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Additional information on fluorescent probes tested by the IBEX Imaging Community can be found here. Details on reagent validation can be found here.
| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q9BYF1 | Primary Antibody | ACE2 | Human | Goat | IgG | Polyclonal | R&D Systems | AF933 (Unconjugated) | AF555 | AB_2892761 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9GZX7 | Primary Antibody | AID | Human | Mouse | IgG1 | 39-2500 | Thermo Fisher Scientific | ZA001 | Unconjugated | AB_2533403 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9GZX7 | Primary Antibody | AID | Human | Rat | IgG2a | mAID-2 | Thermo Fisher Scientific | 14-5959-82 | Unconjugated | AB_10669583 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Novus Biologicals | IC1420S-025 | AF750 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Abcam | ab184675 | AF488 | AB_2832195 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | Novus Biologicals | NBP2-34522IR | DL755 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | Novus Biologicals | NBP2-34522IR | DL755 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P25054 | Secondary Antibody | APC | Human | Mouse | IgG2b | 936809 | R&D Systems | FAB8927R-100UG | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29972 | Primary Antibody | Aquaporin 1 | Human | Rabbit | IgG | EPR11588(B) | Abcam | ab225225 | AF647 | AB_2810992 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P41181 | Primary Antibody | Aquaporin 2 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-33472 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41181 | Primary Antibody | Aquaporin 2 | Human | Goat | IgG | Polyclonal | Novus Biologicals | NBP1-70378 | Unconjugated | AB_11011225 | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P00966 | Primary Antibody | ASS1 | Human | Rabbit | IgG | EPR12398 | Abcam | ab170952 | Unconjugated | AB_2892766 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103248 | BV510 | AB_2650679 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 41-0452-80 | eF570 | AB_2573598 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103251 | BV421 | AB_2562905 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 42-0452-82 | eF615 | AB_10852702 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 58-0452-82 | AF532 | AB_11218492 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103232 | AF700 | AB_493717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 557683 | AF647 | AB_396793 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 557669 | AF488 | AB_396781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658703 | AF488 | AB_2563151 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658707 | PE | AB_2563281 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658705 | AF647 | AB_2563279 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658707 | PE | AB_2563281 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41182 | Primary Antibody | BCL6 | Human | Mouse | IgG1 | K112-91 | BD Biosciences | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41182 | Primary Antibody | BCL6 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-59786 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41183 | Primary Antibody | BCL6 | Mouse | Mouse | IgG1 | K112-91 | BD Biosciences | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41182 | Primary Antibody | BCL6 | Human | Mouse | IgG1 | GI191E | Thermo Fisher Scientific | 14-9887-82 | Unconjugated | AB_10669166 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P41182 | Primary Antibody | BCL6 | Human | Mouse | IgG1 | K112-91 | BD Biosciences | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P35226 | Primary Antibody | BMI-1 | Human | Mouse | IgG2b | LLBmi1-1 | Novus Biologicals | NBP1-96140AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01106 | Primary Antibody | c-Myc | Human | Mouse | IgG1 | 9.00E+10 | Novus Biologicals | NB600-302IR | DL755 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22676 | Primary Antibody | Calretinin | Mouse | Mouse | IgG1 | 6B8.2 | MilliporeSigma | MAB1568 | Unconjugated | AB_94259 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P07711 | Primary Antibody | Cathepsin L | Human | Rat | IgG2a | 204101 | R&D Systems | MAB-9521 | Unconjugated | AB_2087829 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99731 | Primary Antibody | CCL19 | Human | Mouse | IgG1 | OTI2A12 | Novus Biologicals | NBP2-71278AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| O15444 | Primary Antibody | CCL25 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-49199 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P32248 | Primary Antibody | CCR7 | Human | Goat | IgG | Polyclonal | Novus Biologicals | NB100-712SS | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P32248 | Primary Antibody | CCR7 | Human | Rabbit | IgG | Y59 | Abcam | ab32527 | Unconjugated | AB_726208 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32248 | Primary Antibody | CCR7 | Human | Rabbit | IgG | Polyclonal | LSBio | LS-C210538 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P51686 | Primary Antibody | CCR9 | Human | Mouse | IgG2a | 112509 | Novus Biologicals | FAB179G-100UG | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P51686 | Primary Antibody | CCR9 | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-33407 | Unconjugated | AB_2550787 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 103924 | PE | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 103901 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | BioLegend | 312204 | PE | AB_314915 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | BioLegend | 312204 | PE | AB_314915 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | BioLegend | 312204 | PE | AB_314915 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Rat | IgG2b | 200103 | R&D Systems | MAB1126-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 1039134 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 103924 | PE | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P38570 | Primary Antibody | CD103 | Human | Rabbit | IgG | ITGAE/3904R | Novus Biologicals | NBP3-08855AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P38570 | Primary Antibody | CD103 | Human | Rabbit | IgG | ITGAE/3904R | Novus Biologicals | NBP3-07530-100ug | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17813 | Primary Antibody | CD105 (Endoglin) | Human | Mouse | IgG1 | SN6h | BioLegend | 800505 | FITC | AB_2687058 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P19320 | Primary Antibody | CD106 | Human | Mouse | IgG1 | STA | BioLegend | 305806 | PE | AB_314561 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29533 | Primary Antibody | CD106 | Mouse | Rat | IgG2a | 429 | BioLegend | 105710 | AF488 | AB_493427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P19320 | Primary Antibody | CD106 | Human | Mouse | IgG1 | STA | BioLegend | 305806 | PE | AB_314561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29533 | Primary Antibody | CD106 | Mouse | Rat | IgG2a | 429 | Thermo Fisher Scientific | 50-1061-82 | eF660 | AB_11219864 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10721 | Primary Antibody | CD117 | Human | Mouse | IgG1 | 104D2 | BioLegend | 313234 | AF488 | AB_2566221 