467556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS2 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 17-4777-42 | APC | AB_10804651 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| Q9BZS2 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 17-4777-42 | APC | AB_10804651 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 41-5773-82 | eF570 | AB_11219073 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Francisella Tularensis, LPS | Mouse | Mouse | IgG2a | 1.B.288 | US Biological Life Sciences | F6070-02X | Unconjugated | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | NA | Yes | 0000-0001-9189-7172 | NA | 0000-0001-9189-7172 |
| Q14392 | Primary Antibody | GARP | Human | Mouse | IgG2b | 7B11 | BioLegend | 352502 | Unconjugated | AB_10897447 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q14392 | Primary Antibody | GARP | Human | Mouse | IgG2b | Plato-1 | Enzo | ALX-804-867-C100 | Unconjugated | AB_2051806 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q14392 | Primary Antibody | GARP | Human | Mouse | IgG2b | 7B11 | BioLegend | 352503 | PE | AB_10896792 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P23771 | Primary Antibody | GATA-3 | Human | Mouse | IgG1 | L50-823 | BD Biosciences | 560077 | AF488 | AB_1645303 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P03995 | Primary Antibody | GFAP | Mouse | Mouse | IgG1 | GA5 | Thermo Fisher Scientific | 41-9892-82 | eF570 | AB_2573656 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| P14136 | Primary Antibody | GFAP | Mouse | Mouse | IgG2b | SMI 25 | BioLegend | 837504 | AF647 | AB_2632641 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| Q58EE9 | Primary Antibody | GFAP | Zebrafish | Mouse | IgG2b | SMI 25 | BioLegend | 837508 | AF488 | AB_2728555 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P42212 | Primary Antibody | GFP | Jellyfish GFP transgene | Chicken | IgY | Monoclonal | Thermo Fisher Scientific | A10262 | Unconjugated | AB_2534023 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q76IQ4 | Primary Antibody | Ghrelin | Human | Mouse | IgG2b | 891240 | R&D Systems | MAB8149 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | GL-7 | Mouse | Rat | IgM | GL7 | BD Biosciences | 561530 | PE | AB_10715834 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | GL7 | Mouse | Rat | IgM | GL7 | BD Biosciences | 561530 | PE | AB_10715834 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Rabbit | IgG | Polyclonal | Abcam | ab49873 | Unconjugated | AB_880241 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P51121 | Primary Antibody | Glutamine synthetase | African Clawed Frog (Xenopus laevis) | Mouse | IgG1 | 1B6G4 | Proteintech | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Rabbit | IgG | EPR13022(B) | Abcam | ab302584 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2a | OTI1F4 | Novus Biologicals | NBP2-70834AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| A0A8C6NQP0 | Primary Antibody | Glutamine synthetase | Turquoise Killifish | Mouse | IgG1 | 1B6G4 | Proteintech | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q7ZVF2 | Primary Antibody | Glutamine synthetase | Zebrafish | Mouse | IgG1 | 1B6G4 | Proteintech | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2a | OTI1F4 | Novus Biologicals | NBP2-70834AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15104 | Primary Antibody | Glutamine synthetase | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab49873 | Unconjugated | AB_880241 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P15104 | Primary Antibody | Glutamine synthetase | Mouse | Mouse | IgG1 | 1B6G4 | Proteintech | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2bc | GLUL/6600 | Novus Biologicals | NBP3-14023AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02724 | Primary Antibody | Glycophorin A | Human | Rabbit | IgG | EPR8200 | Abcam | ab218372 (Unconjugated) | AF555 | AB_2892758 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02724 | Primary Antibody | Glycophorin A | Human | Rabbit | IgG | EPR8200 | Abcam | ab218372 (Unconjugated) | AF555 | AB_2892758 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q90WX5 | Primary Antibody | GNAT2 | Zebrafish | Rabbit | IgG | Polyclonal | MBL International Corporation | PM075 | Unconjugated | AB_2864374 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Primary Antibody | Go-alpha | Zebrafish | Mouse | IgG1 | 2A | MilliporeSigma | MAB3073 | Unconjugated | AB_94671 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11056 | AF546 | AB_2534103 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21084 | AF680 | AB_2535741 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21084 | AF680 | AB_2535741 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A32816 | AF555 (Plus) | AB_2762839 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11058 | AF594 | AB_2534105 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11055 | AF488 | AB_2534102 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Abcam | ab175745 | AF750 | AB_2924800 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21447 | AF647 | AB_2535864 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Canine | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21447 | AF647 | AB_2535864 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10091 | DL755 | AB_2556671 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-32930 | AF800 (Plus) | AB_2762842 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21084 | AF680 | AB_2535741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11055 | AF488 | AB_2534102 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10091 | DL755 | AB_2556671 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11055 | AF488 | AB_2534102 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62011 | Primary Antibody | gp38 | Mouse | Syrian Hamster | IgG | 8.1.1 | BioLegend | 127401 | Unconjugated | AB_1089186 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| F7CLT4 | Primary Antibody | Granzyme B | Rhesus Macaque | Mouse | IgG2a | GrB-7 | Agilent | M7235 | Unconjugated | AB_2114697 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P10144 | Primary Antibody | Granzyme B | Human | Rabbit | IgG | Polyclonal | Abcam | ab4059 | Unconjugated | AB_304251 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10144 | Primary Antibody | Granzyme B | Human | Rabbit | IgG | EP230 | Cell Marque | 262R-17 | Unconjugated | AB_2889344 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Granzyme B | Canine | Mouse | IgG1 | GB12 | Thermo Fisher Scientific | MHGB04 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| NA | Secondary Antibody | Hamster IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21451 | AF647 | AB_2535868 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q64525 | Primary Antibody | Histone H2B | Mouse | Rat | IgG2a | W17025A | BioLegend | 606301 | Unconjugated | AB_2728465 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P02302 | Primary Antibody | Histone H3 | Mouse | Mouse | IgG3 | 1B1-B2 | BioLegend | 819406 | AF594 | AB_2715803 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P68431 | Primary Antibody | Histone H3 (citrulline R2 + R8 + R17) | Human | Rabbit | IgG | Polyclonal | Abcam | ab5103 | Unconjugated | AB_304752 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307602 