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Additional information on fluorescent probes tested by the IBEX Imaging Community can be found here. Details on reagent validation can be found here.

UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor
Q9BYF1 Primary Antibody ACE2 Human Goat IgG Polyclonal R&D Systems AF933 (Unconjugated) AF555 AB_2892761 Custom IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9GZX7 Primary Antibody AID Human Rat IgG2a mAID-2 Thermo Fisher Scientific 14-5959-82 Unconjugated AB_10669583 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9GZX7 Primary Antibody AID Human Mouse IgG1 39-2500 Thermo Fisher Scientific ZA001 Unconjugated AB_2533403 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 50-9760-82 eF660 AB_2574362 Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62737 Primary Antibody Alpha smooth muscle actin Mouse Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Novus Biologicals IC1420S-025 AF750 NA Stock IBEX2D Manual FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes No 0000-0002-5187-810X NA 0000-0002-5187-810X
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 50-9760-82 eF660 AB_2574362 Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0003-3697-5726 NA 0000-0003-3697-5726
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 41-9760-82 eF570 AB_2573631 Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0003-3697-5726 NA 0000-0003-3697-5726
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 41-9760-82 eF570 AB_2573631 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 41-9760-82 eF570 AB_2573631 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 41-9760-82 eF570 AB_2573631 Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4/asm-1 Novus Biologicals NBP2-34522IR DL755 NA Stock IBEX2D Manual FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
P62737 Primary Antibody Alpha smooth muscle actin Mouse Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual FFPE Tonsil NA 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P62737 Primary Antibody Alpha smooth muscle actin Mouse Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62737 Primary Antibody Alpha smooth muscle actin Mouse Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62737 Primary Antibody Alpha smooth muscle actin Mouse Mouse IgG2a 1A4 Abcam ab184675 AF488 AB_2832195 Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4/asm-1 Novus Biologicals NBP2-34522IR DL755 NA Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62737 Primary Antibody Alpha smooth muscle actin Mouse Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 50-9760-82 eF660 AB_2574362 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 50-9760-82 eF660 AB_2574362 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 50-9760-82 eF660 AB_2574362 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P25054 Secondary Antibody APC Human Mouse IgG2b 936809 R&D Systems FAB8927R-100UG AF647 NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P29972 Primary Antibody Aquaporin 1 Human Rabbit IgG EPR11588(B) Abcam ab225225 AF647 AB_2810992 Stock Multiplexed 2D Imaging 4% PFA Fixed Frozen Kidney NA 0.3% Triton-X-100 NA NA Yes 0000-0002-2950-2683 NA 0000-0002-2950-2683
P41181 Primary Antibody Aquaporin 2 Human Goat IgG Polyclonal Novus Biologicals NBP1-70378 Unconjugated AB_11011225 Stock Multiplexed 2D Imaging FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P41181 Primary Antibody Aquaporin 2 Human Rabbit IgG Polyclonal Novus Biologicals NBP2-33472 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P36575 Primary Antibody Arrestin C Human Mouse IgG1 7G6 MilliporeSigma MABN2636-100UG Unconjugated AB_2935804 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA NA Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
P00966 Primary Antibody ASS1 Human Rabbit IgG EPR12398 Abcam ab170952 Unconjugated AB_2892766 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA NA Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 BD Biosciences 557683 AF647 AB_396793 Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 BioLegend 103248 BV510 AB_2650679 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 BioLegend 103226 AF647 AB_389330 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 BioLegend 103251 BV421 AB_2562905 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 BD Biosciences 553090 PE AB_394619 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 BD Biosciences 553090 PE AB_394619 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 BioLegend 103232 AF700 AB_493717 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 Thermo Fisher Scientific 42-0452-82 eF615 AB_10852702 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 Thermo Fisher Scientific 41-0452-80 eF570 AB_2573598 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 Thermo Fisher Scientific 58-0452-82 AF532 AB_11218492 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 BD Biosciences 553090 PE AB_394619 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 BD Biosciences 557669 AF488 AB_396781 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967, 0000-0003-1118-7432 NA 0000-0003-4379-8967
P10415 Primary Antibody BCL2 Human Mouse IgG1 100 BioLegend 658703 AF488 AB_2563151 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10415 Primary Antibody BCL2 Human Mouse IgG1 100 BioLegend 658707 PE AB_2563281 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10415 Primary Antibody BCL2 Human Rabbit IgG SP66 Abcam ab236221 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10415 Primary Antibody BCL2 Human Rabbit IgG SP66 Abcam ab236221 Unconjugated NA Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10415 Primary Antibody BCL2 Human Mouse IgG1 100 BioLegend 658707 PE AB_2563281 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10415 Primary Antibody BCL2 Human Mouse IgG1 100 BioLegend 658705 AF647 AB_2563279 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10415 Primary Antibody BCL2 Human Rabbit IgG SP66 Abcam ab236221 Unconjugated NA Stock Cell DIVE-IBEX FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10415 Primary Antibody BCL2 Human Rabbit IgG SP66 Abcam ab236221 Unconjugated NA Stock Cell DIVE-IBEX FFPE Lymph Node NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P41182 Primary Antibody BCL6 Human Mouse IgG1 GI191E Thermo Fisher Scientific 14-9887-82 Unconjugated AB_10669166 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P41183 Primary Antibody BCL6 Mouse Mouse IgG1 K112-91 BD Biosciences 561525 AF647 AB_10898007 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P41182 Primary Antibody BCL6 Human Mouse IgG1 K112-91 BD Biosciences 561525 AF647 AB_10898007 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P41182 Primary Antibody BCL6 Human Mouse IgG1 K112-91 BD Biosciences 561525 AF647 AB_10898007 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P41182 Primary Antibody BCL6 Human Rabbit IgG Polyclonal Novus Biologicals NBP2-59786 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P35226 Primary Antibody BMI-1 Human Mouse IgG2b LLBmi1-1 Novus Biologicals NBP1-96140AF488 AF488 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01106 Primary Antibody c-Myc Human Mouse IgG1 9E10 R&D Systems MAB3696-SP Unconjugated NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01106 Primary Antibody c-Myc Human Mouse IgG1 9.00E+10 Novus Biologicals NB600-302IR DL755 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P05937 Primary Antibody Calbindin Human Rabbit IgG Polyclonal MilliporeSigma ABN2192 Unconjugated AB_2935805 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA NA Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
P22676 Primary Antibody Calretinin Mouse Mouse IgG1 6B8.2 MilliporeSigma MAB1568 Unconjugated AB_94259 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA NA Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
P07711 Primary Antibody Cathepsin L Human Rat IgG2a 204101 R&D Systems MAB-9521 Unconjugated AB_2087829 Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q99731 Primary Antibody CCL19 Human Mouse IgG1 OTI2A12 Novus Biologicals NBP2-71278AF532 AF532 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
O15444 Primary Antibody CCL25 Human Rabbit IgG Polyclonal Novus Biologicals NBP2-49199 Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
P32248 Primary Antibody CCR7 Human Goat IgG Polyclonal Novus Biologicals NB100-712SS Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
P32248 Primary Antibody CCR7 Human Rabbit IgG Polyclonal LSBio LS-C210538 Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P32248 Primary Antibody CCR7 Human Rabbit IgG Y59 Abcam ab32527 Unconjugated AB_726208 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P51686 Primary Antibody CCR9 Human Mouse IgG2a 112509 Novus Biologicals FAB179G-100UG AF488 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
P51686 Primary Antibody CCR9 Human Rabbit IgG Polyclonal Thermo Fisher Scientific PA5-33407 Unconjugated AB_2550787 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
P08473 Primary Antibody CD10 Human Goat IgG Polyclonal R&D Systems AF1182 Unconjugated AB_354652 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08473 Primary Antibody CD10 Human Goat IgG Polyclonal R&D Systems AF1182 Unconjugated AB_354652 Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08473 Primary Antibody CD10 Human Mouse IgG1 FR4D11 Caprico Biotechnologies 103924 PE NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08473 Primary Antibody CD10 Human Mouse IgG1 FR4D11 Caprico Biotechnologies 103901 Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08473 Primary Antibody CD10 Human Mouse IgG1 FR4D11 Caprico Biotechnologies 1039134 iF594 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08473 Primary Antibody CD10 Human Mouse IgG1 FR4D11 Caprico Biotechnologies 103924 PE NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08473 Primary Antibody CD10 Human Mouse IgG1 HI10a BioLegend 312204 PE AB_314915 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08473 Primary Antibody CD10 Human Goat IgG Polyclonal R&D Systems AF1182 Unconjugated AB_354652 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08473 Primary Antibody CD10 Human Mouse IgG1 HI10a BioLegend 312204 PE AB_314915 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08473 Primary Antibody CD10 Human Mouse IgG1 HI10a BioLegend 312204 PE AB_314915 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08473 Primary Antibody CD10 Human Rat IgG2b 200103 R&D Systems MAB1126-SP Unconjugated NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P38570 Primary Antibody CD103 Human Rabbit IgG ITGAE/3904R Novus Biologicals NBP3-08855AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P38570 Primary Antibody CD103 Human Rabbit IgG ITGAE/3904R Novus Biologicals NBP3-07530-100ug Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17813 Primary Antibody CD105 (Endoglin) Human Mouse IgG1 SN6h BioLegend 800505 FITC AB_2687058 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0002-3882-457X NA 0000-0002-3882-457X
P29533 Primary Antibody CD106 Mouse Rat IgG2a 429 BioLegend 105710 AF488 AB_493427 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P29533 Primary Antibody CD106 Mouse Rat IgG2a 429 Thermo Fisher Scientific 50-1061-82 eF660 AB_11219864 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P19320 Primary Antibody CD106 Human Mouse IgG1 STA BioLegend 305806 PE AB_314561 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P19320 Primary Antibody CD106 Human Mouse IgG1 STA BioLegend 305806 PE AB_314561 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P05532 Primary Antibody CD117 Mouse Rat IgG2b 2B8 BioLegend 105827 BV421 AB_10898120 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10721 Primary Antibody CD117 Human Mouse IgG1 104D2 BioLegend 313203 PE AB_314983 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0002-5187-810X NA 0000-0002-5187-810X