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10721 | Primary Antibody | CD117 | Human | Mouse | IgG1 | 104D2 | BioLegend | 313203 | PE | AB_314983 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P05532 | Primary Antibody | CD117 | Mouse | Rat | IgG2b | 2B8 | BioLegend | 105827 | BV421 | AB_10898120 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9UQ88 | Primary Antibody | CD11a | Human | Mouse | IgG1 | HI111 | AAT Bioquest | 101100C0 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101208 | PE | AB_312791 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711PE | PE | AB_323678 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | Abcam | ab187537 (Unconjugated) | AF647 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | Abcam | ab187537 (Unconjugated) | AF647 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | Abcam | ab187537 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | SP330 | Abcam | ab241408 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | SP331 | Abcam | ab238794 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EPR1344 | Abcam | ab133357 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB110-89474AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101208 | PE | AB_312791 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | IgG1 | 3.9 | BioLegend | 301603 | FITC | AB_314173 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 555392 | PE | AB_395793 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | Abcam | ab216655 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 563026 | BV510 | AB_2737960 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 562561 | BV421 | AB_2737656 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117308 | PE | AB_313777 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117371 | Spark Red 718 | AB_2924457 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | Abcam | ab216655 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561355 | FITC | AB_10611872 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20702 | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | Abcam | ab216655 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117346 | AF594 | AB_2563323 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2780 | Unconjugated | AB_442186 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BD Biosciences | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356524 | AF647 | AB_2564250 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356524 | AF647 | AB_2564250 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BD Biosciences | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356517 | BV510 | AB_2562661 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BioLegend | 142526 | AF647 | AB_2566239 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BD Biosciences | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | AAT Bioquest | 113800C0 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08571 | Primary Antibody | CD14 | Human | Rabbit | IgG | EPR3653 | Abcam | ab226121 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P08571 | Primary Antibody | CD14 | Human | Mouse | IgG1 | 61D3 | Thermo Fisher Scientific | 58-0149-41 | AF532 | AB_11218093 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08571 | Primary Antibody | CD14 | Human | Rabbit | IgG | EPR3653 | Abcam | ab226121 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P33151 | Primary Antibody | CD144 (VE-Cadherin) | Human | Mouse | IgG2a | BV9 | BioLegend | 348513 | AF647 | AB_2563669 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BioLegend | 394702 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1% PFA Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BioLegend | 394702 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BioLegend | 394702 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| A0A0B4J1G0 | Primary Antibody | CD16.2 | Mouse | Armenian Hamster | IgG | 9.00E+09 | BioLegend | 149503 | PE | AB_2565810 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P08101 | Primary Antibody | CD16/32 | Mouse | Goat | IgG | Polyclonal | R&D Systems | AF1460-SP | Unconjugated | NA | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | Thermo Fisher Scientific | MA5-11458 | Unconjugated | AB_10982556 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GH1/61 | BioLegend | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | Thermo Fisher Scientific | MA5-11458 | Unconjugated | AB_10982556 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | EDHu-1 | Novus Biologicals | NB110-40686AF647 | AF647 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GH1/61 | BioLegend | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GH1/61 | BioLegend | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG2b | EPR19518 | Abcam | ab213612 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GH1/61 | BioLegend | 333628 | BV510 | AB_2650632 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GH1/61 | BioLegend | 333611 | BV421 | AB_2562462 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG2b | 6D3.2F3 | Novus Biologicals | NBP2-36495AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GH1/61 | Thermo Fisher Scientific | 12-1639-41 | PE | AB_1963571 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GH1/61 | BioLegend | 333602 (Unconjugated) | AF532 | AB_2892759 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| F7DAU3 | Primary Antibody | CD163 | Rhesus Macaque | Mouse | IgG2b | EDHu-1 | Novus Biologicals | NB110-40686AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | Thermo Fisher Scientific | MA5-11458 | Unconjugated | AB_10982556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GH1/61 | BioLegend | 333602 (Unconjugated) | AF532 | AB_2892759 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13740 | Primary Antibody | CD166 | Human | Rabbit | IgG | EPR2759 | Abcam | ab197543 | AF488 | AB_2889210 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13740 | Primary Antibody | CD166 | Human | Mouse | IgG1 | 3A6 | BioLegend | 343903 | PE | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142416 | AF594 | AB_2565620 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | Bio-Rad | MCA884F | FITC | AB_324246 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P49682 | Primary Antibody | CD183 (CXCR3) | Human | Rabbit | IgG | Polyclonal | Abcam | ab154033 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P49682 | Primary Antibody | CD183 (CXCR3) | Human | Mouse | IgG1 | G025H7 | BioLegend | 353711 | AF647 | AB_10962946 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P49682 | Primary Antibody | CD183 (CXCR3) | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-16044 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 558112 | AF488 | AB_397034 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P15391 | Primary Antibody | CD19 | Human | Mouse | IgG1 | SJ25C1 | Thermo Fisher Scientific | 58-0198-41 | AF532 | AB_11219076 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P25918 | Primary Antibody | CD19 | Mouse | Rat | IgG2a | 6D5 | BioLegend | 115552 | AF594 | AB_2563459 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331529 | AF700 | AB_2563656 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331510 | AF647 | AB_1186032 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | AAT Bioquest | 10013150 | AF555 