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307625 | AF700 | AB_493770 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Abcam | ab20181 | AF647 | AB_445401 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Caprico Biotechnologies | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Caprico Biotechnologies | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670PE | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307622 | AF647 | AB_493177 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307619 | AF488 | AB_493176 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Caprico Biotechnologies | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670 | PE | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Abcam | ab176408 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307622 | AF647 | AB_493177 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Novus Biologicals | NB600-989AF532-0.1ml | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | HLA-DR | Canine | Mouse | IgG1 | TAL 1B5 | Abcam | ab176408 (Unconjugated) | iF594 | NA | Custom | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Novus Biologicals | NB100-77855AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Abcam | ab176408 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01911 | Primary Antibody | HLA-DRB | Human | Mouse | IgG2b | SPM288 | Novus Biologicals | NBP2-45312-0.02mg | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P63158 | Primary Antibody | HMGB-1 | Mouse | Mouse | IgG2 | 3E8 | BioLegend | 651406 | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | Hoechst 33342 | Rhesus Macaque | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | HuC/D | Turquoise Killifish | Mouse | IgG2b | 16A11 | Thermo Fisher Scientific | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Primary Antibody | HuC/D | African Clawed Frog (Xenopus laevis) | Mouse | IgG2b | 16A11 | Thermo Fisher Scientific | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Primary Antibody | HuC/D | Zebrafish | Mouse | IgG2b | 16A11 | Thermo Fisher Scientific | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q14576 | Primary Antibody | HuC/HuD neuronal protein | Human | Mouse | IgG2b | 16A11 | Thermo Fisher Scientific | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| Q9EQX4 | Primary Antibody | Iba-1 | Mouse | Rabbit | IgG | Polyclonal | FUJIFILM Wako Chemicals U.S.A. Corporation | 019-19741 | NA | AB_839504 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| P55008 | Primary Antibody | Iba1 | Human | Goat | IgG | Polyclonal | Abcam | ab5076 | Unconjugated | AB_2224402 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| Q9Y6W8 | Primary Antibody | ICOS | Human | Rabbit | IgG | SP98 | Abcam | ab105227 | Unconjugated | AB_10710236 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9Y6W8 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313515 | AF647 | AB_2122583 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9Y6W8 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313514 | AF488 | AB_2122584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9Y6W8 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313527 | AF700 | AB_2566125 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9Y6W8 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313514 | AF488 | AB_2122584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01878 | Primary Antibody | IgA | Mouse | Goat | IgG | Polyclonal | SouthernBiotech | 1040-32 | AF555 | AB_2794378 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01878 | Primary Antibody | IgA | Mouse | Goat | IgG | Polyclonal | SouthernBiotech | 1040-32 | AF555 | AB_2794378 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01876 | Primary Antibody | IgA1 | Human | Mouse | IgG1 | B3506B4 | SouthernBiotech | 9130-31 | AF647 | AB_2796658 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01876 | Primary Antibody | IgA1 | Human | Mouse | IgG1 | B3506B4 | SouthernBiotech | 9130-31 | AF647 | AB_2796658 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01877 | Primary Antibody | IgA2 | Human | Mouse | IgG1 | A9604D2 | SouthernBiotech | 9140-30 | AF488 | AB_2796665 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01877 | Primary Antibody | IgA2 | Human | Mouse | IgG1 | A9604D2 | SouthernBiotech | 9140-30 | AF488 | AB_2796665 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405718 | AF488 | AB_10730619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405718 | AF488 | AB_10730619 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01880 | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | BioLegend | 348216 | AF488 | AB_11150595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405723 | BV510 | AB_2562742 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01880 | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | BioLegend | 348216 | AF488 | AB_11150595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01880 | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | BioLegend | 348229 | AF700 | AB_2563334 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405740 | AF594 | AB_2565572 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06336 | Primary Antibody | IgE | Mouse | Rat | IgG1 | RME-1 | BioLegend | 406908 | PE | AB_493290 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P01868 | Primary Antibody | IgG1 | Mouse | Rat | IgG | RMG1-1 | BioLegend | 406616 | BV421 | AB_2562234 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P07476 | Primary Antibody | Involucrin | Human | Mouse | IgG1 | SY5 | Santa Cruz Biotechnology | sc-21748 AF488 | AF488 | AB_2128119 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P07476 | Primary Antibody | Involucrin | Human | Rabbit | IgG | Polyclonal | Abcam | ab53112 | Unconjugated | AB_880813 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| Q15306 | Primary Antibody | IRF4 | Human | Mouse | IgG1 | MUM1p | Novus Biologicals | NB200-356 | Unconjugated | AB_10003502 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15306 | Primary Antibody | IRF4 | Human | Rat | IgG1 | IRF4.3E4 | BioLegend | 646404 | PE | AB_2563005 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15306 | Primary Antibody | IRF4 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP1-82814 | Unconjugated | AB_11038596 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15306 | Primary Antibody | IRF4 | Human | Mouse | IgG1 | 4G10 | Novus Biologicals | NBP1-47396 | Unconjugated | AB_10010349 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15306 | Primary Antibody | IRF4 | Human | Rabbit | IgG | EP5699 | Abcam | ab252024 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q64287 | Primary Antibody | IRF4 | Mouse | Rat | IgG1 | 3.E.4 | Thermo Fisher Scientific | 11-9858-82 | FITC | AB_2572538 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15306 | Primary Antibody | IRF4 | Human | Sheep | IgG | Polyclonal | R&D Systems | AF5525 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15306 | Primary Antibody | IRF4 | Human | Mouse | IgG1 | MUM1p | Novus Biologicals | NB200-356 | Unconjugated | AB_10003502 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15306 | Primary Antibody | IRF4 | Human | Rat | IgG1 | IRF4.3E4 | BioLegend | 646404 | PE | AB_2563005 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q02556 | Primary Antibody | IRF8 | Human | Rabbit | IgG | E6J8Q | Cell Signaling Technology | 80174S | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| NA | Lectin | Isolectin GS-IB4 | Human | NA | NA | NA | Thermo Fisher Scientific | I21412 | AF568 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | Thermo Fisher Scientific | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | Thermo Fisher Scientific | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | Thermo Fisher Scientific | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | Thermo Fisher Scientific | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | Thermo Fisher Scientific | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02533 | Primary Antibody | Keratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 (Unconjugated) | AF647 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13647 | Primary Antibody | Keratin 5 | Human | Rabbit | IgG | Polyclonal | BioLegend | 905504 (Unconjugated) | AF532 | AB_3095835 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Rat | IgG2a | SolA15 | Thermo Fisher Scientific | 48-5698-80 | eF450 | AB_11151155 | Stock | Ce3D-IBEX | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 1 hour | Yes | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 558616 | AF488 | AB_647087 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB500-170b | Biotin | AB_1660247 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB500-170b | Biotin | AB_1660247 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P79192 | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | BD Biosciences | 563462 | BV510 | AB_2738221 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P79192 | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | BD Biosciences | 562899 | BV421 | AB_2686897 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P79192 | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | BD Biosciences | 558616 | AF488 | AB_647087 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB500-170b | Biotin | AB_1660247 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB500-170 | Unconjugated | AB_10001977 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Rat | IgG2a | SolA15 | Thermo Fisher Scientific | 606-5698-82 | AF660 | AB_2896286 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| E9PVX7 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| E9PVX8 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P79192 | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | BD Biosciences | 558615 | AF647 | AB_647130 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| E9PVX9 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 |
| O88713 | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | BD Biosciences | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| O88713 | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | BD Biosciences | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| O88713 | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | BD Biosciences | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18627 | Primary Antibody | LAG-3 | Human | Mouse | IgG1 | 17B4 | Novus Biologicals | NBP1-97657IR | DL755 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18627 | Primary Antibody | LAG-3 | Human | Rabbit | IgG | Polyclonal | LSBio | LS-CS165666 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18627 | Primary Antibody | LAG-3 | Human | Mouse | IgG1 | 17B4 | Novus Biologicals | NBP1-97657 | AF488 | AB_11162489 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02545 | Primary Antibody | Lamin A | Human | Mouse | IgG3 | 133A2 | Thermo Fisher Scientific | MUB1101L1 | FITC | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Colon | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11047 | Primary Antibody | Laminin | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB300-144AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02468, P19137 | Primary Antibody | Laminin 1 + 2 | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab7463 | Unconjugated | AB_305933 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| Q9UJ71 | Primary Antibody | Langerin | Human | Rat | IgG2a | 929F3.01 | Novus Biologicals | DDX0362P-100 | Unconjugated | AB_2892751 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P01137 | Primary Antibody | LAP (TGFB) | Human | Mouse | IgG1 | 27232 | R&D Systems | FAB2463G | AF488 | AB_10718684 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P59901 | Primary Antibody | LILRA4 (CD85g) | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-15012 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| D0PNI2 | Primary Antibody | LOX | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB100-2530AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Tumor | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q12912 | Primary Antibody | LRMP | Human | Rabbit | IgG | EPR17852 | Abcam | ab202418 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 (Unconjugated) | AF680 | AB_2892760 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 (Unconjugated) | iF594 | AB_2892764 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| A0A8C0SV20 | Primary Antibody | Lumican | Canine | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| A0A8C0SV20 | Primary Antibody | Lumican | Canine | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51884 | Primary Antibody | Lumican | Human | Rabbit | IgG | EPR8898(2) | Abcam | ab252012 | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8R2S8 | Primary Antibody | Ly-6G | Mouse | Rat | IgG2a | 1A8 | BioLegend | 127626 | AF488 | AB_2561340 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q6P698 | Primary Antibody | Lymphocyte cytosolic protein 1 (L-plastin/LCP1) | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 13025-1-AP | Unconjugated | AB_2136871 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q6P698 | Primary Antibody | Lymphocyte cytosolic protein 1 (L-plastin/LCP1) | Zebrafish | Rabbit | IgG | Polyclonal | GeneTex | GTX124420 | Unconjugated | AB_11167454 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P61626 | Primary Antibody | Lysozyme | Human | Rabbit | IgG | Polyclonal | Abcam | ab2408 | Unconjugated | AB_303050 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8BHC0 | Primary Antibody | LYVE-1 | Mouse | Rabbit | IgG | E3L3V | Cell Signaling Technology | 67538 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| Q8BHC0 | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | Thermo Fisher Scientific | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8BHC0 | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | Thermo Fisher Scientific | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8BHC0 | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | Thermo Fisher Scientific | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 (Unconjugated) | AF532 | AB_2892756 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| A0A8P0NA08 | Primary Antibody | LYVE-1 | Canine | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| F7AVS7 | Primary Antibody | LYVE-1 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 (Unconjugated) | AF532 | AB_2892756 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9UEW3 | Primary Antibody | MARCO | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-64134 | Unconjugated | AB_2643767 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | mCherrry | Mouse | Chicken | IgY | Polyclonal | Novus Biologicals | NBP2-25158 | NA | AB_2636881 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 |