P10721 Primary Antibody CD117 Human Mouse IgG1 104D2 BioLegend 313234 AF488 AB_2566221 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9UQ88 Primary Antibody CD11a Human Mouse IgG1 HI111 AAT Bioquest 101100C0 iF594 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P24788 Primary Antibody CD11b Mouse Rat IgG2b M1/70 BioLegend 101217 AF488 AB_389305 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P24788 Primary Antibody CD11b Mouse Rat IgG2b M1/70 BioLegend 101208 PE AB_312791 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Prostate NA 0.3% Triton-X-100 NA NA Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P24788 Primary Antibody CD11b Mouse Rat IgG2b 5C6 Bio-Rad MCA711F FITC AB_1100617 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P24788 Primary Antibody CD11b Mouse Rat IgG2b M1/70 BioLegend 101208 PE AB_312791 Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P24788 Primary Antibody CD11b Mouse Rat IgG2b 5C6 Bio-Rad MCA711F FITC AB_1100617 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P24788 Primary Antibody CD11b Mouse Rat IgG2b 5C6 Bio-Rad MCA711F FITC AB_1100617 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P24788 Primary Antibody CD11b Mouse Rat IgG2b 5C6 Bio-Rad MCA711F FITC AB_1100617 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11215 Primary Antibody CD11b Human Rabbit IgG Polyclonal Novus Biologicals NB110-89474AF488 AF488 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11215 Primary Antibody CD11b Human Rabbit IgG EPR1344 Abcam ab133357 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11215 Primary Antibody CD11b Human Rabbit IgG EP1345Y Abcam ab187537 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11215 Primary Antibody CD11b Human Rabbit IgG SP330 Abcam ab241408 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11215 Primary Antibody CD11b Human Rabbit IgG SP331 Abcam ab238794 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P24788 Primary Antibody CD11b Mouse Rat IgG2b 5C6 Bio-Rad MCA711PE PE AB_323678 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11215 Primary Antibody CD11b Human Rabbit IgG EP1345Y Abcam ab187537 (Unconjugated) AF647 NA Custom IBEX2D Manual FFPE Tonsil NA 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P11215 Primary Antibody CD11b Human Rabbit IgG EP1345Y Abcam ab187537 (Unconjugated) AF647 NA Custom IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117371 Spark Red 718 AB_2924457 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117346 AF594 AB_2563323 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 Thermo Fisher Scientific MCD11c20 AF488 AB_10373244 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20702 Primary Antibody CD11c Human Mouse BALB/c IgG1 B-Ly6 BD Biosciences 561352 AF700 AB_10612006 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117308 PE AB_313777 Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967, 0000-0003-1118-7432 NA 0000-0003-4379-8967
P20702 Primary Antibody CD11c Human Mouse BALB/c IgG1 B-Ly6 BD Biosciences 562561 BV421 AB_2737656 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20702 Primary Antibody CD11c Human Mouse BALB/c IgG1 B-Ly6 BD Biosciences 563026 BV510 AB_2737960 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20702 Primary Antibody CD11c Human Mouse BALB/c IgG1 B-Ly6 BD Biosciences 561352 AF700 AB_10612006 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20702 Primary Antibody CD11c Human Rabbit IgG EP1347Y Abcam ab216655 (Unconjugated) AF555 NA Custom IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P20702 Primary Antibody CD11c Human Mouse BALB/c IgG1 B-Ly6 BD Biosciences 561355 FITC AB_10611872 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20702 Primary Antibody CD11c Human Mouse BALB/c IgG1 B-Ly6 BD Biosciences 561352 AF700 AB_10612006 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P20702 Primary Antibody CD11c Human Mouse BALB/c IgG1 B-Ly6 BD Biosciences 555392 PE AB_395793 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20702 Primary Antibody CD11c Human Rabbit IgG EP1347Y Abcam ab216655 (Unconjugated) AF555 NA Custom IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P20702 Primary Antibody CD11c Human Mouse IgG1 3.9 BioLegend 301603 FITC AB_314173 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20702 Primary Antibody CD11c Human Rabbit IgG EP1347Y Abcam ab216655 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117312 AF647 AB_389328 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9QXH4 Primary Antibody CD11c Mouse Armenian Hamster IgG N418 BioLegend 117314 AF647 AB_492850 Stock Multiplexed 2D Imaging 4% PFA Fixed Frozen Brain Tumor 0.3% Triton-X-100 NA NA Yes 0000-0002-6944-6997 NA 0000-0002-6944-6997
P18827 Primary Antibody CD138 Human Mouse IgG1 MI15 BioLegend 356504 PE AB_2561878 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18827 Primary Antibody CD138 Human Mouse IgG1 MI15 BioLegend 356504 PE AB_2561878 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18828 Primary Antibody CD138 Mouse Rat IgG2a 281-2 BioLegend 142526 AF647 AB_2566239 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18827 Primary Antibody CD138 Human Mouse IgG1 MI15 BioLegend 356504 PE AB_2561878 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18827 Primary Antibody CD138 Human Mouse IgG1 MI15 BioLegend 356504 PE AB_2561878 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18827 Primary Antibody CD138 Human Goat IgG Polyclonal R&D Systems AF2780 Unconjugated AB_442186 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18827 Primary Antibody CD138 Human Mouse IgG1 MI15 BioLegend 356517 BV510 AB_2562661 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18827 Primary Antibody CD138 Human Mouse IgG1 MI15 AAT Bioquest 113800C0 iF594 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18827 Primary Antibody CD138 Human Mouse IgG1 MI15 BioLegend 356504 PE AB_2561878 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18828 Primary Antibody CD138 Mouse Rat IgG2a 281-2 BD Biosciences 562610 BV421 AB_11153126 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18828 Primary Antibody CD138 Mouse Rat IgG2a 281-2 BD Biosciences 562610 BV421 AB_11153126 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18828 Primary Antibody CD138 Mouse Rat IgG2a 281-2 BD Biosciences 562610 BV421 AB_11153126 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18827 Primary Antibody CD138 Human Mouse IgG1 MI15 BioLegend 356524 AF647 AB_2564250 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P18827 Primary Antibody CD138 Human Mouse IgG1 MI15 BioLegend 356524 AF647 AB_2564250 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08571 Primary Antibody CD14 Human Rabbit IgG EPR3653 Abcam ab226121 (Unconjugated) AF555 NA Custom IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P08571 Primary Antibody CD14 Human Rabbit IgG EPR3653 Abcam ab226121 (Unconjugated) AF555 NA Custom IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P08571 Primary Antibody CD14 Human Mouse IgG1 61D3 Thermo Fisher Scientific 58-0149-41 AF532 AB_11218093 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P33151 Primary Antibody CD144 (VE-Cadherin) Human Mouse IgG2a BV9 BioLegend 348513 AF647 AB_2563669 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22083 Primary Antibody CD15 Human Mouse IgM MMA BD Biosciences 347420 Unconjugated AB_400298 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22083 Primary Antibody CD15 Human Mouse IgM MMA BD Biosciences 347420 Unconjugated AB_400298 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P22083 Primary Antibody CD15 Human Mouse IgM MMA BD Biosciences 347420 Unconjugated AB_400298 Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
P22083 Primary Antibody CD15 Human Mouse IgM MMA BD Biosciences 347420 Unconjugated AB_400298 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P22083 Primary Antibody CD15 Human Mouse IgM MMA BioLegend 394702 (Unconjugated) AF532 NA Custom Multiplexed 2D Imaging 1% PFA Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA Yes 0000-0002-2950-2683 NA 0000-0002-2950-2683
P22083 Primary Antibody CD15 Human Mouse IgM MMA BioLegend 394702 (Unconjugated) AF532 NA Custom Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22083 Primary Antibody CD15 Human Mouse IgM MMA BioLegend 394702 Unconjugated NA Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22083 Primary Antibody CD15 Human Mouse IgM MMA BD Biosciences 347420 Unconjugated AB_400298 Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
A0A0B4J1G0 Primary Antibody CD16.2 Mouse Armenian Hamster IgG 9.00E+09 BioLegend 149503 PE AB_2565810 Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P08101 Primary Antibody CD16/32 Mouse Goat IgG Polyclonal R&D Systems AF1460-SP Unconjugated NA Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA NA Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 GHI/61 BioLegend 333620 AF647 AB_2563475 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 GHI/61 Thermo Fisher Scientific 12-1639-41 PE AB_1963571 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 GHI/61 BioLegend 333620 AF647 AB_2563475 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 GHI/61 BioLegend 333628 BV510 AB_2650632 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q86VB7 Primary Antibody CD163 Human Mouse IgG2b EPR19518 Abcam ab213612 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 GHI/61 BioLegend 333602 (Unconjugated) AF532 AB_2892759 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 GHI/61 BioLegend 333602 (Unconjugated) AF532 AB_2892759 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 GHI/61 BioLegend 333611 BV421 AB_2562462 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 10D6 Thermo Fisher Scientific MA5-11458 Unconjugated AB_10982556 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
F7DAU3 Primary Antibody CD163 Rhesus Macaque Mouse IgG2b EDHu-1 Novus Biologicals NB110-40686AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Infected 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 GHI/61 BioLegend 333620 AF647 AB_2563475 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q86VB7 Primary Antibody CD163 Human Mouse IgG2b 6D3.2F3 Novus Biologicals NBP2-36495AF488 AF488 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 EDHu-1 Novus Biologicals NB110-40686AF647 AF647 NA Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 10D6 Thermo Fisher Scientific MA5-11458 Unconjugated AB_10982556 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
Q86VB7 Primary Antibody CD163 Human Mouse IgG1 10D6 Thermo Fisher Scientific MA5-11458 Unconjugated AB_10982556 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q13740 Primary Antibody CD166 Human Mouse IgG1 3A6 BioLegend 343903 PE NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Pancreas NA 0.3% Triton-X-100 NA NA Yes 0000-0002-3882-457X NA 0000-0002-3882-457X
Q13740 Primary Antibody CD166 Human Rabbit IgG EPR2759 Abcam ab197543 AF488 AB_2889210 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q62230 Primary Antibody CD169 Mouse Rat IgG2a 3D6.112 BioLegend 142404 PE AB_10915697 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967, 0000-0003-1118-7432 NA 0000-0003-4379-8967
Q62230 Primary Antibody CD169 Mouse Rat IgG2a 3D6.112 BioLegend 142404 PE AB_10915697 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q62230 Primary Antibody CD169 Mouse Rat IgG2a 3D6.112 BioLegend 142416 AF594 AB_2565620 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q62230 Primary Antibody CD169 Mouse Rat IgG2a 3D6.112 BioLegend 142404 PE AB_10915697 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q62230 Primary Antibody CD169 Mouse Rat IgG2a 3D6.112 BioLegend 142404 PE AB_10915697 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q62230 Primary Antibody CD169 Mouse Rat IgG2a 3D6.112 Bio-Rad MCA884F FITC AB_324246 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P49682 Primary Antibody CD183 (CXCR3) Human Rabbit IgG Polyclonal Novus Biologicals NBP2-16044 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P49682 Primary Antibody CD183 (CXCR3) Human Rabbit IgG Polyclonal Abcam ab154033 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P49682 Primary Antibody CD183 (CXCR3) Human Mouse IgG1 G025H7 BioLegend 353711 AF647 AB_10962946 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P32302 Primary Antibody CD185 (CXCR5) Human Rat IgG2b RF8B2 BD Biosciences 552032 Unconjugated AB_394324 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P32302 Primary Antibody CD185 (CXCR5) Human Rat IgG2b RF8B2 BD Biosciences 552032 Unconjugated AB_394324 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P32302 Primary Antibody CD185 (CXCR5) Human Rat IgG2b RF8B2 BD Biosciences 552032 Unconjugated AB_394324 Stock IBEX2D Manual FFPE Lymph Node Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P32302 Primary Antibody CD185 (CXCR5) Human Rat IgG2b RF8B2 BD Biosciences 558112 AF488 AB_397034 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P25918 Primary Antibody CD19 Mouse Rat IgG2a 6D5 BioLegend 115552 AF594 AB_2563459 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Prostate NA 0.3% Triton-X-100 NA NA Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P15391 Primary Antibody CD19 Human Mouse IgG1 SJ25C1 Thermo Fisher Scientific 58-0198-41 AF532 AB_11219076 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P29017 Primary Antibody CD1c Human Mouse IgG1 L161 AAT Bioquest 10013150 AF555 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P29017 Primary Antibody CD1c Human Mouse IgG1 L161 BioLegend 331522 AF488 AB_10720182 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P29017 Primary Antibody CD1c Human Mouse IgG1 L161 BioLegend 331510 AF647 AB_1186032 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P29017 Primary Antibody CD1c Human Mouse IgG1 L161 BioLegend 331529 AF700 AB_2563656 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P29017 Primary Antibody CD1c Human Mouse IgG1 L161 BioLegend 331525 BV421 AB_10933249 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P29017 Primary Antibody CD1c Human Mouse IgG1 L161 BioLegend 331506 PE AB_1088999 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P29017 Primary Antibody CD1c Human Mouse IgG1 L161 BioLegend 331506 PE AB_1088999 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11609 Primary Antibody CD1d Mouse Rat IgG2b 1B1 BD Biosciences 553846 PE AB_2073521 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