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331522 | AF488 | AB_10720182 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331525 | BV421 | AB_10933249 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331506 | PE | AB_1088999 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331506 | PE | AB_1088999 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11609 | Primary Antibody | CD1d | Mouse | Rat | IgG2b | 1B1 | BD Biosciences | 553846 | PE | AB_2073521 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P19437 | Primary Antibody | CD20 | Mouse | Rat | IgG2b | SA275A11 | BioLegend | 150432 | AF488 | AB_2890699 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Novus Biologicals | NBP2-80486AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Novus Biologicals | NBP2-80486AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2b | 2H7 | BioLegend | 302354 | AF594 | AB_2565619 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2b | 2H7 | BioLegend | 302322 | AF700 | AB_493753 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9ES57 | Primary Antibody | CD200 | Mouse | Rat | IgG2a | OX-90 | BD Biosciences | 565547 | BV421 | AB_2739289 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22897 | Primary Antibody | CD206 | Human | Mouse | IgG2b | 685645 | R&D Systems | MAB25341 | Unconjugated | AB_10890782 | Stock | Multiplexed 2D Imaging | FFPE | Lung | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22897 | Primary Antibody | CD206 | Human | Mouse | IgG1 | 15-2 | BioLegend | 321130 | PE/Dazzle AF594 | AB_2616867 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8VBX4 | Primary Antibody | CD207 | Mouse | Rat | IgG2a | eBioL31 | Thermo Fisher Scientific | 12-2075-80 | PE | AB_763453 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab240987 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD21 | Mouse | Rat | IgG2a | 7.E.9 | BioLegend | 123413 | Pacific Blue | AB_2085159 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF488 | NA | Custom | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354909 | FITC | AB_2561574 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab240987 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | CR2/1952 | Novus Biologicals | NBP2-60733AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | 1F8 | Novus Biologicals | NBP1-22527 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354903 | PE | AB_2561406 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P20693 | Primary Antibody | CD23 | Mouse | Rat | IgG2a | B3B4 | BioLegend | 101611 | AF647 | AB_493479 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338521 | BV421 | AB_2562460 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338505 | FITC | AB_1501125 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01589 | Primary Antibody | CD25 | Human | Mouse | IgG1 | M-A251 | BioLegend | 356128 | AF647 | AB_2563588 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01589 | Primary Antibody | CD25 | Human | Mouse | IgG2b | CD25-4E3 | Thermo Fisher Scientific | 17-0257-42 | APC | AB_11218671 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01590 | Primary Antibody | CD25 | Mouse | Rat | IgG1 | PC61.5 | Thermo Fisher Scientific | 53-0251-82 | AF488 | AB_763472 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01589 | Primary Antibody | CD25 | Human | Mouse | IgG1 | M-A251 | BioLegend | 356128 | AF647 | AB_2563588 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08138 | Primary Antibody | CD271 (NGFR5) | Human | Mouse | IgG1 | NGFR5 | Thermo Fisher Scientific | MA5-13311 | Biotin | AB_10982271 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08138 | Primary Antibody | CD271 (NGFR5) | Human | Mouse | IgG1 | NGRF5 | BioLegend | 818201 | Unconjugated | AB_2564818 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9EP73 | Primary Antibody | CD274 (PD-L1) | Mouse | Rat | IgG2b | MIH1 | BD Biosciences | 753201 | RY586 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P09055 | Primary Antibody | CD29 | Mouse | Armenian Hamster | IgG | HMB1-1 | BioLegend | 102214 | AF647 | AB_492831 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300435 | BV570 | AB_10898117 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300401 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300415 | AF488 | AB_389310 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100214 | Pacific Blue | AB_493645 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100205 | PE | AB_312662 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100234 | BV510 | AB_2562555 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100228 | BV421 | AB_2562553 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | Thermo Fisher Scientific | 58-0032-80 | AF532 | AB_11220474 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300416 | AF647 | AB_389332 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300402 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300408 | PE | AB_314062 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300415 | AF488 | AB_389310 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EPR4426 | Abcam | ab208514 | AF555 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300448 | BV510 | AB_2563468 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Thermo Fisher Scientific | 58-0038-42 | AF532 | AB_11218675 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | AAT Bioquest | 10032060 | iF514 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300487 | Spark Blue 574 | AB_2904329 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300483 | Spark Violet 538 | AB_2890733 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | CAL57 | Cell IDx | HI06B-005 | UT016 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| G7NCB9 | Primary Antibody | CD3 | Rhesus Macaque | Mouse | IgG1 | F7.2.38 | Agilent | M7254 | Unconjugated | AB_2631163 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Skin | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100281 | Spark Red 718 | AB_2924440 | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100281 | Spark Red 718 | AB_2924440 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Skin | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Novus Biologicals | NB100-2726IR | DL755 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | Novus Biologicals | NBP2-47785AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| A0A5F7Z8X0 | Primary Antibody | CD31 | Rhesus Macaque | Rabbit | IgG | Polyclonal | Abcam | ab28364 | Unconjugated | AB_726362 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303133 | AF700 | AB_2566326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303133 | AF700 | AB_2566326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102516 | AF647 | AB_2161029 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303126 | AF594 | AB_2563303 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | Novus Biologicals | NBP2-47785AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | Novus Biologicals | NBP2-47785AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102514 | AF488 | AB_2161031 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303112 | AF647 | AB_493076 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303110 | AF488 | AB_493074 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303102 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Novus