| A0A8C0S4Q3 | Primary Antibody | Melan-A | Canine | Mouse | IgG1 | rMLANA | Thermo Fisher Scientific | 2315-MSM12-P1 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| Q13421 | Primary Antibody | Mesothelin | Human | Rabbit | IgG | YP158 | Novus Biologicals | NBP2-75255 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Peritoneum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13421 | Primary Antibody | Mesothelin | Human | Mouse | IgG2a | MB-G10 | Novus Biologicals | NB110-85538 | Unconjugated | AB_1199375 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Peritoneum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107618 | AF647 | AB_493525 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107618 | AF647 | AB_493525 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107620 | Pacific Blue | AB_493527 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107631 | BV421 | AB_10900075 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | Thermo Fisher Scientific | 48-5321-82 | eF450 | AB_1272204 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11030 | AF546 | AB_2737024 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21235 | AF647 | AB_2535804 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10175 | DL755 | AB_2556755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21052 | AF633 | AB_2535719 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31570 | AF555 | AB_2536180 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16548 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11030 | AF546 | AB_2534089 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11031 | AF568 | AB_144696 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11002 | AF532 | AB_2534070 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-10038 | AF680 | AB_2534014 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | PA1-28626 | Texas Red | AB_10983944 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Rhesus Macaque | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31570 | AF555 | AB_2536180 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21203 | AF594 | AB_141633 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21202 | AF488 | AB_141607 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Canine | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21203 | AF594 | AB_141633 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21036 | AF700 | AB_2535707 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21236 | AF647 | AB_2535805 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG1 | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21121 | AF488 | AB_2535764 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG1 | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21121 | AF488 | AB_2535764 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Zenon Labeling Kit | Mouse IgG1 | Human | NA | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25011 | AF700 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG1 | Human | Alpaca | IgG1 | CTK0103/CTK0104 | Thermo Fisher Scientific | SA5-10331 | AF568 | AB_2868378 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG2a | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21137 | AF555 | AB_2535776 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG2a | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21137 | AF555 | AB_2535776 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgG2b | Human | Alpaca | IgG2b | CTK0105/CTK0106 | Thermo Fisher Scientific | SA5-10339 | AF647 | AB_2868386 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-545-020 | AF488 | AB_2340844 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-095-140 | FITC | AB_2340791 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-605-020 | AF647 | AB_2340860 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-545-020 | AF488 | AB_2340844 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-605-020 | AF647 | AB_2340860 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-545-020 | AF488 | AB_2340844 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Mycobacterium tuberculosis | Human | Rabbit | IgG | Polyclonal | Abcam | ab905 | Unconjugated | AB_306965 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| NA | Primary Antibody | Mycobacterium tuberculosis Ag85B | Human | Rabbit | IgG | Polyclonal | Abcam | ab43019 | Unconjugated | AB_776575 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | Thermo Fisher Scientific | S11224 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | Thermo Fisher Scientific | 48-4317-82 | eF450 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | BioLegend | 405203 | PE | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | Jackson ImmunoResearch | 016-620-084 | AF680 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | Thermo Fisher Scientific | S21384 | AF750 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | Thermo Fisher Scientific | S21384 | AF750 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | BioLegend | 405235 | AF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | AAT Bioquest | 16956 | iF514 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | FlexAble CoraLite Plus 550 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | Proteintech | KFA002 | CoraLite Plus AF550 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | FlexAble CoraLite Plus 750 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | Proteintech | KFA004 | CoraLite Plus AF750 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Avidin/Biotin Blocking Kit | NA | Human | NA | NA | NA | Abcam | ab64212 | NA | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Avidin/Biotin Blocking Kit | NA | Human | NA | NA | NA | Abcam | ab64212 | NA | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | FlexAble CoraLite Plus 647 Antibody Labeling Kit for Mouse IgG1 | NA | Zebrafish | NA | NA | NA | Proteintech | KFA023 | CoraLite Plus AF647 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | FlexAble CoraLite Plus 555 Antibody Labeling Kit for Mouse IgG1 | NA | Zebrafish | NA | NA | NA | Proteintech | KFA022 | CoraLite Plus AF555 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | FlexAble CoraLite Plus 488 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | Proteintech | KFA001 | CoraLite Plus AF488 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q13562 | Primary Antibody | NeuroD1 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-98697AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P08246 | Primary Antibody | Neutrophil elastase | Human | Sheep | IgG | Polyclonal | LSBio | LS-B4244-50 | Unconjugated | AB_10912151 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P12036 | Primary Antibody | NF-H | Human | Mouse | IgG1 | SMI32 | BioLegend | 801705 | AF488 | AB_2715876 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | NF-H/NF-M | Human | Mouse | IgG1 | SMI310 | BioLegend | 837711 | AF488 | AB_2750293 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07196 | Primary Antibody | NF-L | Human | Mouse | IgG1 | NFL3 | BioLegend | 845907 | AF647 | AB_2728564 