H9YXP1 Primary Antibody CD20 Rhesus Macaque Mouse IgG2a L26 Thermo Fisher Scientific 50-0202-82 eF660 AB_11150959 Stock Multiplexed 2D Imaging FFPE Lymph Node Infected 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 50-0202-82 eF660 AB_11150959 Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 42-0202-82 eF615 AB_10853517 Stock IBEX2D Manual FFPE Lymph Node Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11836 Primary Antibody CD20 Human Mouse IgG2b 2H7 BioLegend 302322 AF700 AB_493753 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
H9YXP1 Primary Antibody CD20 Rhesus Macaque Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock Multiplexed 2D Imaging FFPE Lymph Node Infected 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
H9YXP1 Primary Antibody CD20 Rhesus Macaque Mouse IgG2b 2H7 BioLegend 302354 AF594 AB_2565619 Stock Multiplexed 2D Imaging FFPE Lymph Node Infected 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P19437 Primary Antibody CD20 Mouse Rat IgG2b SA275A11 BioLegend 150432 AF488 AB_2890699 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes No 0000-0002-1461-0999 NA 0000-0002-1461-0999
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 42-0202-82 eF615 AB_10853517 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 42-0202-82 eF615 AB_10853517 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
H9YXP1 Primary Antibody CD20 Rhesus Macaque Mouse IgG2a L26 Thermo Fisher Scientific 42-0202-82 eF615 AB_10853517 Stock Multiplexed 2D Imaging FFPE Lymph Node Infected 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Novus Biologicals NBP2-80486AF532 AF532 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 50-0202-82 eF660 AB_11150959 Stock IBEX2D Manual FFPE Lymph Node Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 50-0202-82 eF660 AB_11150959 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Novus Biologicals NBP2-80486AF750 AF750 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9ES57 Primary Antibody CD200 Mouse Rat IgG2a OX-90 BD Biosciences 565547 BV421 AB_2739289 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22897 Primary Antibody CD206 Human Mouse IgG2b 685645 R&D Systems MAB25341 Unconjugated AB_10890782 Stock Multiplexed 2D Imaging FFPE Lung NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22897 Primary Antibody CD206 Human Mouse IgG1 15-2 BioLegend 321130 PE/Dazzle AF594 AB_2616867 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Prostate NA 0.3% Triton-X-100 NA NA Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
Q61830 Primary Antibody CD206 Mouse Rat IgG2a C068C2 BioLegend 141717 BV421 AB_2562232 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q61830 Primary Antibody CD206 Mouse Rat IgG2a C068C2 BioLegend 141717 BV421 AB_2562232 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q61830 Primary Antibody CD206 Mouse Rat IgG2a C068C2 BioLegend 141717 BV421 AB_2562232 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q8VBX4 Primary Antibody CD207 Mouse Rat IgG2a eBioL31 Thermo Fisher Scientific 12-2075-80 PE AB_763453 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Mouse IgG1 Bu32 BioLegend 354902 (Unconjugated) AF532 AB_2892739 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Mouse IgG1 Bu32 BioLegend 354902 (Unconjugated) AF532 AB_2892739 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Mouse IgG1 Bu32 BioLegend 354902 (Unconjugated) AF488 NA Custom IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody CD21 Mouse Rat IgG2a 7.E.9 BioLegend 123413 Pacific Blue AB_2085159 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Rabbit IgG SP186 Abcam ab306325 PE NA Stock Cell DIVE-IBEX FFPE Lymph Node NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Mouse IgG1 Bu32 BioLegend 354909 FITC AB_2561574 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Mouse IgG1 Bu32 BioLegend 354902 (Unconjugated) AF532 AB_2892739 Custom IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Mouse IgG1 CR2/1952 Novus Biologicals NBP2-60733AF532 AF532 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Rabbit IgG SP186 Abcam ab240987 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Rabbit IgG SP186 Abcam ab306325 PE NA Stock Cell DIVE-IBEX FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond 1 mg/ml LiBH4 15 minutes No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Mouse IgG1 1F8 Novus Biologicals NBP1-22527 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Mouse IgG1 Bu32 BioLegend 354903 PE AB_2561406 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Rabbit IgG SP186 Abcam ab306325 PE NA Stock Cell DIVE-IBEX FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20023 Primary Antibody CD21 Human Rabbit IgG SP186 Abcam ab240987 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06734 Primary Antibody CD23 Human Mouse IgG1 EBVCS-5 BioLegend 338502 (Unconjugated) AF532 AB_2892740 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06734 Primary Antibody CD23 Human Mouse IgG1 EBVCS-5 BioLegend 338505 FITC AB_1501125 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P20693 Primary Antibody CD23 Mouse Rat IgG2a B3B4 BioLegend 101611 AF647 AB_493479 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06734 Primary Antibody CD23 Human Mouse IgG1 EBVCS-5 BioLegend 338502 (Unconjugated) AF532 AB_2892740 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06734 Primary Antibody CD23 Human Mouse IgG1 EBVCS-5 BioLegend 338502 (Unconjugated) AF532 AB_2892740 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06734 Primary Antibody CD23 Human Mouse IgG1 EBVCS-5 BioLegend 338521 BV421 AB_2562460 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01590 Primary Antibody CD25 Mouse Rat IgG1 PC61.5 Thermo Fisher Scientific 53-0251-82 AF488 AB_763472 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01589 Primary Antibody CD25 Human Mouse IgG2b CD25-4E3 Thermo Fisher Scientific 17-0257-42 APC AB_11218671 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01589 Primary Antibody CD25 Human Mouse IgG1 M-A251 BioLegend 356128 AF647 AB_2563588 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01589 Primary Antibody CD25 Human Mouse IgG1 M-A251 BioLegend 356128 AF647 AB_2563588 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08138 Primary Antibody CD271 (NGFR5) Human Mouse IgG1 NGFR5 Thermo Fisher Scientific MA5-13311 Biotin AB_10982271 Stock Multiplexed 2D Imaging FFPE Spleen NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08138 Primary Antibody CD271 (NGFR5) Human Mouse IgG1 NGRF5 BioLegend 818201 Unconjugated AB_2564818 Stock Multiplexed 2D Imaging FFPE Spleen NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9EP73 Primary Antibody CD274 (PD-L1) Mouse Rat IgG2b MIH1 BD Biosciences 753201 RY586 NA Stock Ce3D 4% PFA Fixed Agarose Lymph Node NA 1:10 BD PermWash NA NA Yes 0000-0002-0835-911X NA 0000-0002-0835-911X
P09055 Primary Antibody CD29 Mouse Armenian Hamster IgG HMB1-1 BioLegend 102214 AF647 AB_492831 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-1461-0999 NA 0000-0002-1461-0999
P07766 Primary Antibody CD3 Human Rabbit IgG SP7 Abcam ab16669 Unconjugated AB_443425 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100281 Spark Red 718 AB_2924440 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100281 Spark Red 718 AB_2924440 Stock Ce3D 4% PFA Fixed Agarose Lymph Node NA 1:10 BD PermWash NA NA Yes 0000-0002-5187-810X NA 0000-0002-5187-810X
P07766 Primary Antibody CD3 Human Rabbit IgG SP7 Abcam ab16669 Unconjugated AB_443425 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 Thermo Fisher Scientific 58-0038-42 AF532 AB_11218675 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100205 PE AB_312662 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Rabbit IgG SP7 Abcam ab16669 Unconjugated AB_443425 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P07766 Primary Antibody CD3 Human Rabbit IgG EP4426 Abcam ab208514 AF555 AB_443425 Stock IBEX2D Manual FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300416 AF647 AB_389332 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100210 AF488 AB_389301 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300408 PE AB_314062 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100210 AF488 AB_389301 Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100234 BV510 AB_2562555 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100228 BV421 AB_2562553 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300415 AF488 AB_389310 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Prostate NA 0.3% Triton-X-100 NA NA Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P07766 Primary Antibody CD3 Human Rabbit IgG EPR4426 Abcam ab208514 AF555 NA Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
P07766 Primary Antibody CD3 Human Rabbit IgG EP4426 Abcam ab208514 AF555 AB_443425 Stock IBEX2D Manual FFPE Cervix Cervical Cancer 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7586-9996 NA 0000-0002-7586-9996
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300415 AF488 AB_389310 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Rabbit IgG EP4426 Abcam ab208514 AF555 AB_443425 Stock IBEX2D Manual FFPE Skin NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4184-3867 NA 0000-0003-4184-3867
P07766 Primary Antibody CD3 Human Rabbit IgG SP7 Abcam ab16669 Unconjugated AB_443425 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300448 BV510 AB_2563468 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100214 Pacific Blue AB_493645 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Prostate NA 0.3% Triton-X-100 NA NA Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300402 (Unconjugated) AF555 NA Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 Thermo Fisher Scientific 58-0032-80 AF532 AB_11220474 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100240 AF594 AB_2563427 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967, 0000-0003-1118-7432 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Rabbit IgG EP4426 Abcam ab208514 AF555 AB_443425 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 Caprico Biotechnologies 1053135 iF594 AB_2892737 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 Novus Biologicals NB100-2726IR DL755 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100281 Spark Red 718 AB_2924440 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA NA Yes 0000-0003-1130-1899 NA 0000-0003-1130-1899
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 Caprico Biotechnologies 1053135 iF594 AB_2892737 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