Biologicals | FAB7227T | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Novus Biologicals | FAB7227T | AF594 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333404 | FITC | AB_2085022 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333406 | PE | AB_2292231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333406 | PE | AB_2292231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333406 | PE | AB_2292231 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BD Biosciences | 565329 | AF647 | AB_2739186 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56528 | Primary Antibody | CD38 | Mouse | Rat | IgG2a | 90 | BioLegend | 102732 | BV421 | AB_2734153 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P28907 | Primary Antibody | CD38 | Human | Mouse | IgG1 | HIT2 | BioLegend | 303503 | FITC | AB_314355 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P28907 | Primary Antibody | CD38 | Human | Mouse | IgG1 | HIT2 | BioLegend | 303524 | AF700 | AB_2072781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P55772 | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | BioLegend | 328205 | FITC | AB_940423 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P55772 | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | BioLegend | 328208 | PE | AB_940429 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P55772 | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | BioLegend | 328205 | FITC | AB_940423 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P22646 | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | Bio-Rad | MCA463SBB700 | StarBright Blue 700 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P22646 | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | Bio-Rad | MCA463SBV710 | StarBright Violet 710 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P22646 | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | Bio-Rad | MCA463SBV670 | StarBright Violet 670 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA1211 | Miltenyi Biotec | 130-123-209 | VioGreen | AB_2811462 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | Thermo Fisher Scientific | 58-0049-42 | AF532 | AB_2802361 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-118-568 | VioBlue | AB_2733943 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA1211 | Miltenyi Biotec | 130-128-234 | Vio Bright R720 | AB_2905066 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-118-794 | PerCP-Vio 700 | AB_2733032 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-118-316 | PE-Vio 615 | AB_2728077 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-123-894 | PE-Vio 770 | AB_2811571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-119-132 | APC-Vio 770 | AB_2733118 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Cell IDx | HI06B-005 | UT019 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | Novus Biologicals | NBP2-27216AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | Thermo Fisher Scientific | 58-0049-42 | AF532 | AB_2802361 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | AF-379-SP | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | AAT Bioquest | 10041090 | iF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BD Biosciences | 557667 | AF488 | AB_396779 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100443 | BV421 | AB_2562557 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 58-0042-80 | AF532 | AB_11219484 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16003 | Primary Antibody | CD4 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | FAB8165N | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300526 | AF700 | AB_493743 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 58-0042-80 | AF532 | AB_11219484 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 41-0042-82 | eF570 | AB_2573595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16003 | Primary Antibody | CD4 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300519 | AF488 | AB_389311 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BD Biosciences | 553730 | PE | AB_396634 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | GK1.5 | BioLegend | 100446 | AF594 | AB_2563182 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | AF-379-SP | Unconjugated | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103026 | AF700 | AB_493713 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103074 | Spark YG 570 | AB_2892265 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103007 | PE | AB_312958 | Stock | Multiplexed 2D Imaging | FFPE | Thymus | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103054 | AF594 | AB_2563183 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | BioLegend | 304018 | AF647 | AB_389336 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016184 | PE/iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016184 | PE/iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103122 | AF488 | AB_493531 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Novus Biologicals | NB100-77417IR | DL755 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-9189-7172 | NA | 0000-0001-9189-7172 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103122 | AF488 | AB_493531 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Uterus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2634-6838 | NA | 0000-0003-2634-6838 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528PE | PE | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103124 | AF647 | AB_493533 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | Thermo Fisher Scientific | 58-0459-41 | AF532 | AB_11218084 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Infected | Akoya Antibody Diluent | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-9189-7172 | NA | 0000-0001-9189-7172 |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | BioLegend | 304060 | AF594 | AB_2629599 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103134 | BV421 | AB_2562559 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103134 | BV421 | AB_2562559 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103138 | BV510 | AB_2563061 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | CD45RA | Human | Mouse | IgG1 | 5H9 | BD Biosciences | 556626 | FITC | AB_396498 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328303 | PE | AB_1236407 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | CL7207 | Abcam | ab243032 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | CL7207 | Abcam | ab243032 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328308 | FITC | AB_2129084 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353125 | AF700 | AB_2810560 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | BioLegend | 304611 | AF488 | AB_493179 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | BioLegend | 304603 | FITC | AB_314445 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | BioLegend | 304605 | PE | AB_314447 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Ileum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3.D5 | Novus Biologicals | NBP2-33132AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3.D5 | Novus Biologicals | NBP2-33132AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3 | Bio-Rad | MCA2693A488T | AF488 | AB_1102209 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| F7DRX3 | Primary Antibody | CD57 | Human | Mouse | IgM | HNK-1 | BioLegend | 359602 | Unconjugated | AB_2562403 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| F7DRX3 | Primary Antibody | CD57 | Human | Mouse | IgM | HNK-1 | BioLegend | 359602 | Unconjugated | AB_2562403 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05106 | Primary Antibody | CD61 | Human | Mouse | IgG1 | Y2/51 | Miltenyi Biotec | 130-098-683 | FITC | AB_2660493 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139304 | PE | AB_10612740 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139322 | AF647 | AB_2566561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13688 | Primary Antibody | CD66abce | Rhesus Macaque | Mouse | IgG2b | Tet2 | Miltenyi Biotec | 130-116-522 | FITC | AB_2727588 | Stock | Opal-plex | 10% Formalin for 7 Days | Lung | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| P31997 | Primary Antibody | CD66b | Human | Mouse | IgM | G10F5 | Caprico Biotechnologies | 1075136 | iF594 | AB_2892744 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31997 | Primary Antibody | CD66b | Human | Mouse | IgM | G10F5 | BioLegend | 305110 | AF647 | AB_2563171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Uterus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2634-6838 | NA | 0000-0003-2634-6838 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137011 | AF488 | AB_2074847 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 | AF647 | AB_2891106 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 AF647 | AF647 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 | AF647 | AB_2891106 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | BioLegend | 310918 | AF647 | AB_528871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | BioLegend | 310906 | PE | AB_314841 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3218-4479 | NA | 0000-0002-3218-4479 |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | BioLegend | 310918 | AF647 | AB_528871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG2a | 15B5G2 | Novus Biologicals | NBP2-25236AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q61503 | Primary Antibody | CD73 | Mouse | Rat | IgG1 | TY/11.8 | BioLegend | 127230 | AF700 | AB_2800625 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-2634-6838 | NA | 0000-0002-1461-0999 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Skin | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100758 | AF594 | AB_2563237 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100752 | BV510 | AB_2563057 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100723 | AF488 | AB_389304 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BD Biosciences | 553032 | PE | AB_394571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Heart | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Rabbit | IgG | EPR10640 | Cell IDx | HI06B-005 | UT015 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372910 | Spark YG 570 | AB_2904405 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | Thermo Fisher Scientific | 14-0085-82 | Unconjugated | AB_11150240 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100724 | AF647 | AB_389326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Skin | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | RPA-T8 | Thermo Fisher Scientific | 58-0088-24 | AF532 | AB_11220473 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | RPA-T8 | BD Biosciences | 557945 | AF700 | AB_396953 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | AAT Bioquest | 100810C0 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Heart | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 |
| P33681 | Primary Antibody | CD80 | Human | Mouse | IgG1 | 2D10 | BioLegend | 305215 | AF647 | AB_528874 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P33681 | Primary Antibody | CD80 | Human | Goat | IgG | Polyclonal | R&D Systems | AF140 | Unconjugated | AB_354778 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P33681 | Primary Antibody | CD80 | Human | Mouse | IgG2b | 62N3G8 | Novus Biologicals | NBP2-25255AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q01151 | Primary Antibody | CD83 | Human | Mouse | IgG1 | HB15e | BD Biosciences | 550634 | PE | AB_393794 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q01151 | Primary Antibody | CD83 | Human | Mouse | IgG1 | HB15e | BioLegend | 305314 | AF488 | AB_528877 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P42081 | Primary Antibody | CD86 | Human | Mouse | IgG2b | IT2.2 | BioLegend | 305415 | AF647 | AB_528882 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P42081 | Primary Antibody | CD86 | Human | Goat | IgG | Polyclonal | R&D Systems | AF-141-NA | Unconjugated | AB_354385 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P42082 | Primary Antibody | CD86 (B7-2) | Mouse | Rat | IgG2a | GL1 | R&D Systems | FAB741S-100UG | AF750 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P01731 | Primary Antibody | CD8a | Mouse | Rat | IgG2a | 53-6.7 | R&D Systems | FAB116S-100UG | AF750 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X |
| P01732 | Primary Antibody | CD8a | Mouse | Mouse | IgG1 | HIT8a | BioLegend | 300908 | PE | AB_314112 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P01831 | Primary Antibody | CD90.1 | Mouse | Mouse | IgG1 | OX-7 | BioLegend | 202508 | AF647 | AB_492884 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305512 | AF647 | AB_2249691 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305506 | PE | AB_314536 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305506 | PE | AB_314536 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305504 | FITC | AB_314534 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305506 | AF488 | AB_314536 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NXV6 | Primary Antibody | CDKN2a | Human | Rat | IgG2b | 5-C3-1 | Novus Biologicals | NB200-174AF647 | AF647 | AB_10002046 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NXV6 | Primary Antibody | CDKN2a | Human | Mouse | IgG2a | ARF 4C6/4 | Santa Cruz Biotechnology | sc-53392 | Unconjugated | AB_630056 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P13569 | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | Thermo Fisher Scientific | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P13569 | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | Thermo Fisher Scientific | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) using the Leica Bond | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P13569 | Primary Antibody | CFTR | Human | Mouse | IgG1 | M3A7 | Thermo Fisher Scientific | MA5-11768 | Unconjugated | AB_10983660 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| NA | Secondary Antibody | Chicken IgY (H&L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-32932 | AF555 | AB_2762844 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| NA | Secondary Antibody | Chicken IgY (H&L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA1-72000 | FITC | AB_923386 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10645 | Primary Antibody | Chromogranin A | Human | Mouse | IgG1 | LK2H10 | Novus Biologicals | NBP2-34672AF532 | AF532 | AB_2892553 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P58304 | Primary Antibody | Chx10 | Mouse | Mouse | IgG2a | E-12 | Santa Cruz Biotechnology | sc-365519 AF546 | AF546 | AB_10842442 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P70194 | Primary Antibody | CLEC4F | Mouse | Goat | IgG | Polyclonal | R&D Systems | AF2784 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| Q6UXN8 | Primary Antibody | CLEC9a | Human | Mouse | IgG2a | 8F9 | BioLegend | 353802 (Unconjugated) | AF488 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BWP8 | Primary Antibody | COLEC11 | Human | Rabbit | IgG | Polyclonal | Proteintech | 15269-1-AP | Unconjugated | AB_10732940 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P02462 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02462 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02462 | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | Abcam | ab6586 | Unconjugated | AB_305584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02462 | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | Abcam | ab6586 | NA | AB_305584 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02462 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02462 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9EQP6 | Primary Antibody | Cone Arrestin | Mouse | Rabbit | IgG | Polyclonal | MilliporeSigma | AB15282 | Unconjugated | AB_1163387 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P16410 | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | Abcam | ab270127 | AF647 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16410 | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | Abcam | ab270127 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q6ICW0 | Primary Antibody | CXCL12 | Human | Mouse | IgG1 | 79018 | R&D Systems | MAB350 | Unconjugated | AB_2088149 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q6ICW0 | Primary Antibody | CXCL12 | Human | Mouse | IgG1 | 79018 | R&D Systems | MAB350-500 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 | Unconjugated | AB_2892755 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 | Unconjugated | AB_2892755 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 (Unconjugated) | AF532 | AB_2892755 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 (Unconjugated) | AF532 | AB_2892755 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9EQ16 | Primary Antibody | CXCR6 | Mouse | Rat | IgG2b | FAB2145R | Novus Biologicals | 221002 | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| NA | Primary Antibody | Cytokeratin | Mouse | Mouse | IgG1 | C-11 | BioLegend | 628604 | AF647 | AB_2563652 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Novus Biologicals | NBP2-33200AF750 | AF750 | AB_2868569 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 42-9003-82 | eF615 | AB_10804663 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 50-9003-82 | eF660 | AB_2574301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 41-9003-80 | eF570 | AB_11217482 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | BioLegend | 914204 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 50-9003-82 | eF660 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P13645 | Primary Antibody | Cytokeratin 10 | Human | Mouse | IgG1 | DE-K10 | Novus Biologicals | NBP2-54402AF488 | AF488 | AB_2892746 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P02533 | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 | Unconjugated | AB_2616962 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P02533 | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 | Unconjugated | AB_2616962 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02533 | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 (Unconjugated) | AF647 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P05784 | Primary Antibody | Cytokeratin 18 | Mouse | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-14263 | Unconjugated | AB_2133178 | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| P08729 | Primary Antibody | Cytokeratin 7 | Human | Rat | IgG2a | W16155A | BioLegend | 601606 | AF488 | AB_2728458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3218-4479 | NA | 0000-0002-3218-4479 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | LSBio | LS-B3782 | Unconjugated | AB_10689801 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | LSBio | LS-B3782 | Unconjugated | AB_10689801 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | 9E9A8 | BioLegend | 330112 | AF647 | AB_1186092 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | C209/1781 | Novus Biologicals | NBP2-54351AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | 102E11.06 | Dendritics | DDX0202A647-100 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | 9E9A8 | BioLegend | 330112 | AF647 | AB_1186092 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | 102E11.06 | Dendritics | DDX0202A647-100 | AF647 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | DC28 | R&D Systems | MAB16211-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| O15075 | Primary Antibody | DCAMKL1 | Human | Rabbit | IgG | Polyclonal | Abcam | ab31704 | Unconjugated | AB_873537 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9JLM8 | Primary Antibody | DCAMKL1 | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab37994 | Unconjugated | AB_873538 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| O15075 | Primary Antibody | DCAMKL1 | Human | Rabbit | IgG | EPR6085 | Abcam | ab187184 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q60767 | Primary Antibody | DEC205 | Mouse | Rat | IgG2a | NLDC-145 | BioLegend | 138204 | AF647 | AB_2137655 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q60767 | Primary Antibody | DEC205 | Mouse | Rat | IgG2a | NLDC-145 | BioLegend | 138204 | AF647 | AB_2137655 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17661 | Primary Antibody | Desmin | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab15200 | Unconjugated | AB_301744 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P09803 | Primary Antibody | E-cadherin | Mouse | Rat | IgG1 | DECMA-1 | BioLegend | 147308 | AF647 | AB_2563955 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P32249 | Primary Antibody | EBI2 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NLS46 | Unconjugated | AB_10001402 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32249 | Primary Antibody | EBI2 | Human | Mouse | IgG2a | SA313E4 | BioLegend | 368903 | AF647 | AB_2566384 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P32249 | Primary Antibody | EBI2 | Human | Rabbit | IgG | Polyclonal | RayBiotech | 119-10961 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | EBOV NP | Mouse | Rabbit | NA | Polyclonal | IBT BioServices | 0301-012 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| NA | Primary Antibody | EBOV NP | Mouse | Rabbit | NA | Polyclonal | IBT BioServices | 0301-012 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324228 | AF594 | AB_2563209 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JW5 | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | BioLegend | 118222 | AF594 | AB_2563322 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Rabbit | IgG | Polyclonal | Abcam | ab71916 | Unconjugated | AB_1603782 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JW5 | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | BD Biosciences | 747748 | BV510 | AB_2872217 