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P30548 | Primary Antibody | NK1.1 | Mouse | Mouse | IgG2a | PK136 | BioLegend | 108731 | BV421 | AB_10895916 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P30548 | Primary Antibody | NK1.1 | Mouse | Mouse | IgG2a | PK136 | BioLegend | 108731 | BV421 | AB_10895916 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8C567 | Primary Antibody | NKp46 | Mouse | Rat | IgG2a | 29A1.4 | Thermo Fisher Scientific | 12-3351-82 | PE | AB_1210743 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q92823 | Primary Antibody | NrCAM | Human | Mouse | IgG2a | N343/26 | BioLegend | 821602 | Unconjugated | AB_2734588 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| NA | Primary Antibody | Nuclear Pore Complex | Mouse | Mouse | IgG1 | MAb414 | BioLegend | 682202 | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P22736 | Primary Antibody | Nur77 | Human | Rabbit | IgG | Polyclonal | Abcam | ab48789 | Unconjugated | AB_881709 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22736 | Primary Antibody | Nur77 | Human | Mouse | IgG2b | 1E10A15 | BioLegend | 653504 | AF594 | AB_2563321 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9W6A7/Q9W6A5 | Primary Antibody | Opsin Red/Green | Zebrafish | Rabbit | IgG | Polyclonal | MilliporeSigma | AB5405 | Unconjugated | AB_177456 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P04000 | Primary Antibody | Opsin Red/Green | Mouse | Rabbit | IgG | Polyclonal | MilliporeSigma | AB5405 | Unconjugated | AB_177456 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P04000 | Primary Antibody | Opsin Red/Green | Human | Rabbit | IgG | Polyclonal | MilliporeSigma | AB5405 | Unconjugated | AB_177456 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P15863 | Primary Antibody | PAX1 | Human | Rabbit | IgG | Polyclonal | Abcam | ab203065 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q02650 | Primary Antibody | Pax5 | Mouse | Rat | IgG2a | 1H9 | BioLegend | 649704 | AF647 | AB_2562425 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 |
| A0A8C6NQH8 | Primary Antibody | PAX6 | Turquoise Killifish | Rabbit | IgG | Polyclonal | Proteintech | 12323-1-AP | Unconjugated | AB_2159695 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P55864 | Primary Antibody | PAX6 | African Clawed Frog (Xenopus laevis) | Rabbit | IgG | Polyclonal | Proteintech | 12323-1-AP | Unconjugated | AB_2159695 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P26630 | Primary Antibody | PAX6 | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 12323-1-AP | Unconjugated | AB_2159695 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P18248 | Primary Antibody | PCNA | African Clawed Frog (Xenopus laevis) | Mouse | IgG2a | PC10 | Santa Cruz Biotechnology | sc-56 | Unconjugated | AB_628110 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P18248 | Primary Antibody | PCNA | African Clawed Frog (Xenopus laevis) | Rabbit | IgG | Polyclonal | Proteintech | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q9PTP1 | Primary Antibody | PCNA | Zebrafish | Mouse | IgG2a | PC10 | Santa Cruz Biotechnology | sc-56 | Unconjugated | AB_628110 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q9PTP1 | Primary Antibody | PCNA | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Primary Antibody | PCNA | Turquoise Killifish | Rabbit | IgG | Polyclonal | Proteintech | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Primary Antibody | PCNA | Turquoise Killifish | Mouse | IgG2a | PC10 | Santa Cruz Biotechnology | sc-56 | Unconjugated | AB_628110 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P48539 | Primary Antibody | PCP4 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP1-80929 | Unconjugated | AB_11026568 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329910 | AF647 | AB_940471 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15116 | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15116 | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q15116 | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | Abcam | ab275126 | AF555 | AB_2728811 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0009-0006-9784-2694 | NA | 0009-0006-9784-2694 |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329906 | PE | AB_940483 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329920 | BV421 | AB_10960742 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329906 | PE | AB_940483 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329906 | PE | AB_940483 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15116 | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | Abcam | ab275126 | AF555 | AB_2728811 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | PD-1 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | BAF1086 | Biotin | AB_355833 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329936 | AF488 | AB_2563594 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | PD-1 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | FAB7115G | AF488 | AB_10973668 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q15116 | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | Abcam | ab275126 | AF555 | AB_2728811 | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q02242 | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | BioLegend | 135206 | PE | AB_1877231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q02242 | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | BioLegend | 135218 | BV421 | AB_2561447 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329906 | PE | AB_940483 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| A0A024FCJ9 | Primary Antibody | PD-1 | Canine | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| Q15116 | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15116 | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q66HW7 | Primary Antibody | Peripherin 2 | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 18109-1-AP | Unconjugated | AB_10665364 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| A0A8C6KR86 | Primary Antibody | Peripherin 2 | Turquoise Killifish | Rabbit | IgG | Polyclonal | Proteintech | 18109-1-AP | Unconjugated | AB_10665364 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| O42583 | Primary Antibody | Peripherin 2 | African Clawed Frog (Xenopus laevis) | Rabbit | IgG | Polyclonal | Proteintech | 18109-1-AP | Unconjugated | AB_10665364 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P60709 | Cell Structure Reagent | Phalloidin | Human | NA | NA | NA | AAT Bioquest | 23122 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P19838 | Primary Antibody | Phospho NF-Kappa B p105 | Human | Rabbit | IgG | 178F3 | Cell Signaling Technology | PA5-79525 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31751 | Primary Antibody | Phospho-Akt | Human | Rabbit | IgG | D9E | Cell Signaling Technology | 4060T | Unconjugated | AB_2315049 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P49840 | Primary Antibody | Phospho-GSK-3alpha | Human | Rabbit | IgG | 36E9 | Cell Signaling Technology | 9316 | Unconjugated | AB_659836 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q04206 | Primary Antibody | Phospho-NF-KappaB p65 (Ser536) | Human | Rabbit | IgG | 93H1 | Cell Signaling Technology | 3033 | Unconjugated | AB_331284 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| NA | Primary Antibody | Phospho-SMAD1/5 | Human | Rabbit | IgG | 41D10 | Cell Signaling Technology | 9516 | Unconjugated | AB_491015 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 |