G7NCB9 Primary Antibody CD3 Rhesus Macaque Mouse IgG1 F7.2.38 Agilent M7254 Unconjugated AB_2631163 Stock Multiplexed 2D Imaging FFPE Lymph Node Infected 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P07766 Primary Antibody CD3 Human Rabbit IgG EP4426 Abcam ab208514 AF555 AB_443425 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 Caprico Biotechnologies 1053135 iF594 AB_2892737 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Rabbit IgG EP4426 Abcam ab208514 AF555 AB_443425 Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 Caprico Biotechnologies 1053135 iF594 AB_2892737 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 Caprico Biotechnologies 1053135 iF594 AB_2892737 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P04234 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300487 Spark Blue 574 AB_2904329 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P04234 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300401 (Unconjugated) AF555 NA Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300446 AF594 AB_2563236 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BD Biosciences 557869 AF647 AB_396912 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P04234 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300483 Spark Violet 538 AB_2890733 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Rabbit IgG EP4426 Abcam ab208514 AF555 AB_443425 Stock Cell DIVE-IBEX FFPE Lymph Node NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BioLegend 100240 AF594 AB_2563427 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 AAT Bioquest 10032060 iF514 NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P04234 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300435 BV570 AB_10898117 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300446 AF594 AB_2563236 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Rabbit IgG EP4426 Abcam ab208514 AF555 AB_443425 Stock Cell DIVE-IBEX FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P07766 Primary Antibody CD3 Human Rabbit IgG CAL57 Cell IDx HI06B-005 UT016 NA Stock IBEX2D Manual FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3 Mouse Rat IgG2b 17A2 BD Biosciences 557869 AF647 AB_396912 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303102 (Unconjugated) AF532 NA Custom Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303106 PE AB_314332 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BioLegend 102516 AF647 AB_2161029 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303133 AF700 AB_2566326 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303106 PE AB_314332 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303110 AF488 AB_493074 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303106 PE AB_314332 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303106 PE AB_314332 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 C31.3+C31.7+C31.10 Novus Biologicals NBP2-47785AF700 AF700 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
A0A5F7Z8X0 Primary Antibody CD31 Rhesus Macaque Rabbit IgG Polyclonal Abcam ab28364 Unconjugated AB_726362 Stock Multiplexed 2D Imaging FFPE Lymph Node Infected 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BD Biosciences 553373 PE AB_394819 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BD Biosciences 553373 PE AB_394819 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 C31.3+C31.7+C31.10 Novus Biologicals NBP2-47785AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303112 AF647 AB_493076 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BioLegend 102515 AF647 AB_2161030 Stock Multiplexed 2D Imaging 4% PFA Fixed Frozen Brain Tumor 0.3% Triton-X-100 NA NA Yes 0000-0002-6944-6997 NA 0000-0002-6944-6997
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BioLegend 102520 AF594 AB_2563319 Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BioLegend 102520 AF594 AB_2563319 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Prostate NA 0.3% Triton-X-100 NA NA Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BioLegend 102520 AF594 AB_2563319 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BD Biosciences 553373 PE AB_394819 Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BioLegend 102514 AF488 AB_2161031 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303133 AF700 AB_2566326 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q08481 Primary Antibody CD31 Mouse Rat IgG2a MEC13.3 BD Biosciences 553373 PE AB_394819 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 C31.3+C31.7+C31.10 Novus Biologicals NBP2-47785AF700 AF700 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16284 Primary Antibody CD31 Human Mouse IgG1 WM59 BioLegend 303126 AF594 AB_2563303 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P28906 Primary Antibody CD34 Human Mouse IgG1 QBEND/10 Thermo Fisher Scientific MA1-10205 PE AB_11152571 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P28906 Primary Antibody CD34 Human Mouse IgG1 QBEND/10 Novus Biologicals FAB7227T AF594 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P28906 Primary Antibody CD34 Human Mouse IgG1 QBEND/10 Novus Biologicals FAB7227T AF594 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P28906 Primary Antibody CD34 Human Mouse IgG1 QBEND/10 Thermo Fisher Scientific MA1-10205 PE AB_11152571 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P28906 Primary Antibody CD34 Human Mouse IgG1 4H11 AAT Bioquest 103400C0 iF594 AB_2892741 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P28906 Primary Antibody CD34 Human Mouse IgG1 4H11 AAT Bioquest 103400C0 iF594 AB_2892741 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P28906 Primary Antibody CD34 Human Mouse IgG1 4H11 AAT Bioquest 103400C0 iF594 AB_2892741 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P28906 Primary Antibody CD34 Human Mouse IgG1 4H11 AAT Bioquest 103400C0 iF594 AB_2892741 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P28906 Primary Antibody CD34 Human Mouse IgG1 QBEND/10 Thermo Fisher Scientific MA1-10205 PE AB_11152571 Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17927 Primary Antibody CD35 Human Mouse IgG1 E11 BioLegend 333404 FITC AB_2085022 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17927 Primary Antibody CD35 Human Mouse IgG1 E11 BioLegend 333406 PE AB_2292231 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17927 Primary Antibody CD35 Human Mouse IgG1 E11 BioLegend 333406 PE AB_2292231 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17927 Primary Antibody CD35 Human Mouse IgG1 E11 BD Biosciences 565329 AF647 AB_2739186 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody CD35 Mouse Rat IgG2a 8C12 BD Biosciences 740132 BV510 AB_2739889 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17927 Primary Antibody CD35 Human Mouse IgG1 E11 BioLegend 333406 PE AB_2292231 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody CD35 Mouse Rat IgG2a 8C12 BD Biosciences 740132 BV510 AB_2739889 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967, 0000-0003-1118-7432 NA 0000-0003-4379-8967
P28907 Primary Antibody CD38 Human Mouse IgG1 HIT2 BioLegend 303503 FITC AB_314355 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P28907 Primary Antibody CD38 Human Mouse IgG1 HIT2 BioLegend 303524 AF700 AB_2072781 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56528 Primary Antibody CD38 Mouse Rat IgG2a 90 BioLegend 102732 BV421 AB_2734153 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0002-1461-0999 NA 0000-0002-1461-0999
P55772 Primary Antibody CD39 Human Mouse IgG1 A1 BioLegend 328205 FITC AB_940423 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P55772 Primary Antibody CD39 Human Mouse IgG1 A1 BioLegend 328205 FITC AB_940423 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P55772 Primary Antibody CD39 Human Mouse IgG1 A1 BioLegend 328208 PE AB_940429 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P22646 Primary Antibody CD3e Human Mouse IgG1 UCHT1 Bio-Rad MCA463SBV670 StarBright Violet 670 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0002-0835-911X NA 0000-0002-0835-911X
P22646 Primary Antibody CD3e Human Mouse IgG1 UCHT1 Bio-Rad MCA463SBB700 StarBright Blue 700 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0002-0835-911X NA 0000-0002-0835-911X
P22646 Primary Antibody CD3e Human Mouse IgG1 UCHT1 Bio-Rad MCA463SBV710 StarBright Violet 710 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0002-0835-911X NA 0000-0002-0835-911X
P06332 Primary Antibody CD4 Mouse Human IgG1 REA604 Miltenyi Biotec 130-123-894 PE-Vio 770 AB_2811571 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes No 0000-0002-6980-8900 NA 0000-0002-6980-8900
P06332 Primary Antibody CD4 Mouse Rat IgG2a RM4-5 BioLegend 100533 AF647 AB_493372 Stock Multiplexed 2D Imaging 4% PFA Fixed Frozen Spleen Healthy 0.3% Triton-X-100 NA NA Yes 0000-0002-6944-6997 NA 0000-0002-6944-6997
P06332 Primary Antibody CD4 Mouse Rat IgG2a RM4-5 BioLegend 100533 AF647 AB_493372 Stock Multiplexed 2D Imaging 4% PFA Fixed Frozen Brain Tumor 0.3% Triton-X-100 NA NA Yes 0000-0002-6944-6997 NA 0000-0002-6944-6997
P06332 Primary Antibody CD4 Mouse Rat IgG2b GK1.5 BioLegend 100443 BV421 AB_2562557 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P01730 Primary Antibody CD4 Human Rabbit IgG EPR6855 Abcam ab196372 AF488 AB_2889191 Stock Cell DIVE-IBEX FFPE Lymph Node NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Goat IgG Polyclonal R&D Systems FAB8165G AF488 AB_2728839 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P01730 Primary Antibody CD4 Human Mouse IgG1 RPA-T4 BioLegend 300519 AF488 AB_389311 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06332 Primary Antibody CD4 Mouse Rat IgG2b GK1.5 BioLegend 100449 BV510 AB_2564587 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06332 Primary Antibody CD4 Mouse Rat IgG2a RM4-5 BD Biosciences 557667 AF488 AB_396779 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Mouse IgG1 RPA-T4 Novus Biologicals NBP2-27216AF750 AF750 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16003 Primary Antibody CD4 Rhesus Macaque Goat IgG Polyclonal R&D Systems FAB8165G AF488 AB_2728839 Stock Multiplexed 2D Imaging FFPE Lymph Node Infected 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P16003 Primary Antibody CD4 Rhesus Macaque Goat IgG Polyclonal R&D Systems FAB8165N AF700 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Infected 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P01730 Primary Antibody CD4 Human Goat IgG Polyclonal R&D Systems AF-379-SP Unconjugated NA Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06332 Primary Antibody CD4 Mouse Rat IgG2a GK1.5 BioLegend 100446 AF594 AB_2563182 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Goat IgG Polyclonal R&D Systems AF-379-SP Unconjugated NA Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
P06332 Primary Antibody CD4 Mouse Human IgG1 REA1211 Miltenyi Biotec 130-123-209 VioGreen AB_2811462 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-6980-8900 NA 0000-0002-6980-8900
P01730 Primary Antibody CD4 Human Mouse IgG1 RPA-T4 Thermo Fisher Scientific 58-0049-42 AF532 AB_2802361 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06332 Primary Antibody CD4 Mouse Rat IgG2a RM4-5 Thermo Fisher Scientific 58-0042-80 AF532 AB_11219484 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06332 Primary Antibody CD4 Mouse Rat IgG2a RM4-5 Thermo Fisher Scientific 58-0042-80 AF532 AB_11219484 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Mouse IgG1 RPA-T4 AAT Bioquest 10041090 iF555 NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06332 Primary Antibody CD4 Mouse Rat IgG2a RM4-5 Thermo Fisher Scientific 41-0042-82 eF570 AB_2573595 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06332 Primary Antibody CD4 Mouse Rat IgG2b GK1.5 BD Biosciences 553730 PE AB_396634 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Mouse IgG1 RPA-T4 BioLegend 300520 AF647 AB_389333 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Goat IgG Polyclonal R&D Systems FAB8165G AF488 AB_2728839 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P01730 Primary Antibody CD4 Human Rabbit IgG EPR6855 Cell IDx HI06B-005 UT019 NA Stock IBEX2D Manual FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Rabbit IgG EPR6855 Abcam ab196372 AF488 AB_2889191 Stock Cell DIVE-IBEX FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Mouse IgG1 RPA-T4 BioLegend 300526 AF700 AB_493743 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Metastatic 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Mouse IgG1 RPA-T4 Thermo Fisher Scientific 58-0049-42 AF532 AB_2802361 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06332 Primary Antibody CD4 Mouse Human IgG1 REA604 Miltenyi Biotec 130-119-132 APC-Vio 770 AB_2733118 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0002-6980-8900 NA 0000-0002-6980-8900
P06332 Primary Antibody CD4 Mouse Human IgG1 REA1211 Miltenyi Biotec 130-128-234 Vio Bright R720 AB_2905066 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-6980-8900 NA 0000-0002-6980-8900
P01730 Primary Antibody CD4 Human Rabbit IgG EPR6855 Abcam ab196372 AF488 AB_2889191 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Mouse IgG1 RPA-T4 BioLegend 300520 AF647 AB_389333 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06332 Primary Antibody CD4 Mouse Human IgG1 REA604 Miltenyi Biotec 130-118-316 PE-Vio 615 AB_2728077 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0002-6980-8900 NA 0000-0002-6980-8900
P01730 Primary Antibody CD4 Human Mouse IgG1 RPA-T4 BioLegend 300520 AF647 AB_389333 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01730 Primary Antibody CD4 Human Mouse IgG1 RPA-T4 BioLegend 300520 AF647 AB_389333 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06332 Primary Antibody CD4 Mouse Human IgG1 REA604 Miltenyi Biotec 130-118-568 VioBlue AB_2733943 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes No 0000-0002-6980-8900 NA 0000-0002-6980-8900
P06332 Primary Antibody CD4 Mouse Human IgG1 REA604 Miltenyi Biotec 130-118-794 PerCP-Vio 700 AB_2733032 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0002-6980-8900 NA 0000-0002-6980-8900
P01730 Primary Antibody CD4 Human Rabbit IgG EPR6855 Abcam ab196372 AF488 AB_2889191 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103018 AF647 AB_493681 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103054 AF594 AB_2563183 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103018 AF647 AB_493681 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P15379 Primary Antibody CD44 Mouse Rat IgG2b IM7 BioLegend 103002 (Unconjugated) AF532 AB_2892768 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103002 (Unconjugated) AF532 AB_2892768 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103002 (Unconjugated) AF532 AB_2892768 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103016 AF488 AB_493679 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103016 AF488 AB_493679 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 AAT Bioquest 104410C0 iF594 AB_2935685 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103016 AF488 AB_493679 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103007 PE AB_312958 Stock Multiplexed 2D Imaging FFPE Thymus NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P15379 Primary Antibody CD44 Mouse Rat IgG2b IM7 BioLegend 103018 AF647 AB_493681 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P15379 Primary Antibody CD44 Mouse Rat IgG2b IM7 BioLegend 103018 AF647 AB_493681 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P15379 Primary Antibody CD44 Mouse Rat IgG2b IM7 BioLegend 103026 AF700 AB_493713 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103074 Spark YG 570 AB_2892265 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08575 Primary Antibody CD45 Human Mouse IgG1 2B11+PD7/26 Novus Biologicals NBP2-34528PE PE NA Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
P08575 Primary Antibody CD45 Human Mouse IgG2a F10-89-4 Caprico Biotechnologies 1016136 iF594 AB_2892743 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08575 Primary Antibody CD45 Human Mouse IgG2a F10-89-4 Caprico Biotechnologies 1016136 iF594 AB_2892743 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08575 Primary Antibody CD45 Human Mouse IgG2a F10-89-4 Caprico Biotechnologies 1016136 iF594 AB_2892743 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 Novus Biologicals NB100-77417IR DL755 NA Stock IBEX2D Manual 4% PFA Fixed Frozen Lung Infected Akoya Antibody Diluent NA 1 mg/ml LiBH4 15 minutes Yes 0000-0001-9189-7172 NA 0000-0001-9189-7172
P08575 Primary Antibody CD45 Human Mouse IgG1 HI30 BioLegend 304060 AF594 AB_2629599 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0002-3882-457X NA 0000-0002-3882-457X
P08575 Primary Antibody CD45 Human Mouse IgG2a F10-89-4 Caprico Biotechnologies 1016136 iF594 AB_2892743 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103128 AF700 AB_493715 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Infected Akoya Antibody Diluent NA 1 mg/ml LiBH4 15 minutes Yes 0000-0001-9189-7172 NA 0000-0001-9189-7172
P08575 Primary Antibody CD45 Human Mouse IgG2a F10-89-4 Caprico Biotechnologies 1016184 PE/iF594 NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08575 Primary Antibody CD45 Human Mouse IgG1 2B11 + PD7/26 Novus Biologicals NBP2-34528AF700 AF700 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08575 Primary Antibody CD45 Human Mouse IgG1 HI30 BioLegend 304018 AF647 AB_389336 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103128 AF700 AB_493715 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Uterus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-2634-6838 NA 0000-0003-2634-6838
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103124 AF647 AB_493533 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08575 Primary Antibody CD45 Human Mouse IgG1 2B11+PD7/26 Novus Biologicals NBP2-34528AF700 AF700 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103134 BV421 AB_2562559 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103138 BV510 AB_2563061 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103122 AF488 AB_493531 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08575 Primary Antibody CD45 Human Mouse IgG2a F10-89-4 Caprico Biotechnologies 1016184 PE/iF594 NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P08575 Primary Antibody CD45 Human Mouse IgG1 HI30 Thermo Fisher Scientific 58-0459-41 AF532 AB_11218084 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Metastatic 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 Thermo Fisher Scientific 58-0451-82 AF532 AB_11218871 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103128 AF700 AB_493715 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103134 BV421 AB_2562559 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103122 AF488 AB_493531 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P06800 Primary Antibody CD45 Mouse Rat IgG2b 30-F11 BioLegend 103128 AF700 AB_493715 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody CD45RA Human Mouse IgG1 5H9 BD Biosciences 556626 FITC AB_396498 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56199 Primary Antibody CD49a Human Mouse IgG1 TS2/7 BioLegend 328310 AF647 AB_2129242 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56199 Primary Antibody CD49a Human Mouse IgG1 TS2/7 BioLegend 328310 AF647 AB_2129242 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56199 Primary Antibody CD49a Human Mouse IgG1 TS2/7 BioLegend 328308 FITC AB_2129084 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56199 Primary Antibody CD49a Human Mouse IgG1 TS2/7 BioLegend 328310 AF647 AB_2129242 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56199 Primary Antibody CD49a Human Mouse IgG1 TS2/7 BioLegend 328310 AF647 AB_2129242 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56199 Primary Antibody CD49a Human Mouse IgG1 TS2/7 BioLegend 328310 AF647 AB_2129242 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
P56199 Primary Antibody CD49a Human Mouse IgG1 CL7207 Abcam ab243032 Unconjugated NA Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56199 Primary Antibody CD49a Human Mouse IgG1 TS2/7 BioLegend 328310 AF647 AB_2129242 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56199 Primary Antibody CD49a Human Mouse IgG1 TS2/7 BioLegend 328303 PE AB_1236441 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56199 Primary Antibody CD49a Human Mouse IgG1 CL7207 Abcam ab243032 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P56199 Primary Antibody CD49a Human Mouse IgG1 TS2/7 BioLegend 328303 PE AB_1236407 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-3882-457X NA 0000-0002-3882-457X
P05362 Primary Antibody CD54 Human Mouse IgG1 HA58 BioLegend 353114 AF647 AB_2715942 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P05362 Primary Antibody CD54 Human Mouse IgG1 HA58 BioLegend 353114 AF647 AB_2715942 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P05362 Primary Antibody CD54 Human Mouse IgG1 HA58 BioLegend 353114 AF647 AB_2715942 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P05362 Primary Antibody CD54 Human Mouse IgG1 HA58 BioLegend 353114 AF647 AB_2715942 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P05362 Primary Antibody CD54 Human Mouse IgG1 HA58 BioLegend 353114 AF647 AB_2715942 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P05362 Primary Antibody CD54 Human Mouse IgG1 HA58 BioLegend 353125 AF700 AB_2810560 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P13591 Primary Antibody CD56 Human Mouse IgG1 123C3.D5 Novus Biologicals NBP2-33132AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P13591 Primary Antibody CD56 Human Mouse IgG1 123C3.D5 Novus Biologicals NBP2-33132AF647 AF647 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P13591 Primary Antibody CD56 Human Mouse IgG1 123C3 Bio-Rad MCA2693A488T AF488 AB_1102209 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P13591 Primary Antibody CD56 Human Mouse IgG2a MEM-188 BioLegend 304603 FITC AB_314445 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P13591 Primary Antibody CD56 Human Mouse IgG2a MEM-188 BioLegend 304611 AF488 AB_493179 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P13591 Primary Antibody CD56 Human Mouse IgG2a MEM-188 BioLegend 304605 PE AB_314447 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Ileum NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
F7DRX3 Primary Antibody CD57 Human Mouse IgM HNK-1 BioLegend 359602 Unconjugated AB_2562403 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
F7DRX3 Primary Antibody CD57 Human Mouse IgM HNK-1 BioLegend 359602 Unconjugated AB_2562403 Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P05106 Primary Antibody CD61 Human Mouse IgG1 Y2/51 Miltenyi Biotec 130-098-683 FITC AB_2660493 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0002-3882-457X NA 0000-0002-3882-457X
P26151 Primary Antibody CD64 Mouse Mouse IgG1 X54-5/7.1 BioLegend 139322 AF647 AB_2566561 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P26151 Primary Antibody CD64 Mouse Mouse IgG1 X54-5/7.1 BioLegend 139304 PE AB_10612740 Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P13688 Primary Antibody CD66abce Rhesus Macaque Mouse IgG2b Tet2 Miltenyi Biotec 130-116-522 FITC AB_2727588 Stock Opal-plex 10% Formalin for 7 Days Lung Infected Akoya Antibody Diluent Akoya AR6 NA Yes 0000-0003-0666-4804 NA 0000-0003-0666-4804
P31997 Primary Antibody CD66b Human Mouse IgM G10F5 BioLegend 305110 AF647 AB_2563171 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P31997 Primary Antibody CD66b Human Mouse IgM G10F5 Caprico Biotechnologies 1075136 iF594 AB_2892744 Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P31996 Primary Antibody CD68 Mouse Rat IgG2a FA-11 BioLegend 137004 AF647 AB_2044002 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Uterus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-2634-6838 NA 0000-0003-2634-6838
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Santa Cruz Biotechnology sc-20060 AF647 AF647 AB_3073741 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Caprico Biotechnologies 1064135 iF594 AB_2892745 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P31996 Primary Antibody CD68 Mouse Rat IgG2a FA-11 BioLegend 137017 BV421 AB_2562949 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P31996 Primary Antibody CD68 Mouse Rat IgG2a FA-11 BioLegend 137017 BV421 AB_2562949 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Caprico Biotechnologies 1064135 iF594 AB_2892745 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P31996 Primary Antibody CD68 Mouse Rat IgG2a FA-11 BioLegend 137011 AF488 AB_2074847 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P31996 Primary Antibody CD68 Mouse Rat IgG2a FA-11 BioLegend 137017 BV421 AB_2562949 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P31996 Primary Antibody CD68 Mouse Rat IgG2a FA-11 BioLegend 137004 AF647 AB_2044002 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0001-5813-2581 NA 0000-0001-5813-2581
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Santa Cruz Biotechnology sc-20060 AF647 AF647 AB_3073741 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Caprico Biotechnologies 1064135 iF594 AB_2892745 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Caprico Biotechnologies 1064135 iF594 AB_2892745 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Caprico Biotechnologies 1064135 iF594 AB_2892745 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Caprico Biotechnologies 1064135 iF594 AB_2892745 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P31996 Primary Antibody CD68 Mouse Rat IgG2a FA-11 BioLegend 137017 BV421 AB_2562949 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P31996 Primary Antibody CD68 Mouse Rat IgG2a FA-11 BioLegend 137004 AF647 AB_2044002 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-1461-0999 NA 0000-0002-1461-0999
P31996 Primary Antibody CD68 Mouse Rat IgG2a FA-11 BioLegend 137004 AF647 AB_2044002 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-1461-0999 NA 0000-0002-1461-0999
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Caprico Biotechnologies 1064135 iF594 AB_2892745 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Santa Cruz Biotechnology sc-20060 AF647 AF647 AB_3073741 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Caprico Biotechnologies 1064135 iF594 AB_2892745 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Caprico Biotechnologies 1064135 iF594 AB_2892745 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Santa Cruz Biotechnology sc-20060 AF647 AF647 AB_3073741 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P34810 Primary Antibody CD68 Human Mouse IgG1 KP1 Caprico Biotechnologies 1064135 iF594 AB_2892745 Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
Q07108 Primary Antibody CD69 Human Mouse IgG1 FN50 BioLegend 310918 AF647 AB_528871 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q07108 Primary Antibody CD69 Human Mouse IgG1 FN50 BioLegend 310918 AF647 AB_528871 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q07108 Primary Antibody CD69 Human Mouse IgG1 FN50 BioLegend 310906 PE AB_314841 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA NA No 0000-0002-3218-4479 NA 0000-0002-3218-4479
Q07108 Primary Antibody CD69 Human Mouse IgG2a 15B5G2 Novus Biologicals NBP2-25236AF532 AF532 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q61503 Primary Antibody CD73 Mouse Rat IgG1 TY/11.8 BioLegend 127230 AF700 AB_2800625 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes No 0000-0003-2634-6838 NA 0000-0002-1461-0999
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372910 Spark YG 570 AB_2904405 Stock IBEX2D Manual FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01731 Primary Antibody CD8 Mouse Rat IgG2a 53-6.7 BioLegend 100758 AF594 AB_2563237 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock IBEX2D Manual FFPE Skin Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7586-9996 NA 0000-0002-7586-9996
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock IBEX2D Manual FFPE Heart NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7586-9996 NA 0000-0002-7586-9996
P01731 Primary Antibody CD8 Mouse Rat IgG2a 53-6.7 BioLegend 100724 AF647 AB_389326 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 RPA-T8 Thermo Fisher Scientific 58-0088-24 AF532 AB_11220473 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 RPA-T8 BD Biosciences 557945 AF700 AB_396953 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 SK1 AAT Bioquest 100810C0 iF594 NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock IBEX2D Manual FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4184-3867 NA 0000-0003-4184-3867
P01732 Primary Antibody CD8 Human Mouse IgG1 SK1 BioLegend 344716 AF488 AB_10549301 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B Thermo Fisher Scientific 14-0085-82 Unconjugated AB_11150240 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 SK1 BioLegend 344716 AF488 AB_10549301 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 SK1 BioLegend 344716 AF488 AB_10549301 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 NA Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
P01732 Primary Antibody CD8 Human Mouse IgG1 SK1 BioLegend 344716 AF488 AB_10549301 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 SK1 BioLegend 344716 AF488 AB_10549301 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 SK1 BioLegend 344716 AF488 AB_10549301 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01731 Primary Antibody CD8 Mouse Rat IgG2a 53-6.7 BioLegend 100752 BV510 AB_2563057 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01731 Primary Antibody CD8 Mouse Rat IgG2a 53-6.7 BioLegend 100723 AF488 AB_389304 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock IBEX2D Manual FFPE Cervix Cervical Cancer 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7586-9996 NA 0000-0002-7586-9996
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01731 Primary Antibody CD8 Mouse Rat IgG2a 53-6.7 BioLegend 100738 BV421 AB_11204079 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01731 Primary Antibody CD8 Mouse Rat IgG2a 53-6.7 BioLegend 100738 BV421 AB_11204079 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01731 Primary Antibody CD8 Mouse Rat IgG2a 53-6.7 BioLegend 100738 BV421 AB_11204079 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01731 Primary Antibody CD8 Mouse Rat IgG2a 53-6.7 BD Biosciences 553032 PE AB_394571 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01731 Primary Antibody CD8 Mouse Rat IgG2a 53-6.7 BioLegend 100738 BV421 AB_11204079 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P01732 Primary Antibody CD8 Human Rabbit IgG EPR10640 Cell IDx HI06B-005 UT015 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01731 Primary Antibody CD8 Mouse Rat IgG2a 53-6.7 BioLegend 100738 BV421 AB_11204079 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P33681 Primary Antibody CD80 Human Goat IgG Polyclonal R&D Systems AF140 Unconjugated AB_354778 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P33681 Primary Antibody CD80 Human Mouse IgG2b 62N3G8 Novus Biologicals NBP2-25255AF488 AF488 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
P33681 Primary Antibody CD80 Human Mouse IgG1 2D10 BioLegend 305215 AF647 AB_528874 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q01151 Primary Antibody CD83 Human Mouse IgG1 HB15e BioLegend 305314 AF488 AB_528877 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q01151 Primary Antibody CD83 Human Mouse IgG1 HB15e BD Biosciences 550634 PE AB_393794 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P42081 Primary Antibody CD86 Human Goat IgG Polyclonal R&D Systems AF-141-NA Unconjugated AB_354385 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P42081 Primary Antibody CD86 Human Mouse IgG2b IT2.2 BioLegend 305415 AF647 AB_528882 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P42082 Primary Antibody CD86 (B7-2) Mouse Rat IgG2a GL1 R&D Systems FAB741S-100UG AF750 NA Stock Ce3D 4% PFA Fixed Agarose Lymph Node NA 1:10 BD PermWash NA NA No 0000-0002-0835-911X NA 0000-0002-0835-911X
P01731 Primary Antibody CD8a Mouse Rat IgG2a 53-6.7 R&D Systems FAB116S-100UG AF750 NA Stock Ce3D 4% PFA Fixed Agarose Lymph Node NA 1:10 BD PermWash NA NA No 0000-0002-0835-911X NA 0000-0002-0835-911X
P01732 Primary Antibody CD8a Mouse Mouse IgG1 HIT8a BioLegend 300908 PE AB_314112 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Prostate NA 0.3% Triton-X-100 NA NA Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P01831 Primary Antibody CD90.1 Mouse Mouse IgG1 OX-7 BioLegend 202508 AF647 AB_492884 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-1461-0999 NA 0000-0002-1461-0999
Q13241 Primary Antibody CD94 Human Mouse IgG1 DX22 BioLegend 305506 PE AB_314536 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q13241 Primary Antibody CD94 Human Mouse IgG1 DX22 BioLegend 305506 AF488 AB_314536 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q13241 Primary Antibody CD94 Human Mouse IgG1 DX22 BioLegend 305504 FITC AB_314534 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q13241 Primary Antibody CD94 Human Mouse IgG1 DX22 BioLegend 305512 AF647 AB_2249691 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q13241 Primary Antibody CD94 Human Mouse IgG1 DX22 BioLegend 305506 PE AB_314536 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9NXV6 Primary Antibody CDKN2a Human Rat IgG2b 5-C3-1 Novus Biologicals NB200-174AF647 AF647 AB_10002046 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9NXV6 Primary Antibody CDKN2a Human Mouse IgG2a ARF 4C6/4 Santa Cruz Biotechnology sc-53392 Unconjugated AB_630056 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P13569 Primary Antibody CFTR Human Mouse IgM CF3 Thermo Fisher Scientific MA1-935 Unconjugated AB_2081230 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond NA Yes 0000-0002-2950-2683 NA 0000-0002-2950-2683
P13569 Primary Antibody CFTR Human Mouse IgG1 M3A7 Thermo Fisher Scientific MA5-11768 Unconjugated AB_10983660 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Pancreas NA 0.3% Triton-X-100 NA NA No 0000-0002-2950-2683 NA 0000-0002-2950-2683
P13569 Primary Antibody CFTR Human Mouse IgM CF3 Thermo Fisher Scientific MA1-935 Unconjugated AB_2081230 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Pancreas NA 0.3% Triton-X-100 NA NA No 0000-0002-2950-2683 NA 0000-0002-2950-2683
NA Secondary Antibody Chicken IgY (H&L) Human Goat IgG Polyclonal Thermo Fisher Scientific A-32932 AF555 AB_2762844 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0002-5187-810X NA 0000-0002-5187-810X
NA Secondary Antibody Chicken IgY (H&L) Human Donkey IgG Polyclonal Thermo Fisher Scientific SA1-72000 FITC AB_923386 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10645 Primary Antibody Chromogranin A Human Mouse IgG1 LK2H10 Novus Biologicals NBP2-34672AF532 AF532 AB_2892553 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Pancreas NA 0.3% Triton-X-100 NA NA Yes 0000-0002-3882-457X NA 0000-0002-3882-457X
P58304 Primary Antibody Chx10 Mouse Mouse IgG2a E-12 Santa Cruz Biotechnology sc-365519 AF546 AF546 AB_10842442 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA NA Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
P70194 Primary Antibody CLEC4F Mouse Goat IgG Polyclonal R&D Systems AF2784 Unconjugated NA Stock Opal-plex 10% Formalin for 7 Days Liver Infected Akoya Antibody Diluent Akoya AR6 NA Yes 0000-0003-0666-4804 NA 0000-0003-0666-4804
Q6UXN8 Primary Antibody CLEC9a Human Mouse IgG2a 8F9 BioLegend 353802 (Unconjugated) AF488 NA Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BWP8 Primary Antibody COLEC11 Human Rabbit IgG Polyclonal Proteintech 15269-1-AP Unconjugated AB_10732940 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
P02462 Primary Antibody Collagen IV Mouse Rabbit IgG Polyclonal Abcam ab19808 Unconjugated AB_445160 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967, 0000-0003-1118-7432 NA 0000-0003-4379-8967
P02462 Primary Antibody Collagen IV Human Rabbit IgG Polyclonal Abcam ab6586 NA AB_305584 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P02462 Primary Antibody Collagen IV Human Rabbit IgG Polyclonal Abcam ab6586 Unconjugated AB_305584 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P02462 Primary Antibody Collagen IV Mouse Rabbit IgG Polyclonal Abcam ab19808 Unconjugated AB_445160 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P02462 Primary Antibody Collagen IV Mouse Rabbit IgG Polyclonal Abcam ab19808 Unconjugated AB_445160 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P02462 Primary Antibody Collagen IV Mouse Rabbit IgG Polyclonal Abcam ab19808 Unconjugated AB_445160 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9EQP6 Primary Antibody Cone Arrestin Mouse Rabbit IgG Polyclonal MilliporeSigma AB15282 Unconjugated AB_1163387 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA NA Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
Q6FHL8 Primary Antibody CTGF Human Rabbit IgG OT11F4 FabGennix CTGF-FITC FITC NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16410 Primary Antibody CTLA4 Human Rabbit IgG CAL49 Abcam ab270127 AF647 NA Stock Multiplexed 2D Imaging FFPE Lymph node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16410 Primary Antibody CTLA4 Human Rabbit IgG CAL49 Abcam ab270127 AF647 NA Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q6ICW0 Primary Antibody CXCL12 Human Mouse IgG1 79018 R&D Systems MAB350-500 (Unconjugated) AF532 NA Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q6ICW0 Primary Antibody CXCL12 Human Mouse IgG1 79018 R&D Systems MAB350 Unconjugated AB_2088149 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P63158 Primary Antibody CXCL12 Mouse Rabbit IgG polyclonal Abcam AB25117 NA AB_2088164 Stock Multiplexed 2D Imaging 4% PFA Fixed Frozen Brain Tumor 0.3% Triton-X-100 NA NA Yes 0000-0002-6944-6997 NA 0000-0002-6944-6997
Q53X90 Primary Antibody CXCL13 Human Goat IgG Polyclonal R&D Systems AF801 (Unconjugated) AF532 AB_2892755 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q53X90 Primary Antibody CXCL13 Human Goat IgG Polyclonal R&D Systems AF801 (Unconjugated) AF532 AB_2892755 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q53X90 Primary Antibody CXCL13 Human Goat IgG Polyclonal R&D Systems AF801 Unconjugated AB_2892755 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
Q53X90 Primary Antibody CXCL13 Human Goat IgG Polyclonal R&D Systems AF801 Unconjugated AB_2892755 Stock IBEX2D Manual FFPE Lymph Node Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P70658 Primary Antibody CXCR4 Mouse Rabbit IgG polyclonal Novus Biologicals NB-100 74396 NA AB_1048484 Stock Multiplexed 2D Imaging 4% PFA Fixed Frozen Brain Tumor 0.3% Triton-X-100 NA NA Yes 0000-0002-6944-6997 NA 0000-0002-6944-6997
Q9EQ16 Primary Antibody CXCR6 Mouse Rat IgG2b FAB2145R Novus Biologicals 221002 AF647 NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P56485 Primary Antibody CXCR7 Mouse Rabbit IgG polyclonal Novus Biologicals NBP2-24779G DyLight 488 NA Stock Multiplexed 2D Imaging 4% PFA Fixed Frozen Brain Tumor 0.3% Triton-X-100 NA NA Yes 0000-0002-6944-6997 NA 0000-0002-6944-6997
NA Primary Antibody Cytokeratin Mouse Mouse IgG1 C-11 BioLegend 628604 AF647 AB_2563652 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 Thermo Fisher Scientific 50-9003-82 eF660 AB_2574301 Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0003-3697-5726 NA 0000-0003-3697-5726
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 Thermo Fisher Scientific 53-9003-82 AF488 AB_2574301 Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 Thermo Fisher Scientific 53-9003-82 AF488 AB_2574301 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 Thermo Fisher Scientific 53-9003-82 AF488 AB_2574301 Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 BioLegend 914204 (Unconjugated) AF532 NA Custom Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA Yes 0000-0002-3882-457X NA 0000-0002-3882-457X
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 Thermo Fisher Scientific 53-9003-82 AF488 AB_2574301 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 Novus Biologicals NBP2-33200AF750 AF750 AB_2868569 Stock IBEX2D Manual FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-5187-810X NA 0000-0002-5187-810X
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 Thermo Fisher Scientific 53-9003-82 AF488 AB_2574301 Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 Thermo Fisher Scientific 42-9003-82 eF615 AB_10804663 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 Thermo Fisher Scientific 50-9003-82 eF660 AB_2574301 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody Cytokeratin (Pan) Human Mouse IgG1 AE1/AE3 Thermo Fisher Scientific 41-9003-80 eF570 AB_11217482 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P13645 Primary Antibody Cytokeratin 10 Human Mouse IgG1 DE-K10 Novus Biologicals NBP2-54402AF488 AF488 AB_2892746 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0002-5187-810X NA 0000-0002-5187-810X
P02533 Primary Antibody Cytokeratin 14 Human Chicken IgY Poly9060 BioLegend 906004 (Unconjugated) AF647 NA Custom Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P02533 Primary Antibody Cytokeratin 14 Human Chicken IgY Poly9060 BioLegend 906004 Unconjugated AB_2616962 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA NA Yes 0000-0002-5187-810X NA 0000-0002-5187-810X
P02533 Primary Antibody Cytokeratin 14 Human Chicken IgY Poly9060 BioLegend 906004 Unconjugated AB_2616962 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P05784 Primary Antibody Cytokeratin 18 Mouse Rabbit IgG Polyclonal Thermo Fisher Scientific PA5-14263 Unconjugated AB_2133178 Stock Opal-plex 10% Formalin for 7 Days Liver Infected Akoya Antibody Diluent Akoya AR6 NA Yes 0000-0003-0666-4804 NA 0000-0003-0666-4804
P08729 Primary Antibody Cytokeratin 7 Human Rat IgG2a W16155A BioLegend 601606 AF488 AB_2728458 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-3218-4479 NA 0000-0002-3218-4479
Q9NNX6 Primary Antibody DC-SIGN Human Mouse IgG2b 102E11.06 Eurobio Scientific (Dendritics) DDX0202A647-100 AF647 NA Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9NNX6 Primary Antibody DC-SIGN Human Rat IgG2a h209 LSBio LS-B3782 Unconjugated AB_10689801 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9NNX6 Primary Antibody DC-SIGN Human Rat IgG2a h209 LSBio LS-B3782 Unconjugated AB_10689801 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9NNX6 Primary Antibody DC-SIGN Human Mouse IgG2a DC28 R&D Systems MAB16211-SP Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9NNX6 Primary Antibody DC-SIGN Human Mouse IgG2b C209/1781 Novus Biologicals NBP2-54351AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9NNX6 Primary Antibody DC-SIGN Human Mouse IgG2a 9E9A8 BioLegend 330112 AF647 AB_1186092 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9NNX6 Primary Antibody DC-SIGN Human Mouse IgG2a 9E9A8 BioLegend 330112 AF647 AB_1186092 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9NNX6 Primary Antibody DC-SIGN Human Mouse IgG2b 102E11.06 Eurobio Scientific (Dendritics) DDX0202A647-100 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
O15075 Primary Antibody DCAMKL1 Human Rabbit IgG EPR6085 Abcam ab187184 Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
Q9JLM8 Primary Antibody DCAMKL1 Mouse Rabbit IgG Polyclonal Abcam ab37994 Unconjugated AB_873538 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
O15075 Primary Antibody DCAMKL1 Human Rabbit IgG Polyclonal Abcam ab31704 Unconjugated AB_873537 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q16832 Primary Antibody DDR2 Human Goat IgG Polyclonal R&D Systems AF2538 Unconjugated AB_416613 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q60767 Primary Antibody DEC205 Mouse Rat IgG2a NLDC-145 BioLegend 138204 AF647 AB_2137655 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q60767 Primary Antibody DEC205 Mouse Rat IgG2a NLDC-145 BioLegend 138204 AF647 AB_2137655 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17661 Primary Antibody Desmin Human Rabbit IgG Y66 Abcam ab185033 AF488 AB_2892748 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17661 Primary Antibody Desmin Human Rabbit IgG Y66 Abcam ab185033 AF488 AB_2892748 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17661 Primary Antibody Desmin Human Rabbit IgG Y66 Abcam ab185033 AF488 AB_2892748 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17661 Primary Antibody Desmin Human Rabbit IgG Y66 Abcam ab185033 AF488 AB_2892748 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
P17661 Primary Antibody Desmin Human Rabbit IgG Y66 Abcam ab185033 AF488 AB_2892748 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P17661 Primary Antibody Desmin Mouse Rabbit IgG Polyclonal Abcam ab15200 Unconjugated AB_301744 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA NA Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P09803 Primary Antibody E-cadherin Mouse Rat IgG1 DECMA-1 BioLegend 147308 AF647 AB_2563955 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P32249 Primary Antibody EBI2 Human Rabbit IgG Polyclonal RayBiotech 119-10961 Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P32249 Primary Antibody EBI2 Human Mouse IgG2a SA313E4 BioLegend 368903 AF647 AB_2566384 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P32249 Primary Antibody EBI2 Human Rabbit IgG Polyclonal Novus Biologicals NLS46 Unconjugated AB_10001402 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody EBOV NP Mouse Rabbit NA Polyclonal IBT BioServices 0301-012 Unconjugated NA Stock Opal-plex 10% Formalin for 7 Days Liver Infected Akoya Antibody Diluent Akoya AR6 NA Yes 0000-0003-0666-4804 NA 0000-0003-0666-4804
NA Primary Antibody EBOV NP Mouse Rabbit NA Polyclonal IBT BioServices 0301-012 Unconjugated NA Stock Opal-plex 10% Formalin for 7 Days Lymph Node Infected Akoya Antibody Diluent Akoya AR6 NA Yes 0000-0003-0666-4804 NA 0000-0003-0666-4804
Q99JW5 Primary Antibody EpCAM Mouse Rat IgG2a G8.8 BioLegend 118212 AF647 AB_1134101 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16422 Primary Antibody EpCAM Human Mouse IgG2b 9C4 BioLegend 324210 AF488 AB_756084 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16422 Primary Antibody EpCAM Human Rabbit IgG Polyclonal Abcam ab71916 Unconjugated AB_1603782 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16422 Primary Antibody EpCAM Human Mouse IgG2b 9C4 BioLegend 324228 AF594 AB_2563209 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Metastatic 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16422 Primary Antibody EpCAM Human Mouse IgG2b 9C4 BioLegend 324212 AF647 AB_756086 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q99JW5 Primary Antibody EpCAM Mouse Rat IgG2a G8.8 BioLegend 118222 AF594 AB_2563322 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16422 Primary Antibody EpCAM Human Mouse IgG2b 9C4 BioLegend 324202 (Unconjugated) AF532 NA Custom Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16422 Primary Antibody EpCAM Human Mouse IgG2b 9C4 BioLegend 324235 BV510 AB_2632934 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P16422 Primary Antibody EpCAM Human Mouse IgG2b 9C4 BioLegend 324243 AF700 AB_2750488 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
P16422 Primary Antibody EpCAM Human Mouse IgG1 1B7 Thermo Fisher Scientific 50-9326-42 eF660 AB_10598658 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q99JW5 Primary Antibody EpCAM Mouse Rat IgG2a G8.8 BD Biosciences 747748 BV510 AB_2872217 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q14246 Primary Antibody F4/80 Mouse Rat IgG2b F4/80 Abcam ab204467 AF647 AB_2810932 Stock IBEX2D Manual AntigenFix (2h, 4 degrees) Fixed Frozen Kidney NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
NA Primary Antibody F4/80 Mouse Rat IgG2a BM8 BioLegend 123132 BV421 AB_11203717 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody F4/80 Mouse Rat IgG2a BM8 BioLegend 123130 AF700 AB_2293450 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody F4/80 Mouse Rat IgG2a BM8 BioLegend 123132 BV421 AB_11203717 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q3S4B0 Primary Antibody F4/80 Mouse Rabbit IgG D2S9R Cell Signaling Technology 70076 Unconjugated NA Stock Opal-plex 10% Formalin for 7 Days Liver Infected Akoya Antibody Diluent Akoya AR6 NA Yes 0000-0003-0666-4804 NA 0000-0003-0666-4804
Q3S4B0 Primary Antibody F4/80 Mouse Rabbit IgG D2S9R Cell Signaling Technology 70076 Unconjugated NA Stock Opal-plex 10% Formalin for 7 Days Lymph Node Infected Akoya Antibody Diluent Akoya AR6 NA Yes 0000-0003-0666-4804 NA 0000-0003-0666-4804
Q14246 Primary Antibody F4/80 Mouse Rat IgG2b F4/80 Abcam ab204467 AF647 AB_2810932 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Prostate NA 0.3% Triton-X-100 NA NA Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
NA Primary Antibody F4/80 Mouse Rat IgG2a BM8 Thermo Fisher Scientific 12-4801-82 PE AB_465923 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody F4/80 Mouse Rat IgG2a BM8 BioLegend 123122 AF647 AB_893480 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Blocking Reagent Fc Block Human Human IgG1 Fc1 BD Biosciences 564220 Unconjugated AB_2869554 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Blocking Reagent Fc Block Mouse Rat IgG2b 2.4G2 BD Biosciences 553141 Unconjugated AB_394656 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q8TBJ5 Primary Antibody FEZF2 Human Rabbit IgG Polyclonal Abcam ab214186 Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
P02671 Primary Antibody Fibrinogen Human Sheep IgG Polyclonal Abcam ab118533 Unconjugated AB_10900171 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P02671 Primary Antibody Fibrinogen Human Sheep IgG Polyclonal Abcam ab118533 Unconjugated AB_10900171 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q12884 Primary Antibody Fibroblast activation protein alpha Human Rabbit IgG Polyclonal MilliporeSigma ABT11 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P02751 Primary Antibody Fibronectin Human Mouse IgG1 2F4 Novus Biologicals NBP2-22113AF532 AF532 NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Fluorescein/Oregon Green Human Rabbit IgG Polyclonal Thermo Fisher Scientific A-11090 AF488 AB_221562 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q12951 Primary Antibody FOXI1 Human Mouse IgG1 OTI1D4 Novus Biologicals NBP2-70747AF488 AF488 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
Q9H334 Primary Antibody FOXP1 Human Mouse IgG2a JC12 Novus Biologicals NB100-65125 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 53-4777-41 AF488 AB_10852836 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS2 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 17-4777-42 APC AB_10804651 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0002-2950-2683 NA 0000-0002-2950-2683
Q99JB6 Primary Antibody FOXP3 Mouse Rat IgG2a FJK-16s Thermo Fisher Scientific 12-5773-82 PE AB_465936 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 14-4777-82 Unconjugated AB_467556 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 50-4777-42 eF660 AB_2574219 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 14-4777-82 Unconjugated AB_467556 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 13-4777-82 Biotin AB_466655 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q99JB6 Primary Antibody FOXP3 Mouse Rat IgG2a FJK-16s Thermo Fisher Scientific 53-5773-82 AF488 AB_763537 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS2 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 17-4777-42 APC AB_10804651 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q99JB6 Primary Antibody FOXP3 Mouse Rat IgG2a FJK-16s Thermo Fisher Scientific 50-5773-82 eF660 AB_11220082 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q99JB6 Primary Antibody FOXP3 Mouse Rat IgG2a FJK-16s Thermo Fisher Scientific 58-5773-82 AF532 AB_11218870 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 41-4777-82 eF570 AB_2573609 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 41-4777-82 eF570 AB_2573609 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q99JB6 Primary Antibody FOXP3 Mouse Rat IgG2a FJK-16s Thermo Fisher Scientific 41-5773-82 eF570 AB_11219073 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 41-4777-82 eF570 AB_2573609 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 41-4777-82 eF570 AB_2573609 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 41-4777-82 eF570 AB_2573609 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q9BZS1 Primary Antibody FOXP3 Human Rabbit IgG 1054C Cell IDx HI06B-005 UT014 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q99JB6 Primary Antibody FOXP3 Mouse Rat IgG2a FJK-16s Thermo Fisher Scientific 50-5773-82 eF660 AB_11220082 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody Francisella Tularensis, LPS Mouse Mouse IgG2a 1.B.288 US Biological Life Sciences F6070-02X Unconjugated NA Stock IBEX2D Manual 4% PFA Fixed Frozen Lung Infected Akoya Antibody Diluent NA NA Yes 0000-0001-9189-7172 NA 0000-0001-9189-7172
Q14392 Primary Antibody GARP Human Mouse IgG2b Plato-1 Enzo ALX-804-867-C100 Unconjugated AB_2051806 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q14392 Primary Antibody GARP Human Mouse IgG2b 7B11 BioLegend 352503 PE AB_10896792 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q14392 Primary Antibody GARP Human Mouse IgG2b 7B11 BioLegend 352502 Unconjugated AB_10897447 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P23771 Primary Antibody GATA-3 Human Mouse IgG1 L50-823 BD Biosciences 560077 AF488 AB_1645303 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P03995 Primary Antibody GFAP Mouse Mouse IgG1 GA5 Thermo Fisher Scientific 41-9892-82 eF570 AB_2573656 Stock Multiplexed 2D Imaging 4% PFA Fixed Frozen Brain Tumor 0.3% Triton-X-100 NA NA Yes 0000-0002-6944-6997 NA 0000-0002-6944-6997
P14136 Primary Antibody GFAP Mouse Mouse IgG2b SMI 25 BioLegend 837504 AF647 AB_2632641 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
Q76IQ4 Primary Antibody Ghrelin Human Mouse IgG2b 891240 R&D Systems MAB8149 Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody GL-7 Mouse Rat IgM GL7 BD Biosciences 561530 PE AB_10715834 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Primary Antibody GL7 Mouse Rat IgM GL7 BD Biosciences 561530 PE AB_10715834 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P15104 Primary Antibody Glutamine synthetase Human Mouse IgG2a OTI1F4 Novus Biologicals NBP2-70834AF488 AF488 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P15104 Primary Antibody Glutamine synthetase Human Rabbit IgG Polyclonal Abcam ab49873 Unconjugated AB_880241 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA NA Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P15104 Primary Antibody Glutamine synthetase Human Rabbit IgG EPR13022(B) Abcam Ab302584 AF488 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P15104 Primary Antibody Glutamine synthetase Human Mouse IgG2a OTI1F4 Novus Biologicals NBP2-70834AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P15104 Primary Antibody Glutamine synthetase Mouse Rabbit IgG Polyclonal Abcam ab49873 Unconjugated AB_880241 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA NA Yes 0000-0003-1431-1398 NA 0000-0003-1431-1398
P15104 Primary Antibody Glutamine synthetase Human Rabbit IgG EPR13022(B) Abcam ab302584 AF488 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P15104 Primary Antibody Glutamine synthetase Human Mouse IgG2bc GLUL/6600 Novus Biologicals NBP3-14023AF488 AF488 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P15104 Primary Antibody Glutamine synthetase Mouse Mouse IgG1 1B6G4 Proteintech CL488-66323 CoraLite Plus AF488 AB_2883309 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
P02724 Primary Antibody Glycophorin A Human Rabbit IgG EPR8200 Abcam ab218372 (Unconjugated) AF555 AB_2892758 Custom IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P02724 Primary Antibody Glycophorin A Human Rabbit IgG EPR8200 Abcam ab218372 (Unconjugated) AF555 AB_2892758 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-3882-457X NA 0000-0002-3882-457X
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-11056 AF546 AB_2534103 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-11058 AF594 AB_2534105 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-32930 AF800 (Plus) AB_2762842 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-11055 AF488 AB_2534102 Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A32816 AF555 (Plus) AB_2762839 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-2088-8310 NA 0000-0003-2088-8310
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-21447 AF647 AB_2535864 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-21084 AF680 AB_2535741 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-11055 AF488 AB_2534102 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-21084 AF680 AB_2535741 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-21432 AF555 AB_2535853 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific SA5-10091 DL755 AB_2556671 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-21432 AF555 AB_2535853 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-21432 AF555 AB_2535853 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-21084 AF680 AB_2535741 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-3882-457X NA 0000-0002-3882-457X
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-11055 AF488 AB_2534102 Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-21432 AF555 AB_2535853 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Abcam ab175745 AF750 AB_2924800 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes No 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Secondary Antibody Goat IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific SA5-10091 DL755 AB_2556671 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q62011 Primary Antibody gp38 Mouse Syrian Hamster IgG 8.1.1 BioLegend 127401 Unconjugated AB_1089186 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10144 Primary Antibody Granzyme B Human Rabbit IgG EP230 Cell Marque 262R-17 Unconjugated AB_2889344 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P10144 Primary Antibody Granzyme B Human Rabbit IgG Polyclonal Abcam ab4059 Unconjugated AB_304251 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
F7CLT4 Primary Antibody Granzyme B Rhesus Macaque Mouse IgG2a GrB-7 Agilent M7235 Unconjugated AB_2114697 Stock Multiplexed 2D Imaging FFPE Lymph Node Infected 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) NA Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
NA Secondary Antibody Hamster IgG (H+L) Mouse Goat IgG Polyclonal Thermo Fisher Scientific A-21451 AF647 AB_2535868 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q64525 Primary Antibody Histone H2B Mouse Rat IgG2a W17025A BioLegend 606301 Unconjugated AB_2728465 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0002-5187-810X NA 0000-0002-5187-810X
P02302 Primary Antibody Histone H3 Mouse Mouse IgG3 1B1-B2 BioLegend 819406 AF594 AB_2715803 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0002-5187-810X NA 0000-0002-5187-810X
P68431 Primary Antibody Histone H3 (citrulline R2 + R8 + R17) Human Rabbit IgG Polyclonal Abcam ab5103 Unconjugated AB_304752 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X
P01903 Primary Antibody HLA-DR Human Mouse IgG2a LN-3 Novus Biologicals NBP2-47670AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 Caprico Biotechnologies 1032136 iF594 AB_2892750 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 Caprico Biotechnologies 1032136 iF594 AB_2892750 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307620 AF488 AB_493175 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2b LN-3 Novus Biologicals NBP2-47670 PE NA Stock IBEX2D Manual FFPE Lung Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-2692-1225 NA 0000-0002-2692-1225
P01903 Primary Antibody HLA-DR Human Mouse IgG1 TAL 1B5 Abcam ab20181 AF647 AB_445401 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Prostate NA 0.3% Triton-X-100 NA NA Yes 0000-0002-7422-3237 NA 0000-0002-7422-3237
P01903 Primary Antibody HLA-DR Human Mouse IgG2b LN-3 Novus Biologicals NBP2-47670 PE NA Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307620 AF488 AB_493175 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2b LN-3 Novus Biologicals NBP2-47670AF750 AF750 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307620 AF488 AB_493175 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 Caprico Biotechnologies 1032136 iF594 AB_2892750 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a LN-3 Novus Biologicals NBP2-47670PE PE NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a LN-3 Novus Biologicals NBP2-47670AF532 AF532 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 Novus Biologicals NB100-77855AF750 AF750 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307625 AF700 AB_493770 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2b LN-3 Novus Biologicals NBP2-47670AF488 AF488 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG1 TAL 1B5 Abcam ab176408 (Unconjugated) iF594 NA Custom IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307622 AF647 AB_493177 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG1 TAL 1B5 Abcam ab176408 (Unconjugated) iF594 NA Custom IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307602 (Unconjugated) AF532 NA Custom Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307619 AF488 AB_493176 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2b LN-3 Novus Biologicals NBP2-47670AF488 AF488 NA Stock Multiplexed 2D Imaging FFPE Lymph node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307620 AF488 AB_493175 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01903 Primary Antibody HLA-DR Human Mouse IgG2a LN-3 Novus Biologicals NBP2-47670AF647 AF647 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
P01911 Primary Antibody HLA-DRB Human Mouse IgG2b SPM288 Novus Biologicals NBP2-45312-0.02mg Unconjugated NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967
P63158 Primary Antibody HMGB-1 Mouse Mouse IgG2 3.00E+08 BioLegend 651406 AF594 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0002-5187-810X NA 0000-0002-5187-810X
NA Nuclear Dye Hoechst 33342 Human NA NA NA Biotium 40046 NA NA Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Nuclear Dye Hoechst 33342 Human NA NA NA Biotium 40046 NA NA Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Nuclear Dye Hoechst 33342 Human NA NA NA Biotium 40046 NA NA Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Skin NA 0.3% Triton-X-100 NA NA Yes 0000-0002-5187-810X NA 0000-0002-5187-810X
NA Nuclear Dye Hoechst 33342 Human NA NA NA Biotium 40046 NA NA Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Nuclear Dye Hoechst 33342 Human NA NA NA Biotium 40046 NA NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA NA Yes 0000-0002-3882-457X NA 0000-0002-3882-457X
NA Nuclear Dye Hoechst 33342 Human NA NA NA Biotium 40046 NA NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
NA Nuclear Dye Hoechst 33342 Human NA NA NA Biotium 40046 NA NA Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967
Q14576 Primary Antibody HuC/HuD neuronal protein Human Mouse IgG2b 16A11 Thermo Fisher Scientific A21271 Unconjugated AB_221448 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Retina NA 0.1% Saponin NA