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324243 | AF700 | AB_2750488 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324235 | BV510 | AB_2632934 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JW5 | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | BioLegend | 118212 | AF647 | AB_1134101 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324212 | AF647 | AB_756086 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG1 | 1B7 | Thermo Fisher Scientific | 50-9326-42 | eF660 | AB_10598658 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324210 | AF488 | AB_756084 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324202 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123130 | AF700 | AB_2293450 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q3S4B0 | Primary Antibody | F4/80 | Mouse | Rabbit | IgG | D2S9R | Cell Signaling Technology | 70076 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| Q14246 | Primary Antibody | F4/80 | Mouse | Rat | IgG2b | F4/80 | Abcam | ab204467 | AF647 | AB_2810932 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123132 | BV421 | AB_11203717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123132 | BV421 | AB_11203717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | Thermo Fisher Scientific | 12-4801-82 | PE | AB_465923 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123122 | AF647 | AB_893480 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q3S4B0 | Primary Antibody | F4/80 | Mouse | Rabbit | IgG | D2S9R | Cell Signaling Technology | 70076 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 |
| Q14246 | Primary Antibody | F4/80 | Mouse | Rat | IgG2b | F4/80 | Abcam | ab204467 | AF647 | AB_2810932 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| NA | Blocking Reagent | Fc Block | Human | Human | IgG1 | Fc1 | BD Biosciences | 564220 | Unconjugated | AB_2869554 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Blocking Reagent | Fc Block | Mouse | Rat | IgG2b | 2.4G2 | BD Biosciences | 553141 | Unconjugated | AB_394656 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q8TBJ5 | Primary Antibody | FEZF2 | Human | Rabbit | IgG | Polyclonal | Abcam | ab214186 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P02671 | Primary Antibody | Fibrinogen | Human | Sheep | IgG | Polyclonal | Abcam | ab118533 | Unconjugated | AB_10900171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02671 | Primary Antibody | Fibrinogen | Human | Sheep | IgG | Polyclonal | Abcam | ab118533 | Unconjugated | AB_10900171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q12884 | Primary Antibody | Fibroblast activation protein alpha | Human | Rabbit | IgG | Polyclonal | MilliporeSigma | ABT11 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02751 | Primary Antibody | Fibronectin | Human | Mouse | IgG1 | 2F4 | Novus Biologicals | NBP2-22113AF532 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Fluorescein/Oregon Green | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | A-11090 | AF488 | AB_221562 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q12951 | Primary Antibody | FOXI1 | Human | Mouse | IgG1 | OTI1D4 | Novus Biologicals | NBP2-70747AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q9H334 | Primary Antibody | FOXP1 | Human | Mouse | IgG2a | JC12 | Novus Biologicals | NB100-65125 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 53-5773-82 | AF488 | AB_763537 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 12-5773-82 | PE | AB_465936 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS2 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 17-4777-42 | APC | AB_10804651 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS2 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 17-4777-42 | APC | AB_10804651 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 41-5773-82 | eF570 | AB_11219073 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 14-4777-82 | Unconjugated | AB_467556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Rabbit | IgG | 1054C | Cell IDx | HI06B-005 | UT014 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 50-4777-42 | eF660 | AB_2574219 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 53-4777-41 | AF488 | AB_10852836 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 13-4777-82 | Biotin | AB_466655 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 58-5773-82 | AF532 | AB_11218870 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 14-4777-82 | Unconjugated | AB_467556 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| NA | Primary Antibody | Francisella Tularensis, LPS | Mouse | Mouse | IgG2a | 1.B.288 | US Biological Life Sciences | F6070-02X | Unconjugated | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | NA | Yes | 0000-0001-9189-7172 | NA | 0000-0001-9189-7172 |
| Q14392 | Primary Antibody | GARP | Human | Mouse | IgG2b | 7B11 | BioLegend | 352502 | Unconjugated | AB_10897447 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q14392 | Primary Antibody | GARP | Human | Mouse | IgG2b | 7B11 | BioLegend | 352503 | PE | AB_10896792 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q14392 | Primary Antibody | GARP | Human | Mouse | IgG2b | Plato-1 | Enzo | ALX-804-867-C100 | Unconjugated | AB_2051806 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P23771 | Primary Antibody | GATA-3 | Human | Mouse | IgG1 | L50-823 | BD Biosciences | 560077 | AF488 | AB_1645303 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P14136 | Primary Antibody | GFAP | Mouse | Mouse | IgG2b | SMI 25 | BioLegend | 837504 | AF647 | AB_2632641 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| Q76IQ4 | Primary Antibody | Ghrelin | Human | Mouse | IgG2b | 891240 | R&D Systems | MAB8149 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | GL-7 | Mouse | Rat | IgM | GL7 | BD Biosciences | 561530 | PE | AB_10715834 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | GL7 | Mouse | Rat | IgM | GL7 | BD Biosciences | 561530 | PE | AB_10715834 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Rabbit | IgG | EPR13022(B) | Abcam | ab302584 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2bc | GLUL/6600 | Novus Biologicals | NBP3-14023AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Rabbit | IgG | Polyclonal | Abcam | ab49873 | Unconjugated | AB_880241 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P15104 | Primary Antibody | Glutamine synthetase | Mouse | Mouse | IgG1 | 1B6G4 | Proteintech | CL488-66323 | AF488 | AB_2883309 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2a | OTI1F4 | Novus Biologicals | NBP2-70834AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P15104 | Primary Antibody | Glutamine synthetase | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab49873 | Unconjugated | AB_880241 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 |