| NA | Primary Antibody | Phospho-SMAD3 | Human | Rabbit | IgG | EP823Y | Abcam | ab52903 | Unconjugated | AB_882596 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 |
| NA | Primary Antibody | Phospho-TAK1 | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-99340 | Unconjugated | AB_2818273 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 |
| O00329 | Primary Antibody | PI3 Kinase p110 delta | Human | Rabbit | IgG | E2T2N | Cell Signaling Technology | 19591S | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01833 | Primary Antibody | PIGR | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-35340 | Unconjugated | AB_2552650 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P01833 | Primary Antibody | PIGR | Human | Rat | IgG1 | 7C1 | Abcam | ab170321 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P01833 | Primary Antibody | PIGR | Human | Rabbit | IgG | Polyclonal | Abcam | ab96196 | Unconjugated | AB_10677612 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P01833 | Primary Antibody | PIGR | Human | Rat | IgG1 | 7C1 | Abcam | ab170321 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P17252 | Primary Antibody | PKC alpha | Human | Mouse | IgG1 | H-7 | Santa Cruz Biotechnology | sc-8393 AF680 | AF680 | AB_628142 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P20444 | Primary Antibody | PKC alpha | Mouse | Mouse | IgG1 | H-7 | Santa Cruz Biotechnology | sc-8393 AF680 | AF680 | AB_628142 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| NA | Lectin | PNA | Human | NA | NA | NA | Thermo Fisher Scientific | L21409 | AF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| NA | Lectin | PNA | Zebrafish | NA | NA | NA | Thermo Fisher Scientific | L21409 | AF488 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Primary Antibody | PNL-2 | Canine | Mouse | IgG1 | PNL2 | Thermo Fisher Scientific | MSM1-894-P0 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 |
| Q9NP85 | Primary Antibody | Podocin | Human | Rabbit | IgG | JB51-33 | Novus Biologicals | NBP2-75624 | Unconjugated | AB_2892752 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86YL7 | Primary Antibody | Podoplanin | Human | Rat | IgG2a | NC-08 | BioLegend | 337018 | AF594 | AB_2629590 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86YL7 | Primary Antibody | Podoplanin | Human | Mouse | IgG1 | D2-40 | BioLegend | 916601 | Unconjugated | AB_2565182 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 |
| Q9UKI9 | Primary Antibody | POU2F3 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP1-83966 | Unconjugated | AB_11024500 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P02545 | Primary Antibody | Progerin | Human | Mouse | IgG1 | 13A4 | Thermo Fisher Scientific | 39965 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| Q7SY24 | Primary Antibody | Protein kinase C beta | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 12919-1-AP | Unconjugated | AB_2237469 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P42225 | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | Cell Signaling Technology | 9167 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| P42225 | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | Cell Signaling Technology | 9167 | Unconjugated | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | Akoya Antibody Diluent | NA | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| P42225 | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | Cell Signaling Technology | 9167 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| P42225 | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | Cell Signaling Technology | 9167 | Unconjugated | NA | Stock | IBEX2D Manual | 2% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| P42229 | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | C71E5 | Cell Signaling Technology | 9314 | Unconjugated | AB_2302702 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P42229 | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | C11C5 | Cell Signaling Technology | 9359 | Unconjugated | AB_823649 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P42229 | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | D47E7 | Cell Signaling Technology | 4322 | Unconjugated | AB_10544692 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | MilliporeSigma | SAB4600110 | CF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16720 | iF750 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16712 | iF680 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16833 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16710 | iF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11034 | AF488 | AB_2576217 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Vector Laboratories | TI-10000 | Texas Red | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25305 | AF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16678 | iF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Abcam | ab155728 | AF750 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21428 | AF555 | AB_2535849 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21245 | AF647 | AB_2535813 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21039 | AF750 | AB_2535710 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25302 | AF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Proteintech | CL594-66467 | CL594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21109 | AF680 | AB_2535758 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25303 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Mouse | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25307 | AF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21074 | AF660 | AB_2535735 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25308 | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16686 | iF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16690 | iF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21206 | AF488 | AB_2535792 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Mouse | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25303 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31572 | AF555 | AB_162543 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11009 | AF532 | AB_2534076 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21206 | AF488 | AB_2535792 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11010 | AF546 | AB_2534077 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11009 | AF532 | AB_2534076 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21207 | AF594 | AB_141637 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11037 | AF594 | AB_2534095 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21206 | AF488 | AB_2535792 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31572 | AF555 | AB_162543 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-10043 | AF680 | AB_2534018 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31573 | AF647 | AB_2536183 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31572 | AF555 | AB_162543 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-10040 | AF546 | AB_2534016 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Zebrafish | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-10042 | AF546 | AB_2534017 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21245 | AF647 | AB_2535813 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11374 | AF790 | AB_2534145 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11009 | AF532 | AB_2534076 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1118-7432 | NA | 0000-0003-1118-7432 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21207 | AF594 | AB_141637 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10031 | DL755 | AB_2556611 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-48270 | AF555 (Plus) | AB_2896336 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21247 | AF647 | AB_141778 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-625-150 | AF680 | AB_2340698 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-585-150 | AF594 | AB_2340688 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-48269 | AF488 (Plus) | AB_2893137 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-48272 | AF647 (Plus) | AB_2893138 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-545-150 | AF488 | AB_2340683 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-605-153 | AF647 | AB_2340694 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-605-153 | AF647 | AB_2340694 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21094 | AF633 | AB_2535749 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21096 | AF680 | AB_2535750 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-605-153 | AF647 | AB_2340694 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11007 | AF594 | AB_10561522 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q6PBW5 | Primary Antibody | Retinal pigment epithelium-specific protein 65kDa | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 17939-1-AP | Unconjugated | AB_2285290 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q6P027 | Primary Antibody | Retinaldehyde binding protein 1 | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 15356-1-AP | Unconjugated | AB_2178530 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P35359 | Primary Antibody | Rhodopsin | Zebrafish | Mouse | IgG1 | 1D4 | Santa Cruz Biotechnology | sc-57432 | Unconjugated | AB_785511 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q9HAT0 | Primary Antibody | ROPN1 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP1-89839 | Unconjugated | AB_11013452 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51449 | Primary Antibody | RORgamma t | Human | Mouse | IgG2a | 6F3.1 | MilliporeSigma | MABF81 | Unconjugated | AB_11205416 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | SARS-NP | Rhesus Macaque | Rabbit | IgG | Polyclonal | Novus Biologicals | NB100-56576 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lung | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| O09126 | Primary Antibody | SEMA4D | Mouse | Rat | IgG2a | BMA-12 | BioLegend | 147609 | AF594 | AB_2687303 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21448 | AF647 | AB_2535865 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21436 | AF555 | AB_2535857 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11015 | AF488 | AB_2534082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21102 | AF680 | AB_2535755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21102 | AF680 | AB_2535755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21102 | AF680 | AB_2535755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q920G3 | Primary Antibody | SiglecF | Mouse | Rat | IgG2a | E50-2440 | BD Biosciences | 552126 | PE | AB_394341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 |
| Q920G3 | Primary Antibody | SiglecF | Mouse | Rat | IgG2a | 1RNM44N | Thermo Fisher Scientific | 56-1702-80 | AF700 | AB_2637126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q920G3 | Primary Antibody | SiglecF | Mouse | Rat | IgG2a | E50-2440 | BD Biosciences | 552126 | PE | AB_394341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P97797 | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | BioLegend | 144024 | AF488 | AB_2650815 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P97797 | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | BioLegend | 144024 | AF488 | AB_2650815 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P97797 | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | BioLegend | 144027 | AF647 | AB_2721300 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P97797 | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | BioLegend | 144027 | AF647 | AB_2721300 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| Q15796 | Primary Antibody | SMAD2 | Human | Mouse | IgG1 | L16D3 | Cell Signaling Technology | 3103 | Unconjugated | AB_490816 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 |
| P84022 | Primary Antibody | SMAD3 | Human | Rabbit | IgG | Polyclonal | Abcam | ab28379 | Unconjugated | AB_2192903 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 |
| P05023 | Primary Antibody | Sodium Potassium ATPase | Human | Rabbit | IgG | EP1845Y | Abcam | ab197713 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05023 | Primary Antibody | Sodium Potassium ATPase | Human | Rabbit | IgG | EP1845Y | Abcam | ab198367 | AF647 | AB_2916056 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| Q06945 | Primary Antibody | SOX4 | Human | Mouse | IgG2b | SOX4/2540 | Novus Biologicals | NBP2-79903AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| Q06945 | Primary Antibody | SOX4 | Human | Mouse | IgG2b | CL5634 | Novus Biologicals | NBP2-61420 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| Q15506 | Primary Antibody | SPA17 | Human | Rabbit | IgG | Polyclonal | Proteintech | 13367-1-AP | Unconjugated | AB_2194631 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P09486 | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | R&D Systems | AF941 (Unconjugated) | AF532 | AB_2892754 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P09486 | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | R&D Systems | AF941 (Unconjugated) | AF532 | AB_2892754 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P09486 | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | R&D Systems | AF941 | Unconjugated | AB_355728 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P09486 | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | R&D Systems | AF941 (Unconjugated) | AF532 | AB_2892754 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P14719 | Primary Antibody | ST2 (IL-33R) | Mouse | Rat | IgG2a | U29-93 | BD Biosciences | 566311 | PE | AB_2744490 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P18827 | Primary Antibody | Syndecan-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2780 (Unconjugated) | iF594 | AB_2892765 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| O43318 | Primary Antibody | TAK1 | Human | Rabbit | IgG | Polyclonal | Proteintech | 12330-2-AP | Unconjugated | AB_2140101 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 |
| NA | Primary Antibody | TCR gamma delta | Human | Mouse | IgG1 | B1 | BioLegend | 331210 | PE | AB_1089218 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | TCR gamma delta | Human | Mouse | IgG1 | B1 | BioLegend | 331210 | PE | AB_1089218 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | BioLegend | 118128 | AF488 | AB_2562771 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | BioLegend | 118108 | PE | AB_313832 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | BioLegend | 118108 | PE | AB_313832 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | TCR gamma delta | Human | Mouse | IgG1 | B1 | BioLegend | 331225 | PE/Dazzle AF594 | AB_2565533 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | TCR Valpha24 | Human | Mouse | IgG1 | 6B11 | BioLegend | 342919 | PE/Dazzle AF594 | AB_2566311 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | TCR Valpha7.2 | Human | Mouse | IgG1 | 3C10 | BioLegend | 351706 | PE | AB_10899577 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | TCR Valpha7.2 | Human | Mouse | IgG1 | 3C10 | BioLegend | 351726 | AF647 | AB_2566335 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | TCR Valpha7.2 | Human | Mouse | IgG1 | 3C10 | BioLegend | 351729 | PE/Dazzle AF594 | AB_2566338 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9UIF3 | Primary Antibody | TEKT2 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP1-86955 | Unconjugated | AB_11017402 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P37173 | Primary Antibody | TGFBR2 | Human | Mouse | IgG1 | MM0056-4F14 | Abcam | ab78419 | Unconjugated | AB_1603198 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 |
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rabbit | IgG | Polyclonal | Abcam | ab185703 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | R&D Systems | MAB2365 | Unconjugated | AB_2232900 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | R&D Systems | MAB2365 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | R&D Systems | MAB2365 | Unconjugated | AB_2232900 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q6U7R4 | Primary Antibody | Tim-4 | Mouse | Rat | IgG1 | 21H12 | BD Biosciences | 742773 | BV421 | AB_2741037 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| O15393 | Primary Antibody | TMPRSS2 | Human | Rabbit | IgG | EPR3861 | Abcam | ab92323 | Unconjugated | AB_10585592 | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| O15393 | Primary Antibody | TMPRSS2 | Human | Rabbit | IgG | EPR3861 | Abcam | ab92323 (Unconjugated) | AF647 | AB_2892763 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15661 | Primary Antibody | Tryptase | Human | Mouse | IgG1 | AA1 | Abcam | ab2378 | Unconjugated | AB_303023 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | TUJ1 | BioLegend | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | AA10 | BioLegend | 657412 | BV421 | AB_2632699 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | TUJ1 | BioLegend | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | TUJ1 | BioLegend | 801202 (Unconjugated) | AF532 | AB_2892767 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q9ERD7 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | BioLegend | 657412 | BV421 | AB_2632699 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9ERD7 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | BioLegend | 657402 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | TUJ1 | BioLegend | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| Q9ERD7 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | BioLegend | 657402 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q4QRB4 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | TUJ1 | BioLegend | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P09936 | Primary Antibody | UCHL1 | Human | Rabbit | IgG | Polyclonal | Proteintech | 14730-1-AP | Unconjugated | AB_2210497 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 |
| P07911 | Primary Antibody | Uromodulin | Human | Mouse | IgG2b | 10.32 | Novus Biologicals | NBP1-50431 | FITC | AB_10012622 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | UT014 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL550 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | UT014 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL550 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | UT015 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL490 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | UT015 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL490 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | UT016 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL650 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | UT019 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL594 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9W6A9 | Primary Antibody | UV opsin | Zebrafish | Rabbit | IgG | Polyclonal | Kerafast | EJH013 | Unconjugated | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P33151 | Primary Antibody | VE-Cadherin | Human | Mouse | IgG2a | BV9 | BioLegend | 348512 | AF594 | AB_2563354 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20152 | Primary Antibody | Vimentin | Mouse | Rat | IgG2a | W16220A | BioLegend | 699305 | AF488 | AB_2888889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677802 (Unconjugated) | AF532 | AB_2892753 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677802 (Unconjugated) | AF532 | AB_2892753 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20152 | Primary Antibody | Vimentin | Mouse | Rat | IgG2a | W16220A | BioLegend | 699308 | AF647 | AB_2888890 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677802 (Unconjugated) | BL759/780 | NA | Custom | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20152 | Primary Antibody | Vimentin | Mouse | Rat | IgG2a | W16220A | BioLegend | 699305 | AF488 | AB_2888889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677802 (Unconjugated) | BL759/780 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P04275 | Primary Antibody | VWF | Human | Rabbit | IgG | D8L8G | Cell Signaling Technology | 65707 | Unconjugated | AB_2799692 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 |
| NA | Primary Antibody | ZO-1 | Zebrafish | Mouse | IgG1 | ZO1-1A12 | Thermo Fisher Scientific | 33-9100 | Unconjugated | AB_2533147 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q6DH07 | Primary Antibody | Zpr-1 | Zebrafish | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-43 | Unconjugated | AB_10013803 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Primary Antibody | Zpr-1 | Turquoise Killifish | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-43 | Unconjugated | AB_10013803 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Primary Antibody | Zpr-3 | African Clawed Frog (Xenopus laevis) | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-45 | Unconjugated | AB_10013805 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| P35359 | Primary Antibody | Zpr-3 | Zebrafish | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-45 | Unconjugated | AB_10013805 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| Q58EE9 | Primary Antibody | Zrf-1 | Zebrafish | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-46 | Unconjugated | AB_10013806 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |
| NA | Primary Antibody | Zrf-1 | Turquoise Killifish | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-46 | Unconjugated | AB_10013806 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 |