| P02724 | Primary Antibody | Glycophorin A | Human | Rabbit | IgG | EPR8200 | Abcam | ab218372 (Unconjugated) | AF555 | AB_2892758 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P02724 | Primary Antibody | Glycophorin A | Human | Rabbit | IgG | EPR8200 | Abcam | ab218372 (Unconjugated) | AF555 | AB_2892758 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11055 | AF488 | AB_2534102 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11055 | AF488 | AB_2534102 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Abcam | ab175745 | AF750 | AB_2924800 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21084 | AF680 | AB_2535741 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21084 | AF680 | AB_2535741 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21447 | AF647 | AB_2535864 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-32930 | AF800 (Plus) | AB_2762842 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10091 | DL755 | AB_2556671 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10091 | DL755 | AB_2556671 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11058 | AF594 | AB_2534105 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11056 | AF546 | AB_2534103 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11055 | AF488 | AB_2534102 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21084 | AF680 | AB_2535741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q62011 | Primary Antibody | gp38 | Mouse | Syrian Hamster | IgG | 8.1.1 | BioLegend | 127401 | Unconjugated | AB_1089186 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10144 | Primary Antibody | Granzyme B | Human | Rabbit | IgG | EP230 | Cell Marque | 262R-17 | Unconjugated | AB_2889344 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P10144 | Primary Antibody | Granzyme B | Human | Rabbit | IgG | Polyclonal | Abcam | ab4059 | Unconjugated | AB_304251 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| F7CLT4 | Primary Antibody | Granzyme B | Rhesus Macaque | Mouse | IgG2a | GrB-7 | Agilent | M7235 | Unconjugated | AB_2114697 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| NA | Secondary Antibody | Hamster IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21451 | AF647 | AB_2535868 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q64525 | Primary Antibody | Histone H2B | Mouse | Rat | IgG2a | W17025A | BioLegend | 606301 | Unconjugated | AB_2728465 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P02302 | Primary Antibody | Histone H3 | Mouse | Mouse | IgG3 | 1B1-B2 | BioLegend | 819406 | AF594 | AB_2715803 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| P68431 | Primary Antibody | Histone H3 (citrulline R2 + R8 + R17) | Human | Rabbit | IgG | Polyclonal | Abcam | ab5103 | Unconjugated | AB_304752 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307619 | AF488 | AB_493176 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Caprico Biotechnologies | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Abcam | ab20181 | AF647 | AB_445401 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670PE | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Caprico Biotechnologies | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307622 | AF647 | AB_493177 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670 | PE | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Caprico Biotechnologies | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Abcam | ab176408 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Abcam | ab176408 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307625 | AF700 | AB_493770 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307602 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Novus Biologicals | NB100-77855AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AF9961) and pH 9 for 30 minutes ER2 (AF9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01911 | Primary Antibody | HLA-DRB | Human | Mouse | IgG2b | SPM288 | Novus Biologicals | NBP2-45312-0.02mg | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P63158 | Primary Antibody | HMGB-1 | Mouse | Mouse | IgG2 | 3.00E+08 | BioLegend | 651406 | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X |
| Q5ZPR3 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313514 | AF488 | AB_2122584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q5ZPR3 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313527 | AF700 | AB_2566125 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q5ZPR3 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313514 | AF488 | AB_2122584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q5ZPR3 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313515 | AF647 | AB_2122583 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q5ZPR3 | Primary Antibody | ICOS | Human | Rabbit | IgG | SP98 | Abcam | ab105227 | Unconjugated | AB_10710236 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01878 | Primary Antibody | IgA | Mouse | Goat | IgG | Polyclonal | SouthernBiotech | 1040-32 | AF555 | AB_2794378 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01878 | Primary Antibody | IgA | Mouse | Goat | IgG | Polyclonal | SouthernBiotech | 1040-32 | AF555 | AB_2794378 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01876 | Primary Antibody | IgA1 | Human | Mouse | IgG1 | B3506B4 | SouthernBiotech | 9130-31 | AF647 | AB_2796658 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01876 | Primary Antibody | IgA1 | Human | Mouse | IgG1 | B3506B4 | SouthernBiotech | 9130-31 | AF647 | AB_2796658 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01877 | Primary Antibody | IgA2 | Human | Mouse | IgG1 | A9604D2 | SouthernBiotech | 9140-30 | AF488 | AB_2796665 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01877 | Primary Antibody | IgA2 | Human | Mouse | IgG1 | A9604D2 | SouthernBiotech | 9140-30 | AF488 | AB_2796665 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405718 | AF488 | AB_10730619 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 |
| P01880 | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | BioLegend | 348229 | AF700 | AB_2563334 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01880 | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | BioLegend | 348216 | AF488 | AB_11150595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| NA | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405718 | AF488 | AB_10730619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405740 | AF594 | AB_2565572 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P01880 | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | BioLegend | 348216 | AF488 | AB_11150595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| P06336 | Primary Antibody | IgE | Mouse | Rat | IgG1 | RME-1 | BioLegend | 406908 | PE | AB_493290 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P01868 | Primary Antibody | IgG1 | Mouse | Rat | IgG | RMG1-1 | BioLegend | 406616 | BV421 | AB_2562234 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 |
| P07476 | Primary Antibody | Involucrin | Human | Mouse | IgG1 | SY5 | Santa Cruz Biotechnology | sc-21748 AF488 | AF488 | AB_2128119 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| P07476 | Primary Antibody | Involucrin | Human | Rabbit | IgG | Polyclonal | Abcam | ab53112 | Unconjugated | AB_880813 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 |
| Q15306 | Primary Antibody | IRF4 | Human | Mouse | IgG1 | 4G10 | Novus Biologicals | NBP1-47396 | Unconjugated | AB_10010349 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15306 | Primary Antibody | IRF4 | Human | Rabbit | IgG | EP5699 | Abcam | ab252024 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 |
| Q15306 | Primary Antibody | IRF4 | Human | Sheep | IgG | Polyclonal | R&D Systems | AF5525 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA |