Click the links in the Agree and Disagree columns to view validation images, notes, and accompanying publications for a listed reagent. View reagents based on your specific needs by filtering on the relevant criteria (protein biomarker, tissue preservation, etc.).
Additional information on fluorescent probes tested by the IBEX Imaging Community can be found here. Details on reagent validation can be found here.
Notes:
- Loading the filterable reagents table takes some time but is worth the wait.
- You can readily download the underlying comma separated value file for the reagent resources (“Save Link As…”) or download the whole knowledge-base as a zip file and go to the
datadirectory that contains all csv files. - Detailed descriptions and definitions for each column in the table are available here.
| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Images |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q9BYF1 | Primary Antibody | ACE2 | Human | Goat | IgG | Polyclonal | R&D Systems | AF933 (Unconjugated) | AF555 | AB_2892761 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9GZX7 | Primary Antibody | AID | Human | Rat | IgG2a | mAID-2 | Thermo Fisher Scientific | 14-5959-82 | Unconjugated | AB_10669583 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9GZX7 | Primary Antibody | AID | Human | Mouse | IgG1 | 39-2500 | Thermo Fisher Scientific | ZA001 | Unconjugated | AB_2533403 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | Alpha smooth muscle actin | Rhesus Macaque | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 | NHP spleen: CD20 (magenta, catalog number 50-0202-82) and a-SMA (green, catalog number 53-9760-82) |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | Alpha smooth muscle actin | Canine | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine lymph node FFPE: a-SMA (green, catalog number 53-9760-82) |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| NA | Primary Antibody | Alpha smooth muscle actin | Canine | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine melanoma skin FFPE: a-SMA (magenta, catalog number 41-9760-82) and pan-cytokeratin (green, catalog number 53-9003-80) |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: aSMA (cyan, catalog number 41-9760-82) and CD138 (red, catalog number 356504) |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Alpha smooth muscle actin (cyan, catalog number 53-9760-82) and Hoechst (blue, catalog number 40046) |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 41-9760-82 | eF570 | AB_2573631 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | Mouse small intestine: alpha smooth muscle actin (grey, catalogue number 53-9760-82) |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 50-9760-82 | eF660 | AB_2574362 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Abcam | ab184675 | AF488 | AB_2832195 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | Novus Biologicals | NBP2-34522IR | DL755 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: Alpha smooth muscle actin (red, catalog number 53-9760-82) |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | Novus Biologicals | NBP2-34522IR | DL755 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4 | Novus Biologicals | IC1420S-025 | AF750 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| P25054 | Secondary Antibody | APC | Human | Mouse | IgG2b | 936809 | R&D Systems | FAB8927R-100UG | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P29972 | Primary Antibody | Aquaporin 1 | Human | Rabbit | IgG | EPR11588(B) | Abcam | ab225225 | AF647 | AB_2810992 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P41181 | Primary Antibody | Aquaporin 2 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-33472 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P41181 | Primary Antibody | Aquaporin 2 | Human | Goat | IgG | Polyclonal | Novus Biologicals | NBP1-70378 | Unconjugated | AB_11011225 | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P05089 | Primary Antibody | Arginase-1 | Mouse | Rabbit | IgG | D4E3M | Cell Signaling Technology | 93668 | Unconjugated | AB_2800207 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 | NA |
| P36575 | Primary Antibody | Arrestin C | Human | Mouse | IgG1 | 7G6 | MilliporeSigma | MABN2636-100UG | Unconjugated | AB_2935804 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Arrestin C (green, catalog number MABN2636-100UG) and Hoechst (blue, catalog number 40046) |
| P00966 | Primary Antibody | ASS1 | Human | Rabbit | IgG | EPR12398 | Abcam | ab170952 | Unconjugated | AB_2892766 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103248 | BV510 | AB_2650679 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 557669 | AF488 | AB_396781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 | Mouse lymph node: B220 (cyan, catalog number 557669) and CD3 (red, catalog number 100240) |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 553090 | PE | AB_394619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 58-0452-82 | AF532 | AB_11218492 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 41-0452-80 | eF570 | AB_2573598 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 42-0452-82 | eF615 | AB_10852702 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103232 | AF700 | AB_493717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BD Biosciences | 557683 | AF647 | AB_396793 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2b | RA3-6B2 | BD Biosciences | 571680 | RY775 | AB_3678612 | Stock | Ce3D | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | No | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: 2D slice at the edge of a ~250um-thick 3D image, Immunized mouse lymph node: 2D slice at the centre of a ~250um-thick 3D image |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103251 | BV421 | AB_2562905 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 41-0452-80 | eF570 | AB_2573598 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103289 | Spark Blue 574 | AB_2904275 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 48-0452-82 | eF450 | AB_1548761 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103294 | Spark Red 718 | AB_2924441 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103294 | Spark Red 718 | AB_2924441 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | No | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2b | RA3-6B2 | BD Biosciences | 571680 | RY775 | AB_3678612 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | BioLegend | 103226 | AF647 | AB_389330 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | Mouse lymph node: B220 (grey, catalog number 103226) |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2b | RA3-6B2 | BD Biosciences | 571680 | RY775 | AB_3678612 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) but with signal maximally increased in Imaris to display the completeness of signal loss with dye inactivation., Immunized mouse lymph node: dye-inactivated section imaged maximized microscope settings (laser power, gain) and optimal Imaris setting for visualization to evaluate potential residual non-inactivated signal which might interfere with subsequent imaging cycles, especially of dim markers. |
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 46-0452-82 | PerCP-eF710 | AB_10717389 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Placenta | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658707 | PE | AB_2563281 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658703 | AF488 | AB_2563151 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658707 | PE | AB_2563281 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P10415 | Primary Antibody | BCL2 | Human | Mouse | IgG1 | 100 | BioLegend | 658705 | AF647 | AB_2563279 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: BCL2 (cyan, catalog number 658705) and CD3 (purple, catalog number 300446) |
| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human FL LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) |
| P10415 | Primary Antibody | BCL2 | Human | Rabbit | IgG | SP66 | Abcam | ab236221 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human normal LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) |
| P41182 | Primary Antibody | BCL6 | Human | Mouse | IgG1 | GI191E | Thermo Fisher Scientific | 14-9887-82 | Unconjugated | AB_10669166 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P41182 | Primary Antibody | BCL6 | Human | Mouse | IgG1 | K112-91 | BD Biosciences | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: BCL6 (red, catalog number 561525) and BCL2 (cyan, catalog number 658705) |
| P41182 | Primary Antibody | BCL6 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-59786 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P41183 | Primary Antibody | BCL6 | Mouse | Mouse | IgG1 | K112-91 | BD Biosciences | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P41182 | Primary Antibody | BCL6 | Human | Mouse | IgG1 | K112-91 | BD Biosciences | 561525 | AF647 | AB_10898007 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9W6A8 | Primary Antibody | Blue opsin | Zebrafish | Rabbit | IgG | Polyclonal | Kerafast | EJH012 | Unconjugated | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P03999 | Primary Antibody | Blue opsin | Human | Rabbit | IgG | Polyclonal | MilliporeSigma | AB5407 | Unconjugated | AB_177457 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Opsin Blue (yellow, catalog number AB5407) and Hoechst (blue, catalog number 40046) |
| P51491 | Primary Antibody | Blue opsin | Mouse | Rabbit | IgG | Polyclonal | MilliporeSigma | AB5407 | Unconjugated | AB_177457 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | NA |
| P35226 | Primary Antibody | BMI-1 | Human | Mouse | IgG2b | LLBmi1-1 | Novus Biologicals | NBP1-96140AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01106 | Primary Antibody | c-Myc | Human | Mouse | IgG1 | 9E10 | R&D Systems | MAB3696-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE : c-Myc (cyan, catalog number MAB3696-SP) |
| P01106 | Primary Antibody | c-Myc | Human | Mouse | IgG1 | 9E10 | Novus Biologicals | NB600-302IR | DL755 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| E9QJF8 | Primary Antibody | Calbindin | Zebrafish | Rabbit | IgG | Polyclonal | Swant | CB38a | Unconjugated | AB_10000340 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P05937 | Primary Antibody | Calbindin | Human | Rabbit | IgG | Polyclonal | MilliporeSigma | ABN2192 | Unconjugated | AB_2935805 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Calbindin (magenta, catalog number ABN2192) and Hoechst (blue, catalog number 40046) |
| A0A1A8U8H3 | Primary Antibody | Calretinin | Turquoise Killifish | Rabbit | IgG | Polyclonal | Swant | 7697 | Unconjugated | AB_2721226 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q6PC44 | Primary Antibody | Calretinin | Zebrafish | Rabbit | IgG | Polyclonal | Swant | 7697 | Unconjugated | AB_2721226 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P22676 | Primary Antibody | Calretinin | Mouse | Mouse | IgG1 | 6B8.2 | MilliporeSigma | MAB1568 | Unconjugated | AB_94259 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | NA |
| NA | Primary Antibody | Carbonic anhydrase 1 | Zebrafish | Rabbit | IgG | EPR5193 | Abcam | ab108367 | Unconjugated | AB_10863424 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P07711 | Primary Antibody | Cathepsin L | Human | Rat | IgG2a | 204101 | R&D Systems | MAB-9521 | Unconjugated | AB_2087829 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q99731 | Primary Antibody | CCL19 | Human | Mouse | IgG1 | OTI2A12 | Novus Biologicals | NBP2-71278AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| O15444 | Primary Antibody | CCL25 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-49199 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P32248 | Primary Antibody | CCR7 | Human | Goat | IgG | Polyclonal | Novus Biologicals | NB100-712SS | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P32248 | Primary Antibody | CCR7 | Human | Rabbit | IgG | Y59 | Abcam | ab32527 | Unconjugated | AB_726208 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P32248 | Primary Antibody | CCR7 | Human | Rabbit | IgG | Polyclonal | LSBio | LS-C210538 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P51686 | Primary Antibody | CCR9 | Human | Mouse | IgG2a | 112509 | Novus Biologicals | FAB179G-100UG | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P51686 | Primary Antibody | CCR9 | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-33407 | Unconjugated | AB_2550787 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | BioLegend | 312204 | PE | AB_314915 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 1039134 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | BioLegend | 312204 | PE | AB_314915 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 103924 | PE | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08473 | Primary Antibody | CD10 | Human | Rat | IgG2b | 200103 | R&D Systems | MAB1126-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | HI10a | BioLegend | 312204 | PE | AB_314915 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD10 (red, catalog number 312204) and Hoechst (cyan, catalog number 40046) |
| P08473 | Primary Antibody | CD10 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1182 | Unconjugated | AB_354652 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 103924 | PE | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08473 | Primary Antibody | CD10 | Human | Mouse | IgG1 | FR4D11 | Caprico Biotechnologies | 103901 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P38570 | Primary Antibody | CD103 | Human | Rabbit | IgG | ITGAE/3904R | Novus Biologicals | NBP3-08855AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P38570 | Primary Antibody | CD103 | Human | Rabbit | IgG | ITGAE/3904R | Novus Biologicals | NBP3-07530-100ug | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P17813 | Primary Antibody | CD105 (Endoglin) | Human | Mouse | IgG1 | SN6h | BioLegend | 800505 | FITC | AB_2687058 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P29533 | Primary Antibody | CD106 | Mouse | Rat | IgG2a | 429 | BioLegend | 105710 | AF488 | AB_493427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD206 (red, catalog number 141717) and CD106 (cyan, catalog number 105710) |
| P19320 | Primary Antibody | CD106 | Human | Mouse | IgG1 | STA | BioLegend | 305806 | PE | AB_314561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD23 (yellow, based on catalog number 338502) and CD106 (blue, catalog number 305806) |
| P19320 | Primary Antibody | CD106 | Human | Mouse | IgG1 | STA | BioLegend | 305806 | PE | AB_314561 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: Hoechst (blue, catalog number 40046), CD106 (cyan, catalog number 305806), and CD31 (red, catalog number 303106) |
| P29533 | Primary Antibody | CD106 | Mouse | Rat | IgG2a | 429 | Thermo Fisher Scientific | 50-1061-82 | eF660 | AB_11219864 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P10721 | Primary Antibody | CD117 | Human | Mouse | IgG1 | 104D2 | BioLegend | 313203 | PE | AB_314983 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | Human skin: CD117 (magenta, catalog number 313203), CD138 (cyan, catalog number 356524), and Vimentin (blue, catalog number 677804) |
| P10721 | Primary Antibody | CD117 | Human | Mouse | IgG1 | 104D2 | BioLegend | 313234 | AF488 | AB_2566221 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD35 (blue, catalog number 333406), CD117 (yellow, catalog number 313234), and CD66b (magenta, catalog number 305110), Human lymph node: CD117 (cyan, catalog number 313234), CD39 (blue, catalog number 328208), and CD94 (magenta, catalog number 305506) |
| O97799 | Primary Antibody | CD117 | Canine | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | BS-0672R | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | NA |
| P05532 | Primary Antibody | CD117 | Mouse | Rat | IgG2b | 2B8 | BioLegend | 105827 | BV421 | AB_10898120 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse gut: EpCAM (cyan, catalog number 118222), B220 (purple, catalog number 103248), and CD117 (yellow, catalog number 105827) |
| Q9UQ88 | Primary Antibody | CD11a | Human | Mouse | IgG1 | HI111 | AAT Bioquest | 101100C0 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB110-89474AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EPR1344 | Abcam | ab133357 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101208 | PE | AB_312791 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | Abcam | ab187537 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | Abcam | ab187537 (Unconjugated) | AF647 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | SP330 | Abcam | ab241408 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | SP331 | Abcam | ab238794 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | CD11b | Canine | Mouse | IgG1 | CA16.3E10 | Thermo Fisher Scientific | MA5-16604 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine lymph node FFPE: CD11b (red, catalog number MA5-16604 and A21203) |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711F | FITC | AB_1100617 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | 5C6 | Bio-Rad | MCA711PE | PE | AB_323678 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BD Biosciences | 101217 | PE | AB_396680 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-8909-5039 | NA | 0000-0002-8909-5039 | Mouse lymph node: CD11b (grey, catalog number 101217) |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: CD11c (cyan, catalog number 117312) and CD11b (yellow, catalog number 101217) |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P11215 | Primary Antibody | CD11b | Human | Rabbit | IgG | EP1345Y | Abcam | ab187537 (Unconjugated) | AF647 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101254 | AF594 | AB_2563231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: CD11b (yellow, catalog number 101254) and Vimentin (cyan, catalog number 699305) |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BD Biosciences | 557397 | PE | AB_396680 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | Mouse liver: CD4 (yellow, catalog number 100443), CD11c (cyan, catalog number 117312), and CD11b (purple, catalog number 101217) |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101217 | AF488 | AB_389305 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P24788 | Primary Antibody | CD11b | Mouse | Rat | IgG2b | M1/70 | BioLegend | 101208 | PE | AB_312791 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD54 (blue, catalog number 353114) and CD11c (red, catalog number 561352) |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD11c (cyan, catalog number 312204) and CD68 (red, catalog number sc-20060) |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 555392 | PE | AB_395793 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 563026 | BV510 | AB_2737960 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117314 | AF647 | AB_492850 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | NA |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 562561 | BV421 | AB_2737656 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20702 | Primary Antibody | CD11c | Human | Mouse | IgG1 | 3.9 | BioLegend | 301603 | FITC | AB_314173 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561355 | FITC | AB_10611872 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117308 | PE | AB_313777 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD11c (red, catalog number 117312) and CD64 (cyan, catalog number 139322), Mouse lymph node: PD-1 (cyan, catalog number 135218) and CD11c (magenta, catalog number 117312) |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_492850 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: CD11c (cyan, catalog number 117312) and CD11b (yellow, catalog number 101217) |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117371 | Spark Red 718 | AB_2924457 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 | NA |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | Thermo Fisher Scientific | MCD11c20 | AF488 | AB_10373244 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD11c (grey, catalog number MCD11c20) |
| P20702 | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | Abcam | ab216655 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117346 | AF594 | AB_2563323 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20702 | Primary Antibody | CD11c | Human | Mouse | BALB/c IgG1 | B-Ly6 | BD Biosciences | 561352 | AF700 | AB_10612006 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: CD8 (blue, catalog number 100738) and CD11c (magenta, catalog number 117312) |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse spleen: CD11c (cyan, catalog number 117312) and CD68 (red, catalog number 137017), Mouse Spleen Hochest (Blue) CD11c (Magenta Biolegend #117314) |
| P20702 | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | Abcam | ab216655 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lung: CD11c (magenta, catalog number 117312), CD206 (blue, catalog number 141717), and CD31 (green, catalog number 102520) |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9QXH4 | Primary Antibody | CD11c | Mouse | Armenian Hamster | IgG | N418 | BioLegend | 117312 | AF647 | AB_389328 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| P20702 | Primary Antibody | CD11c | Human | Rabbit | IgG | EP1347Y | Abcam | ab216655 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BioLegend | 142526 | AF647 | AB_2566239 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356517 | BV510 | AB_2562661 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BD Biosciences | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BD Biosciences | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD138 (red, catalog number 356504) and aSMA (cyan, catalog number 41-9760-82) |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356524 | AF647 | AB_2564250 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human skin: CD117 (magenta, catalog number 313203), CD138 (cyan, catalog number 356524), and Vimentin (blue, catalog number 677804) |
| P18828 | Primary Antibody | CD138 | Mouse | Rat | IgG2a | 281-2 | BD Biosciences | 562610 | BV421 | AB_11153126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356524 | AF647 | AB_2564250 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node metastatic: CD8 (green, catalog number 344716) and CD138 (blue, catalog number 356524) |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD138 (cyan, catalog number 356504) and IRF4 (red, catalog number 646404), Human lymph node: CD31 (magenta, catalog number 303133), CD138 (blue, catalog number 356504), and IRF4 (green, catalog number 646404 ) |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human liver: CD54 (cyan, catalog number 353114) and CD138 (purple, catalog number 356504) |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | BioLegend | 356504 | PE | AB_2561878 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: Beta-tubulin 3 (yellow, catalog number 801203) and CD138 (purple, catalog number 356504) |
| P18827 | Primary Antibody | CD138 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2780 | Unconjugated | AB_442186 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18827 | Primary Antibody | CD138 | Human | Mouse | IgG1 | MI15 | AAT Bioquest | 113800C0 | iF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08571 | Primary Antibody | CD14 | Human | Rabbit | IgG | EPR3653 | Abcam | ab226121 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P08571 | Primary Antibody | CD14 | Human | Rabbit | IgG | EPR3653 | Abcam | ab226121 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P08571 | Primary Antibody | CD14 | Human | Mouse | IgG1 | 61D3 | Thermo Fisher Scientific | 58-0149-41 | AF532 | AB_11218093 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P33151 | Primary Antibody | CD144 (VE-Cadherin) | Human | Mouse | IgG2a | BV9 | BioLegend | 348513 | AF647 | AB_2563669 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BioLegend | 394702 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BioLegend | 394702 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1% PFA Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD15 (cyan, catalog number 347420 and 715-545-020) and Vimentin (red, based on catalog number 677802) |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BioLegend | 394702 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P22083 | Primary Antibody | CD15 | Human | Mouse | IgM | MMA | BD Biosciences | 347420 | Unconjugated | AB_400298 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| A0A0B4J1G0 | Primary Antibody | CD16.2 | Mouse | Armenian Hamster | IgG | 9E9 | BioLegend | 149503 | PE | AB_2565810 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P08101 | Primary Antibody | CD16/32 | Mouse | Goat | IgG | Polyclonal | R&D Systems | AF1460-SP | Unconjugated | NA | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333602 (Unconjugated) | AF532 | AB_2892759 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human liver: CD163 (yellow, based on catalog number 333602) and CD49a (blue, catalog number 328303) |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | Thermo Fisher Scientific | MA5-11458 | Unconjugated | AB_10982556 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | Thermo Fisher Scientific | MA5-11458 | Unconjugated | AB_10982556 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| F7DAU3 | Primary Antibody | CD163 | Rhesus Macaque | Mouse | IgG2b | EDHu-1 | Novus Biologicals | NB110-40686AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333602 (Unconjugated) | AF532 | AB_2892759 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD163 (yellow, based on catalog number 333602) and CD4 (cyan, catalog number 300520), Human spleen: CD163 (yellow, based on catalog number 333602) and CD31 (blue, catalog number 303106) |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | EDHu-1 | Novus Biologicals | NB110-40686AF647 | AF647 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD163 (magenta, catalog number 333620) and CD20 (cyan, catalog number 53-0202-82) |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333611 | BV421 | AB_2562462 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333628 | BV510 | AB_2650632 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | Thermo Fisher Scientific | 12-1639-41 | PE | AB_1963571 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | 10D6 | Thermo Fisher Scientific | MA5-11458 | Unconjugated | AB_10982556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG1 | GHI/61 | BioLegend | 333620 | AF647 | AB_2563475 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD163 (red, catalog number 333620) and CD4 (blue, catalog number 300519) |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG2b | 6D3.2F3 | Novus Biologicals | NBP2-36495AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q86VB7 | Primary Antibody | CD163 | Human | Mouse | IgG2b | EPR19518 | Abcam | ab213612 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q13740 | Primary Antibody | CD166 | Human | Rabbit | IgG | EPR2759 | Abcam | ab197543 | AF488 | AB_2889210 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q13740 | Primary Antibody | CD166 | Human | Mouse | IgG1 | 3A6 | BioLegend | 343903 | PE | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD169 (green, catalog number 142404) and Ki-67 (magenta, catalog number 561277) |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | Bio-Rad | MCA884F | FITC | AB_324246 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142416 | AF594 | AB_2565620 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q62230 | Primary Antibody | CD169 | Mouse | Rat | IgG2a | 3D6.112 | BioLegend | 142404 | PE | AB_10915697 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse spleen: CD169 (green, catalog number 142404) and CD45 (blue, catalog number 103122) |
| P49682 | Primary Antibody | CD183 (CXCR3) | Human | Mouse | IgG1 | G025H7 | BioLegend | 353711 | AF647 | AB_10962946 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P49682 | Primary Antibody | CD183 (CXCR3) | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-16044 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P49682 | Primary Antibody | CD183 (CXCR3) | Human | Rabbit | IgG | Polyclonal | Abcam | ab154033 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 558112 | AF488 | AB_397034 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P15391 | Primary Antibody | CD19 | Human | Mouse | IgG1 | SJ25C1 | Thermo Fisher Scientific | 58-0198-41 | AF532 | AB_11219076 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P25918 | Primary Antibody | CD19 | Mouse | Rat | IgG2a | 6D5 | BioLegend | 115552 | AF594 | AB_2563459 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | AAT Bioquest | 10013150 | AF555 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331510 | AF647 | AB_1186032 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331522 | AF488 | AB_10720182 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331506 | PE | AB_1088999 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331506 | PE | AB_1088999 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD1c (red, catalog number 331506), CD8 (blue, catalog number 344716), and CD25 (yellow, catalog number 356128), Human lymph node: CD1c (yellow, catalog number 331506) and BCL6 (cyan, catalog number 561525) |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331525 | BV421 | AB_10933249 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P29017 | Primary Antibody | CD1c | Human | Mouse | IgG1 | L161 | BioLegend | 331529 | AF700 | AB_2563656 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11609 | Primary Antibody | CD1d | Mouse | Rat | IgG2b | 1B1 | BD Biosciences | 553846 | PE | AB_2073521 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | Yes | 0000-0002-6604-2065 | NA | 0000-0002-6604-2065 | Human tonsil FFPE: CD20 (red, catalog number 42-0202-82) |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 | NHP spleen: CD20 (magenta, catalog number 50-0202-82) and CD49a (cyan, catalog number ab243032 and A-31570) |
| Q3C2E2 | Primary Antibody | CD20 | Canine | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | NA |
| Q3C2E2 | Primary Antibody | CD20 | Canine | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine lymph node FFPE: CD20 (red, catalog number 42-0202-82) |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2b | 2H7 | BioLegend | 302322 | AF700 | AB_493753 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2b | 2H7 | BioLegend | 302354 | AF594 | AB_2565619 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Novus Biologicals | NBP2-80486AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 50-0202-82 | eF660 | AB_11150959 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P19437 | Primary Antibody | CD20 | Mouse | Rat | IgG2b | SA275A11 | BioLegend | 150432 | AF488 | AB_2890699 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | NA |
| H9YXP1 | Primary Antibody | CD20 | Rhesus Macaque | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 42-0202-82 | eF615 | AB_10853517 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Novus Biologicals | NBP2-80486AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD163 (magenta, catalog number 333620) and CD20 (cyan, catalog number 53-0202-82) |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD20 (purple, catalog number 53-0202-82) and Lumican (cyan, based on catalog number AF2846) |
| P11836 | Primary Antibody | CD20 | Human | Mouse | IgG2a | L26 | Thermo Fisher Scientific | 53-0202-82 | AF488 | AB_10734358 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9ES57 | Primary Antibody | CD200 | Mouse | Rat | IgG2a | OX-90 | BD Biosciences | 565547 | BV421 | AB_2739289 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD200 (grey, catalog number 565547) |
| P22897 | Primary Antibody | CD206 | Human | Mouse | IgG1 | 15-2 | BioLegend | 321130 | PE/Dazzle AF594 | AB_2616867 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141712 | AF647 | AB_10900420 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141710 | AF488 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: CD206 (yellow, catalog number 141710) and CD3 (white, catalog number 557869) |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141709 | AF488 | AB_10933252 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 | NA |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: CD206 (cyan, catalog number 141717) and Cytokeratin (purple, catalog number 628604) |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lung: CD11c (magenta, catalog number 117312), CD206 (blue, catalog number 141717), and CD31 (green, catalog number 102520) |
| P22897 | Primary Antibody | CD206 | Human | Mouse | IgG2b | 685645 | R&D Systems | MAB25341 | Unconjugated | AB_10890782 | Stock | Multiplexed 2D Imaging | FFPE | Lung | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q61830 | Primary Antibody | CD206 | Mouse | Rat | IgG2a | C068C2 | BioLegend | 141717 | BV421 | AB_2562232 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD206 (red, catalog number 141717) and CD106 (cyan, catalog number 105710) |
| Q8VBX4 | Primary Antibody | CD207 | Mouse | Rat | IgG2a | eBioL31 | Thermo Fisher Scientific | 12-2075-80 | PE | AB_763453 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | 1F8 | Novus Biologicals | NBP1-22527 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab240987 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | CR2/1952 | Novus Biologicals | NBP2-60733AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE : CD21 (yellow, catalog number ab306325) Antigen retrieval, pH 6 and pH 9 |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354903 | PE | AB_2561406 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354909 | FITC | AB_2561574 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab240987 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF488 | NA | Custom | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD21 (yellow, based on catalog number 354902) and CD44 (blue, catalog number 103016) |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | CD21 | Mouse | Rat | IgG2a | 7.E.9 | BioLegend | 123413 | Pacific Blue | AB_2085159 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD21 (purple, catalog number 123413) and Pax5 (cyan, catalog number 649704) |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD21 (red, based on catalog number 354902) and CD54 (blue, catalog number 353114), Human spleen: Ki-67 (yellow, catalog number 561277) and CD21 (cyan, based on catalog number 354902) |
| P20023 | Primary Antibody | CD21 | Human | Mouse | IgG1 | Bu32 | BioLegend | 354902 (Unconjugated) | AF532 | AB_2892739 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD21 (magenta, based on catalog number 354902) and CD44 (cyan, based on catalog number 103002) |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human normal LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) |
| P20023 | Primary Antibody | CD21 | Human | Rabbit | IgG | SP186 | Abcam | ab306325 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE : CD21 (yellow, catalog number ab306325) Antigen retrieval, pH 6 |
| P20693 | Primary Antibody | CD23 | Mouse | Rat | IgG2a | B3B4 | BioLegend | 101611 | AF647 | AB_493479 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-8909-5039 | NA | 0000-0003-4379-8967 | Mouse lymph node: PD-1 (green, catalog number 135218), CD23 (magenta, catalog number 101611), and IgD (blue, catalog number 405718), Mouse (naive) lymph node: CD23 (grey, catalog number 101611), Mouse (immunized) lymph node: CD23 (grey, catalog number 101611) |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338521 | BV421 | AB_2562460 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338505 | FITC | AB_1501125 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD23 (yellow, based on catalog number 338502) and CD106 (blue, catalog number 305806), Human lymph node: CD23 (magenta, based on catalog number 338502) and Ki-67 (green, catalog number 561277) |
| P06734 | Primary Antibody | CD23 | Human | Mouse | IgG1 | EBVCS-5 | BioLegend | 338502 (Unconjugated) | AF532 | AB_2892740 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01589 | Primary Antibody | CD25 | Human | Mouse | IgG2b | CD25-4E3 | Thermo Fisher Scientific | 17-0257-42 | APC | AB_11218671 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01589 | Primary Antibody | CD25 | Human | Mouse | IgG1 | M-A251 | BioLegend | 356128 | AF647 | AB_2563588 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01589 | Primary Antibody | CD25 | Human | Mouse | IgG1 | M-A251 | BioLegend | 356128 | AF647 | AB_2563588 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD25 (cyan, catalog number 356128) and FOXP3 (yellow, catalog number 41-4777-82) |
| P01590 | Primary Antibody | CD25 | Mouse | Rat | IgG1 | PC61.5 | Thermo Fisher Scientific | 53-0251-82 | AF488 | AB_763472 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08138 | Primary Antibody | CD271 (NGFR5) | Human | Mouse | IgG1 | NGFR5 | Thermo Fisher Scientific | MA5-13311 | Biotin | AB_10982271 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08138 | Primary Antibody | CD271 (NGFR5) | Human | Mouse | IgG1 | NGRF5 | BioLegend | 818201 | Unconjugated | AB_2564818 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9EP73 | Primary Antibody | CD274 (PD-L1) | Mouse | Rat | IgG2b | MIH1 | BD Biosciences | 753201 | RY586 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0000-0002-0835-911X | NA | 0000-0002-0835-911X | NA |
| P09055 | Primary Antibody | CD29 | Mouse | Armenian Hamster | IgG | HMB1-1 | BioLegend | 102214 | AF647 | AB_492831 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300483 | Spark Violet 538 | AB_2890733 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300448 | BV510 | AB_2563468 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: CD3 (red, catalog number 300448), Collagen IV (white, catalog number ab6586 and A-21038), and EpCAM (blue, catalog number 324228) |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100281 | Spark Red 718 | AB_2924440 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100205 | PE | AB_312662 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD3 (grey, catalog number 100210) |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100234 | BV510 | AB_2562555 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100228 | BV421 | AB_2562553 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967, 0009-0006-9784-2694 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Skin | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: CD206 (yellow, catalog number 141710) and CD3 (white, catalog number 557869) |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | Thermo Fisher Scientific | 58-0032-80 | AF532 | AB_11220474 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: CD3 (cyan, catalog number 58-0032-80) and CD45 (yellow, catalog number 103128) |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EPR4426 | Abcam | ab208514 | AF555 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100281 | Spark Red 718 | AB_2924440 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-1130-1899 | NA | 0000-0003-1130-1899 | Mouse Spleen: CD3e (white, catalog number 100281) |
| G7NCB9 | Primary Antibody | CD3 | Rhesus Macaque | Mouse | IgG1 | F7.2.38 | Agilent | M7254 | Unconjugated | AB_2631163 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Thermo Fisher Scientific | 58-0038-42 | AF532 | AB_11218675 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Novus Biologicals | NB100-2726IR | DL755 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human normal LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human FL LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD3 (green, Abcam cat. no. ab208514) |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300416 | AF647 | AB_389332 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300446 | AF594 | AB_2563236 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD3 (purple, catalog number 300446) and DC-SIGN (cyan, catalog number 330112) |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300402 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300415 | AF488 | AB_389310 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300448 | BV510 | AB_2563468 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | AAT Bioquest | 10032060 | iF514 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300401 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100281 | Spark Red 718 | AB_2924440 | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300435 | BV570 | AB_10898117 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | CAL57 | Cell IDx | HI06B-005 | UT016 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD3 (red, catalog number 1053135) and CD8 (cyan, catalog number 344716) |
| P27597 | Primary Antibody | CD3 | Canine | Rabbit | IgG | SP7 | Abcam | ab16669 | Unconjugated | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine lymph node FFPE: CD3 (orange, catalog number ab16669 and A21432) |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300408 | PE | AB_314062 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300487 | Spark Blue 574 | AB_2904329 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | Caprico Biotechnologies | 1053135 | iF594 | AB_2892737 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: CD3 (catalog number 100240) |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100214 | Pacific Blue | AB_493645 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 571224 | RY610 | AB_3686342 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node |
| P07766 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300415 | AF488 | AB_389310 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100240 | AF594 | AB_2563427 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD3 (cyan, catalog number 100240) and MHCII (blue, catalog number 107631) |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 571224 | RY610 | AB_3686342 | Stock | Ce3D | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: 2D slice at the edge of a ~250um-thick 3D image, Immunized mouse lymph node: 2D slice at the centre of a ~250um-thick 3D image |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100210 | AF488 | AB_389301 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 571224 | RY610 | AB_3686342 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) but with signal maximally increased in Imaris to display the completeness of signal loss with dye inactivation., Immunized mouse lymph node: dye-inactivated section imaged maximized microscope settings (laser power, gain) and optimal Imaris setting for visualization to evaluate potential residual non-inactivated signal which might interfere with subsequent imaging cycles, especially of dim markers. |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | Thermo Fisher Scientific | 58-0032-80 | AF532 | AB_11220474 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| P22646 | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BD Biosciences | 557869 | AF647 | AB_396912 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | CD3 | Mouse | Rat | IgG2b | 17A2 | BioLegend | 100275 | Spark Blue 574 | AB_2904266 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102516 | AF647 | AB_2161029 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse gut: EpCAM (blue, catalog number 118222), CD31 (magenta, catalog number 102516), and MHCII (green, catalog number 107622) |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102514 | AF488 | AB_2161031 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD163 (yellow, based on catalog number 333602) and CD31 (blue, catalog number 303106) |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303133 | AF700 | AB_2566326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | Mouse lymph node: CD31 (grey, catalog number 102520) |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lung: Ly-6G (yellow, catalog number 127626), CD31 (blue, catalog number 102520), and CD8 (red, catalog number 100724) |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303110 | AF488 | AB_493074 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303102 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303126 | AF594 | AB_2563303 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303112 | AF647 | AB_493076 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | Novus Biologicals | NBP2-47785AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | Novus Biologicals | NBP2-47785AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BD Biosciences | 553373 | PE | AB_394819 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303133 | AF700 | AB_2566326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD31 (magenta, catalog number 303133), CD138 (blue, catalog number 356504), and IRF4 (green, catalog number 646404 ), Human lymph node: CD31 (red, catalog number 303133) and CD49a (cyan, catalog number 328303) |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102515 | AF647 | AB_2161030 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | Mouse Brain Tumor Hochest(Blue) mCherry (Green Novus #NBP2-25158) CD31(Magenta Biolegend #102515) |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102516 | AF647 | AB_2161029 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD4 (cyan, catalog number 300520) and CD31 (magenta, catalog number 303106) |
| Q08481 | Primary Antibody | CD31 | Mouse | Rat | IgG2a | MEC13.3 | BioLegend | 102520 | AF594 | AB_2563319 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: Hoechst (blue, catalog number 40046), CD106 (cyan, catalog number 305806), and CD31 (red, catalog number 303106) |
| A0A5F7Z8X0 | Primary Antibody | CD31 | Rhesus Macaque | Rabbit | IgG | Polyclonal | Abcam | ab28364 | Unconjugated | AB_726362 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | C31.3+C31.7+C31.10 | Novus Biologicals | NBP2-47785AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16284 | Primary Antibody | CD31 | Human | Mouse | IgG1 | WM59 | BioLegend | 303106 | PE | AB_314332 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0009-0006-9784-2694 | NA | 0009-0006-9784-2694 | Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD34 (green, Thermo Fisher Scientific cat. no. MA1-10205) |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Novus Biologicals | FAB7227T | AF594 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Novus Biologicals | FAB7227T | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | 4H11 | AAT Bioquest | 103400C0 | iF594 | AB_2892741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333404 | FITC | AB_2085022 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | CD35 | Mouse | Rat | IgG2a | 8C12 | BD Biosciences | 740132 | BV510 | AB_2739889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD35 (yellow, catalog number 740132) and Collagen IV (blue, catalog number ab19808 and A-11009), Mouse (immunized) lymph node: CD35 (green, catalog number 740132) and DAPI (blue, catalog number 62248) |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BD Biosciences | 565329 | AF647 | AB_2739186 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333406 | PE | AB_2292231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333406 | PE | AB_2292231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD35 (blue, catalog number 333406), CD117 (yellow, catalog number 313234), and CD66b (magenta, catalog number 305110), Human lymph node: CD39 (red, catalog number 328208) and CD35 (cyan, catalog number 333406), Human lymph node: ICOS (yellow, catalog number 313514) and CD35 (purple, catalog number 333406) |
| P17927 | Primary Antibody | CD35 | Human | Mouse | IgG1 | E11 | BioLegend | 333406 | PE | AB_2292231 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P56528 | Primary Antibody | CD38 | Mouse | Rat | IgG2a | 90 | BioLegend | 102732 | BV421 | AB_2734153 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | NA |
| P28907 | Primary Antibody | CD38 | Human | Mouse | IgG1 | HIT2 | BioLegend | 303503 | FITC | AB_314355 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P28907 | Primary Antibody | CD38 | Human | Mouse | IgG1 | HIT2 | BioLegend | 303524 | AF700 | AB_2072781 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD38 (blue, catalog number 303524) and Clec9a (magenta, based on catalog number 353802) |
| P55772 | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | BioLegend | 328208 | PE | AB_940429 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD117 (cyan, catalog number 313234), CD39 (blue, catalog number 328208), and CD94 (magenta, catalog number 305506), Human lymph node: CD39 (red, catalog number 328208) and CD35 (cyan, catalog number 333406) |
| P55772 | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | BioLegend | 328205 | FITC | AB_940423 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P55772 | Primary Antibody | CD39 | Human | Mouse | IgG1 | A1 | BioLegend | 328205 | FITC | AB_940423 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P22646 | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | Bio-Rad | MCA463SBV710 | StarBright Violet 710 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X | NA |
| P22646 | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | Bio-Rad | MCA463SBB700 | StarBright Blue 700 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X | NA |
| P22646 | Primary Antibody | CD3e | Human | Mouse | IgG1 | UCHT1 | Bio-Rad | MCA463SBV670 | StarBright Violet 670 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-0835-911X | NA | 0000-0002-0835-911X | NA |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | Thermo Fisher Scientific | 58-0049-42 | AF532 | AB_2802361 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD4 (cyan, catalog number 58-0049-42) and CD8 (red, catalog number 561525) |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD4 (cyan, catalog number 300520) and CD31 (magenta, catalog number 303106) |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 41-0042-82 | eF570 | AB_2573595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P16003 | Primary Antibody | CD4 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300519 | AF488 | AB_389311 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD163 (red, catalog number 333620) and CD4 (blue, catalog number 300519) |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300526 | AF700 | AB_493743 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node metastatic: CD4 (blue, catalog number 300526) and EpCAM (red, catalog number 324228) |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BD Biosciences | 568161 | RY586 | AB_3684090 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100443 | BV421 | AB_2562557 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | Mouse lymph node: CD4 (grey, catalog number 100449) |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | Novus Biologicals | NBP2-27216AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 58-0042-80 | AF532 | AB_11219484 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 58-0042-80 | AF532 | AB_11219484 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16003 | Primary Antibody | CD4 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | FAB8165N | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BD Biosciences | 572205 | RY743 | AB_3686786 | Stock | Ce3D | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: 2D slice at the edge of a ~250um-thick 3D image, Immunized mouse lymph node: 2D slice at the centre of a ~250um-thick 3D image |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | Thermo Fisher Scientific | 41-0042-82 | eF570 | AB_2573595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BD Biosciences | 557667 | AF488 | AB_396779 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BD Biosciences | 553730 | PE | AB_396634 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD4 (cyan, catalog number 300520) and Vimentin (red, based on catalog number 677802), Human spleen: CD163 (yellow, based on catalog number 333602) and CD4 (cyan, catalog number 300520) |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | Mouse Brain Tumor Hochest (Blue) mCherry (green, Novus #NBP2-25158 ) CD4 (Magenta, Biolegend #100533) |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | GK1.5 | BioLegend | 100446 | AF594 | AB_2563182 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse spleen: CD4 (cyan, catalog number 100446), CD8 (magenta, catalog number 100738), and IgD (blue, catalog number 405730) |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | AAT Bioquest | 10041090 | iF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | AF-379-SP | Unconjugated | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | FAB8165G | AF488 | AB_2728839 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | Yes | 0000-0002-6604-2065 | NA | 0000-0002-6604-2065 | Human tonsil FFPE: CD4 (green, catalog number FAB8165G) |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA1211 | Miltenyi Biotec | 130-123-209 | VioGreen | AB_2811462 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA1211 | Miltenyi Biotec | 130-128-234 | Vio Bright R720 | AB_2905066 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-118-568 | VioBlue | AB_2733943 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-118-794 | PerCP-Vio 700 | AB_2733032 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BD Biosciences | 572205 | RY743 | AB_3686786 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) but with signal maximally increased in Imaris to display the completeness of signal loss with dye inactivation., Immunized mouse lymph node: dye-inactivated section imaged maximized microscope settings (laser power, gain) and optimal Imaris setting for visualization to evaluate potential residual non-inactivated signal which might interfere with subsequent imaging cycles, especially of dim markers. |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-118-316 | PE-Vio 615 | AB_2728077 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 | NA |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human normal LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-119-132 | APC-Vio 770 | AB_2733118 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BD Biosciences | 557667 | AF488 | AB_396779 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | 0000-0002-1461-0999 | 0000-0003-4379-8967 | Mouse lymph node: CD4 (grey, catalog number 100443) |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human FL LN: BCL2 (cyan, catalog number ab236221 and A-31573), CD3 (magenta, catalog number ab208514), CD4 (blue, catalog number ab196372), CD21 (yellow, catalog number ab306325) |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BD Biosciences | 568161 | RY586 | AB_3684090 | Stock | Ce3D | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: 2D slice at the edge of a ~250um-thick 3D image, Immunized mouse lymph node: 2D slice at the centre of a ~250um-thick 3D image |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Abcam | ab196372 | AF488 | AB_2889191 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2a | RM4-5 | BioLegend | 100533 | AF647 | AB_493372 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Spleen | Healthy | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | Mouse Spleen Hochest (Blue) CD4 (Magenta Biolegend #100533) |
| P06332 | Primary Antibody | CD4 | Mouse | Human | IgG1 | REA604 | Miltenyi Biotec | 130-123-894 | PE-Vio 770 | AB_2811571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-6980-8900 | NA | 0000-0002-6980-8900 | NA |
| P01730 | Primary Antibody | CD4 | Human | Rabbit | IgG | EPR6855 | Cell IDx | HI06B-005 | UT019 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01730 | Primary Antibody | CD4 | Human | Goat | IgG | Polyclonal | R&D Systems | AF-379-SP | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BD Biosciences | 568161 | RY586 | AB_3684090 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) and identical Imaris channel settings to the pre-inactivation image to determine susceptibility to signal loss with dye inactivation., Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) but with signal maximally increased in Imaris to display the completeness of signal loss with dye inactivation. |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | Thermo Fisher Scientific | 58-0049-42 | AF532 | AB_2802361 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BD Biosciences | 572205 | RY743 | AB_3686786 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | No | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node |
| NA | Primary Antibody | CD4 | Canine | Rat | IgG2a | YKIX302.9 | Thermo Fisher Scientific | MA5-28357 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine lymph node FFPE: CD4 (magenta, catalog number MA5-28357 and A48272) |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100443 | BV421 | AB_2562557 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | Mouse liver: CD4 (yellow, catalog number 100443), CD11c (cyan, catalog number 117312), and CD11b (purple, catalog number 101217) |
| P01730 | Primary Antibody | CD4 | Human | Mouse | IgG1 | RPA-T4 | BioLegend | 300520 | AF647 | AB_389333 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD21 (yellow, based on catalog number 354902) and CD44 (blue, catalog number 103016) |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103054 | AF594 | AB_2563183 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD44 (catalog number 103018) |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103026 | AF700 | AB_493713 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | Mouse liver: CD8 (cyan, catalog number 100738), CD44 (blue, catalog number 103026), and NK1.1 (red, catalog number 108731) |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103074 | Spark YG 570 | AB_2892265 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103026 | AF700 | AB_493713 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | No | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | NA | IM7 | AAT Bioquest | 104410C1 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: CD8 (yellow, catalog number 100724) and CD44 (white, catalog number 104410C1) |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | AAT Bioquest | 104410C0 | iF594 | AB_2935685 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: CD44 (red, catalog number 104410C0) and Hoechst (blue, catalog number 40046) |
| P15379 | Primary Antibody | CD44 | Mouse | Rat | IgG2b | IM7 | BioLegend | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: CD44 (blue, based on catalog number 103002) and TCRgd (yellow, catalog number 118108) |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD21 (magenta, based on catalog number 354902) and CD44 (cyan, based on catalog number 103002) |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103007 | PE | AB_312958 | Stock | Multiplexed 2D Imaging | FFPE | Thymus | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD49a (cyan, catalog number 328310) and CD44 (red, catalog number 103018) |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103018 | AF647 | AB_493681 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human skin: Keratin 14 (yellow, catalog number 906004 and A-32932) and CD44 (purple, catalog number 103016) |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103016 | AF488 | AB_493679 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: EpCAM (blue, catalog number 324210) and CD44 (yellow, catalog number 103016) |
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103002 (Unconjugated) | AF532 | AB_2892768 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human liver: Cytokeratin 7 (green, catalog number 601606), CD44 (blue, based on catalog number 103002) and CD45 (magenta, catalog number 304060) |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 571962 | RB670 | AB_3686770 | Stock | Ce3D | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | No | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: 2D slice at the edge of a ~250um-thick 3D image, Immunized mouse lymph node: 2D slice at the centre of a ~250um-thick 3D image |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 569150 | RB780 | AB_3684818 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) and identical Imaris channel settings to the pre-inactivation image to determine susceptibility to signal loss with dye inactivation. |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11 + PD7/26 | Novus Biologicals | NBP2-34528AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE: CD45 (magenta, catalog number NBP2-34528AF700) |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 570291 | RB705 | AB_3086726 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) and identical Imaris channel settings to the pre-inactivation image to determine susceptibility to signal loss with dye inactivation., Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) but with signal maximally increased in Imaris to display the completeness of signal loss with dye inactivation., Immunized mouse lymph node: dye-inactivated section imaged maximized microscope settings (laser power, gain) and optimal Imaris setting for visualization to evaluate potential residual non-inactivated signal which might interfere with subsequent imaging cycles, especially of dim markers. |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 569150 | RB780 | AB_3684818 | Stock | Ce3D | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | No | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: 2D slice at the edge of a ~250um-thick 3D image, Immunized mouse lymph node: 2D slice at the centre of a ~250um-thick 3D image |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 570291 | RB705 | AB_3086726 | Stock | Ce3D | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: 2D slice at the edge of a ~250um-thick 3D image, Immunized mouse lymph node: 2D slice at the centre of a ~250um-thick 3D image |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528PE | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0009-0006-9784-2694 | NA | 0009-0006-9784-2694 | Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD45 (green, Novus Biologicals cat. no. NBP2-34528PE) |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 571481 | RB613 | AB_3686553 | Stock | Ce3D | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | No | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: 2D slice at the edge of a ~250um-thick 3D image, Immunized mouse lymph node: 2D slice at the centre of a ~250um-thick 3D image |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0009-0006-9784-2694 | NA | 0009-0006-9784-2694 | Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD45 (green, Novus Biologicals cat. no. NBP2-34528AF488) |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 571472 | RY703 | AB_3686545 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) and identical Imaris channel settings to the pre-inactivation image to determine susceptibility to signal loss with dye inactivation., Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) but with signal maximally increased in Imaris to display the completeness of signal loss with dye inactivation., Immunized mouse lymph node: dye-inactivated section imaged maximized microscope settings (laser power) and optimal Imaris setting for visualization to evaluate potential residual non-inactivated signal which might interfere with subsequent imaging cycles, especially of dim markers. |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 571472 | RY703 | AB_3686545 | Stock | Ce3D | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: 2D slice at the edge of a ~250um-thick 3D image, Immunized mouse lymph node: 2D slice at the centre of a ~250um-thick 3D image |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016184 | PE/iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 571962 | RB670 | AB_3686770 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) but with signal maximally increased in Imaris to display the completeness of signal loss with dye inactivation., Immunized mouse lymph node: dye-inactivated section imaged maximized microscope settings (laser power, gain) and optimal Imaris setting for visualization to evaluate potential residual non-inactivated signal which might interfere with subsequent imaging cycles, especially of dim markers. |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 571481 | RB613 | AB_3686553 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) but with signal maximally increased in Imaris to display the completeness of signal loss with dye inactivation., Immunized mouse lymph node: dye-inactivated section imaged maximized microscope settings (laser power, gain) and optimal Imaris setting for visualization to evaluate potential residual non-inactivated signal which might interfere with subsequent imaging cycles, especially of dim markers. |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 569150 | RB780 | AB_3684818 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | No | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Uterus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2634-6838 | NA | 0000-0003-2634-6838 | NA |
| NA | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103183 | Spark Blue 574 | AB_2904274 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| NA | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103183 | Spark Blue 574 | AB_2904274 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | No | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: CD3 (cyan, catalog number 58-0032-80) and CD45 (yellow, catalog number 103128) |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103134 | BV421 | AB_2562559 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Novus Biologicals | NB100-77417IR | DL755 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-9189-7172 | NA | 0000-0001-9189-7172 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103172 | Spark YG 570 | AB_2860598 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse LN: CD45 (orange, catalog number 103172) and Hoechst (blue, catalog number 40046) |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | Thermo Fisher Scientific | 58-0459-41 | AF532 | AB_11218084 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: CD45 (cyan, catalog number 58-0459-41) and EpCAM (blue, catalog number 324228) |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Infected | Akoya Antibody Diluent | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-9189-7172 | NA | 0000-0001-9189-7172 | NA |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | BioLegend | 304018 | AF647 | AB_389336 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103122 | AF488 | AB_493531 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103138 | BV510 | AB_2563061 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103134 | BV421 | AB_2562559 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103144 | AF594 | AB_2563458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | Mouse liver: E-Cadherin (red, catalog number 147308), and CD45 (cyan, catalog number 103128) |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 571481 | RB613 | AB_3686553 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 571962 | RB670 | AB_3686770 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 571472 | RY703 | AB_3686545 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016184 | PE/iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528PE | PE | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103144 | AF594 | AB_2563458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | Mouse lymph node: CD45 (grey, catalog number 103144) |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | Thermo Fisher Scientific | 58-0459-41 | AF532 | AB_11218084 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | Thermo Fisher Scientific | 58-0451-82 | AF532 | AB_11218871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103124 | AF647 | AB_493533 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103128 | AF700 | AB_493715 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103124 | AF647 | AB_493533 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD45 (catalog number 103124) |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | HI30 | BioLegend | 304060 | AF594 | AB_2629599 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BD Biosciences | 570291 | RB705 | AB_3086726 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1X BD PermWash Buffer | NA | NA | Yes | 0000-0002-6863-1461 | NA | 0000-0002-6863-1461 | Immunized mouse lymph node |
| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG2a | F10-89-4 | Caprico Biotechnologies | 1016136 | iF594 | AB_2892743 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD45 (blue, catalog number 1016136) and CD31 (magenta, catalog number 303106) |
| P06800 | Primary Antibody | CD45 | Mouse | Rat | IgG2b | 30-F11 | BioLegend | 103122 | AF488 | AB_493531 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse spleen: CD169 (green, catalog number 142404) and CD45 (blue, catalog number 103122) |
| NA | Primary Antibody | CD45.1 | Mouse | Mouse | IgG2a | A20 | BioLegend | 110718 | AF488 | AB_492862 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-8909-5039 | NA | 0000-0002-8909-5039 | Mouse (immunized) lymph node: CD45.1 (magenta, catalog number 110718) and CD138 (yellow, catalog number 142526) |
| NA | Primary Antibody | CD45.2 | Mouse | Mouse | IgG2a | 104 | BioLegend | 109850 | AF594 | AB_2629589 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| NA | Primary Antibody | CD45.2 | Mouse | Mouse | IgG2a | 104 | BioLegend | 109818 | AF647 | AB_492870 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| NA | Primary Antibody | CD45RA | Human | Mouse | IgG1 | 5H9 | BD Biosciences | 556626 | FITC | AB_396498 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | CL7207 | Abcam | ab243032 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328303 | PE | AB_1236441 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD49a (cyan, catalog number 328303) and CD31 (red, catalog number 303133) |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328308 | FITC | AB_2129084 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | CL7207 | Abcam | ab243032 | Unconjugated | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328303 | PE | AB_1236407 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | Human liver: CD163 (yellow, based on catalog number 333602) and CD49a (blue, catalog number 328303) |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD49a (green, catalog number 328310) and Vimentin (blue, catalog number 677804) |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: EpCAM (purple, catalog number 324210) and CD49a (cyan, catalog number 328310) |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD49a (cyan, catalog number 328310) and CD44 (red, catalog number 103018), Human spleen: CD49a (cyan, catalog number 328310) and Fibrinogen (red, catalog number ab118533 and A-21102) |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: CD49a (sea green, catalog number 328310) and Hoechst (blue, catalog number 40046) |
| NA | Primary Antibody | CD49a | Rhesus Macaque | Mouse | IgG1 | CL7207 | Abcam | ab243032 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 | NHP spleen: CD20 (magenta, catalog number 50-0202-82) and CD49a (cyan, catalog number ab243032 and A-31570) |
| P56199 | Primary Antibody | CD49a | Human | Mouse | IgG1 | TS2/7 | BioLegend | 328310 | AF647 | AB_2129242 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD21 (red, based on catalog number 354902) and CD54 (blue, catalog number 353114) |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353125 | AF700 | AB_2810560 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human liver: CD54 (cyan, catalog number 353114) and CD138 (purple, catalog number 356504) |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353114 | AF647 | AB_2715942 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: Hoechst (blue, catalog number 40046), CD54 (green, catalog number 353114), and Vimentin (magenta, catalog number 677807) |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3.D5 | Novus Biologicals | NBP2-33132AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | BioLegend | 304603 | FITC | AB_314445 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3 | Bio-Rad | MCA2693A488T | AF488 | AB_1102209 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | BioLegend | 304605 | PE | AB_314447 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Ileum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG1 | 123C3.D5 | Novus Biologicals | NBP2-33132AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P13591 | Primary Antibody | CD56 | Human | Mouse | IgG2a | MEM-188 | BioLegend | 304611 | AF488 | AB_493179 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| F7DRX3 | Primary Antibody | CD57 | Human | Mouse | IgM | HNK-1 | BioLegend | 359602 | Unconjugated | AB_2562403 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| F7DRX3 | Primary Antibody | CD57 | Human | Mouse | IgM | HNK-1 | BioLegend | 359602 | Unconjugated | AB_2562403 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P05106 | Primary Antibody | CD61 | Human | Mouse | IgG1 | Y2/51 | Miltenyi Biotec | 130-098-683 | FITC | AB_2660493 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | Human spleen: CD163 (magenta, based on catalog number 333602) and CD61 (green, catalog number 130-098-683) |
| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139322 | AF647 | AB_2566561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD11c (red, catalog number 117312) and CD64 (cyan, catalog number 139322), Mouse lymph node: CD64 (grey, catalog number 139322) |
| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139322 | AF647 | AB_2566561 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P26151 | Primary Antibody | CD64 | Mouse | Mouse | IgG1 | X54-5/7.1 | BioLegend | 139304 | PE | AB_10612740 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P13688 | Primary Antibody | CD66abce | Rhesus Macaque | Mouse | IgG2b | Tet2 | Miltenyi Biotec | 130-116-522 | FITC | AB_2727588 | Stock | Opal-plex | 10% Formalin for 7 Days | Lung | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| P31997 | Primary Antibody | CD66b | Human | Mouse | IgM | G10F5 | Caprico Biotechnologies | 1075136 | iF594 | AB_2892744 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P31997 | Primary Antibody | CD66b | Human | Mouse | IgM | G10F5 | BioLegend | 305110 | AF647 | AB_2563171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 AF647 | AF647 | AB_3073741 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 AF647 | AF647 | AB_3073741 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 AF647 | AF647 | AB_3073741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | NA |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 | NA |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | NA |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Uterus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2634-6838 | NA | 0000-0003-2634-6838 | NA |
| A0A8C0Q9Z4 | Primary Antibody | CD68 | Canine | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine melanoma skin FFPE: CD68 (magenta but no signal, catalog number 1064136), pan-cytokeratin (green, catalog number 53-9003-80), and Hoechst (blue, catalog number 40046) |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Santa Cruz Biotechnology | sc-20060 AF647 | AF647 | AB_3073741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137004 | AF647 | AB_2044002 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse spleen: CD11c (cyan, catalog number 117312) and CD68 (red, catalog number 137017) |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD44 (cyan, catalog number X) and CD68 (magenta, catalog number 1064135) |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: F4/80 (cyan, catalog number 123132), CD68 (blue, catalog number 137017) and NK1.1 (magenta, catalog number 108731) |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137011 | AF488 | AB_2074847 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: CD68 (cyan, catalog number 137017) and MHCII (red, catalog number 107622) |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lung: Beta-tubulin 3 (yellow, based on catalog number 657402), CD68 (cyan, catalog number 137017), and CD44 (magenta, catalog number 103018) |
| P34810 | Primary Antibody | CD68 | Human | Mouse | IgG1 | KP1 | Caprico Biotechnologies | 1064135 | iF594 | AB_2892745 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | BioLegend | 310918 | AF647 | AB_528871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: HLA-DR (cyan, catalog number 307620) and CD69 (red, catalog number 310918), Human lymph node: CD69 (red, catalog number 310918) and CD44 (cyan, catalog number 103018) |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | BioLegend | 310918 | AF647 | AB_528871 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG1 | FN50 | BioLegend | 310906 | PE | AB_314841 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3218-4479 | NA | 0000-0002-3218-4479 | NA |
| Q07108 | Primary Antibody | CD69 | Human | Mouse | IgG2a | 15B5G2 | Novus Biologicals | NBP2-25236AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q61503 | Primary Antibody | CD73 | Mouse | Rat | IgG1 | TY/11.8 | BioLegend | 127230 | AF700 | AB_2800625 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-2634-6838 | NA | 0000-0003-2634-6838 | NA |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD8 (cyan, catalog number 100738), DEC205 (purple, catalog number 138204), and CD169 (yellow, catalog number 142404) |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | Mouse liver: CD8 (cyan, catalog number 100738), CD44 (blue, catalog number 103026), and NK1.1 (red, catalog number 108731) |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD1c (red, catalog number 331506), CD8 (blue, catalog number 344716), and CD25 (yellow, catalog number 356128), Human lymph node: CD4 (cyan, catalog number 58-0049-42) and CD8 (red, catalog number 561525) |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: CD8 (blue, catalog number 100738) and CD11c (magenta, catalog number 117312) |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | Thermo Fisher Scientific | 14-0085-82 | Unconjugated | AB_11150240 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | AAT Bioquest | 100810C0 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | RPA-T8 | BD Biosciences | 557945 | AF700 | AB_396953 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372910 | Spark YG 570 | AB_2904405 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | RPA-T8 | Thermo Fisher Scientific | 58-0088-24 | AF532 | AB_11220473 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse spleen: CD4 (cyan, catalog number 100446), CD8 (magenta, catalog number 100738), and IgD (blue, catalog number 405730) |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 | Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD8 (red, BioLegend cat. no. 372906) |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100724 | AF647 | AB_389326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: CD8 (yellow, catalog number 100724) and CD44 (white, catalog number 104410C1) |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | CD8 | Canine | Rat | IgG1 | YCATE55.9 | Thermo Fisher Scientific | MA5-28430 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | NA |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100752 | BV510 | AB_2563057 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BD Biosciences | 553032 | PE | AB_394571 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100723 | AF488 | AB_389304 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967, 0009-0006-9784-2694 | NA | 0000-0003-4379-8967 | NA |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BD Biosciences | 553032 | PE | AB_394571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100758 | AF594 | AB_2563237 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01732 | Primary Antibody | CD8 | Human | Rabbit | IgG | EPR10640 | Cell IDx | HI06B-005 | UT015 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100724 | AF647 | AB_389326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Cervix | Cervical Cancer | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Skin | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node metastatic: CD8 (green, catalog number 344716) and CD138 (blue, catalog number 356524) |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse gut: EpCAM (magenta, catalog number 118222), CD8 (blue, catalog number 100738), and CD31 (green, catalog number 102516) |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD8 (cyan, catalog number 344716) and CD3 (red, catalog number 1053135) |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Heart | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7586-9996 | NA | 0000-0002-7586-9996 | NA |
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | SK1 | BioLegend | 344716 | AF488 | AB_10549301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P33681 | Primary Antibody | CD80 | Human | Mouse | IgG2b | 62N3G8 | Novus Biologicals | NBP2-25255AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P33681 | Primary Antibody | CD80 | Human | Goat | IgG | Polyclonal | R&D Systems | AF140 | Unconjugated | AB_354778 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P33681 | Primary Antibody | CD80 | Human | Mouse | IgG1 | 2D10 | BioLegend | 305215 | AF647 | AB_528874 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P35762 | Primary Antibody | CD81 | Mouse | Armenian Hamster | IgG1 | Eat-2 | BioLegend | 104905 | PE | AB_2076267 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-8909-5039 | NA | 0000-0002-8909-5039 | Mouse lymph node: CD81 (grey, catalog number 104905) |
| Q01151 | Primary Antibody | CD83 | Human | Mouse | IgG1 | HB15e | BioLegend | 305314 | AF488 | AB_528877 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q01151 | Primary Antibody | CD83 | Human | Mouse | IgG1 | HB15e | BD Biosciences | 550634 | PE | AB_393794 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | CD86 | Mouse | Rat | IgG2a | GL-1 | BioLegend | 105020 | AF647 | AB_493465 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | No | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| P42081 | Primary Antibody | CD86 | Human | Goat | IgG | Polyclonal | R&D Systems | AF-141-NA | Unconjugated | AB_354385 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P42081 | Primary Antibody | CD86 | Human | Mouse | IgG2b | IT2.2 | BioLegend | 305415 | AF647 | AB_528882 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P42082 | Primary Antibody | CD86 (B7-2) | Mouse | Rat | IgG2a | GL1 | R&D Systems | FAB741S-100UG | AF750 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X | NA |
| P01732 | Primary Antibody | CD8a | Mouse | Mouse | IgG1 | HIT8a | BioLegend | 300908 | PE | AB_314112 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P01731 | Primary Antibody | CD8a | Mouse | Rat | IgG2a | 53-6.7 | R&D Systems | FAB116S-100UG | AF750 | NA | Stock | Ce3D | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | NA | No | 0000-0002-0835-911X | NA | 0000-0002-0835-911X | NA |
| P01831 | Primary Antibody | CD90.1 | Mouse | Mouse | IgG1 | OX-7 | BioLegend | 202508 | AF647 | AB_492884 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | NA |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305506 | AF488 | AB_314536 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD94 (cyan, catalog number 305506) and CD45 (purple, catalog number 1016185) |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305512 | AF647 | AB_2249691 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305506 | PE | AB_314536 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305506 | PE | AB_314536 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q13241 | Primary Antibody | CD94 | Human | Mouse | IgG1 | DX22 | BioLegend | 305504 | FITC | AB_314534 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9NXV6 | Primary Antibody | CDKN2a | Human | Mouse | IgG2a | ARF 4C6/4 | Santa Cruz Biotechnology | sc-53392 | Unconjugated | AB_630056 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9NXV6 | Primary Antibody | CDKN2a | Human | Rat | IgG2b | 5-C3-1 | Novus Biologicals | NB200-174AF647 | AF647 | AB_10002046 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P13569 | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | Thermo Fisher Scientific | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P13569 | Primary Antibody | CFTR | Human | Mouse | IgM | CF3 | Thermo Fisher Scientific | MA1-935 | Unconjugated | AB_2081230 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P13569 | Primary Antibody | CFTR | Human | Mouse | IgG1 | M3A7 | Thermo Fisher Scientific | MA5-11768 | Unconjugated | AB_10983660 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| NA | Secondary Antibody | Chicken IgY (H&L) | Zebrafish | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11039 | AF488 | AB_2534096 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Secondary Antibody | Chicken IgY (H&L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-32932 | AF555 | AB_2762844 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| NA | Secondary Antibody | Chicken IgY (H&L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA1-72000 | FITC | AB_923386 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P10645 | Primary Antibody | Chromogranin A | Human | Mouse | IgG1 | LK2H10 | Novus Biologicals | NBP2-34672AF532 | AF532 | AB_2892553 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P58304 | Primary Antibody | Chx10 | Mouse | Mouse | IgG2a | E-12 | Santa Cruz Biotechnology | sc-365519 AF546 | AF546 | AB_10842442 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Chx10 (cyan, catalog number sc-365519) and Hoechst (blue, catalog number 40046) |
| P70194 | Primary Antibody | CLEC4F | Mouse | Goat | IgG | Polyclonal | R&D Systems | AF2784 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| Q6UXN8 | Primary Antibody | CLEC9a | Human | Mouse | IgG2a | 8F9 | BioLegend | 353802 (Unconjugated) | AF488 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD38 (blue, catalog number 303524) and Clec9a (magenta, based on catalog number 353802) |
| Q9BWP8 | Primary Antibody | COLEC11 | Human | Rabbit | IgG | Polyclonal | Proteintech | 15269-1-AP | Unconjugated | AB_10732940 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P02462 | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | Abcam | ab6586 | Unconjugated | AB_305584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: Hoechst (blue, catalog number 40046) and Collagen IV (red, catalog number ab6586) |
| P02463 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02463 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02462 | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | Abcam | ab6586 | Unconjugated | AB_305584 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: CD3 (red, catalog number 300448), Collagen IV (white, catalog number ab6586 and A-21038), and EpCAM (blue, catalog number 324228) |
| P02463 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02462 | Primary Antibody | Collagen IV | Human | Rabbit | IgG | Polyclonal | Abcam | ab6586 | Unconjugated | AB_305584 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02463 | Primary Antibody | Collagen IV | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab19808 | Unconjugated | AB_445160 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 | NA |
| Q9EQP6 | Primary Antibody | Cone Arrestin | Mouse | Rabbit | IgG | Polyclonal | MilliporeSigma | AB15282 | Unconjugated | AB_1163387 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | NA |
| Q6PBM9 | Primary Antibody | Cone Arrestin | Zebrafish | Rabbit | IgG | Polyclonal | MilliporeSigma | AB15282 | Unconjugated | AB_1163387 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q6FHL8 | Primary Antibody | CTGF | Human | Rabbit | IgG | OT11F4 | FabGennix | CTGF-FITC | FITC | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE: CTGF (green, catalog number FITC-CTGF) |
| P16410 | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | Abcam | ab270127 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human FFPE follicular lymphoma lymph node: CTLA-4 (yellow, catalog number ab270127 and PD-1 (cyan, catalog number ab275126), Human FFPE follicular lymphoma lymph node: CTLA-4 (yellow, catalog number ab270127) |
| P16410 | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | Abcam | ab270127 | AF647 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16410 | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | Abcam | ab270127 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0009-0006-9784-2694 | NA | 0009-0006-9784-2694 | Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CTLA4 (red, Abcam cat. no. ab270127) |
| Q6ICW0 | Primary Antibody | CXCL12 | Human | Mouse | IgG1 | 79018 | R&D Systems | MAB350-500 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q6ICW0 | Primary Antibody | CXCL12 | Human | Mouse | IgG1 | 79018 | R&D Systems | MAB350 | Unconjugated | AB_2088149 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P63158 | Primary Antibody | CXCL12 | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab25117 | NA | AB_2088164 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | Mouse Brain Tumor Hochest (Blue) CXCL12 (Green Abcam #AB25117) mCherry (Magenta, Novus #NBP2-25158) |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 | Unconjugated | AB_2892755 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 | Unconjugated | AB_2892755 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 (Unconjugated) | AF532 | AB_2892755 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q53X90 | Primary Antibody | CXCL13 | Human | Goat | IgG | Polyclonal | R&D Systems | AF801 (Unconjugated) | AF532 | AB_2892755 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CXCL13 (blue, catalog number AF801) and Lumican (magenta, catalog number AF2846) |
| P49682 | Primary Antibody | CXCR3 | Human | Mouse | IgG1 | 49801 | R&D Systems | FAB160N | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | No | 0000-0002-6604-2065 | NA | 0000-0002-6604-2065 | Human tonsil FFPE: CXCR3 (cyan, catalog number FAB160N) |
| P49682 | Primary Antibody | CXCR3 | Human | Mouse | IgG1 | 1C6/CXCR3 | BD Biosciences | 561320 | AF700 | AB_10611719 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 (Sodium Citrate buffer) for 45 minutes | NA | No | 0000-0002-6604-2065 | NA | 0000-0002-6604-2065 | Human tonsil FFPE: CXCR3 (cyan, catalog number 561320) |
| P70658 | Primary Antibody | CXCR4 | Mouse | Rabbit | IgG | Polyclonal | Novus Biologicals | NB-100 74396 | NA | AB_1048484 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | Mouse Brain Tumor Hochest (Blue) CXCR4 (Green Novus # NB-100 74396) |
| Q9EQ16 | Primary Antibody | CXCR6 | Mouse | Rat | IgG2b | FAB2145R | Novus Biologicals | 221002 | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| P56485 | Primary Antibody | CXCR7 | Mouse | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-24779G | DL488 | NA | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | Mouse Brain Tumor Hoechst (Blue) CXCR7 (Green, catalog number NBP2-24779G) |
| NA | Primary Antibody | Cytokeratin | Mouse | Mouse | IgG1 | C-11 | BioLegend | 628604 | AF647 | AB_2563652 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: CD206 (cyan, catalog number 141717) and Cytokeratin (purple, catalog number 628604) |
| NA | Primary Antibody | Cytokeratin | Mouse | Mouse | IgG1 | C-11 | BioLegend | 628608 | AF488 | AB_2563652 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: Cytokeratin (yellow, catalog number 628608) and SIRPalpha (white, catalog number 144027) |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 50-9003-82 | eF660 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 | NA |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Novus Biologicals | NBP2-33200AF700 | AF700 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | Human tonsil FFPE: CD20 (cyan, catalog number 42-0202-82) and pan-Cytokeratin (yellow, catalog number NBP2-33200AF700) |
| NA | Primary Antibody | Cytokeratin (Pan) | Canine | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine melanoma skin FFPE: Lumican (blue, catalog number AF2846 and A-21447), a-SMA (magenta, catalog number 41-9760-82), pan-cytokeratin (green, catalog number 53-9003-80), and Hoechst (yellow, catalog number 40046) |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Novus Biologicals | NBP2-33200AF750 | AF750 | AB_2868569 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 42-9003-82 | eF615 | AB_10804663 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 41-9003-80 | eF570 | AB_11217482 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | BioLegend | 914204 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 50-9003-82 | eF660 | AB_2574301 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | Cytokeratin (Pan) | Human | Mouse | IgG1 | AE1/AE3 | Thermo Fisher Scientific | 53-9003-82 | AF488 | AB_2574301 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P13645 | Primary Antibody | Cytokeratin 10 | Human | Mouse | IgG1 | DE-K10 | Novus Biologicals | NBP2-54402AF488 | AF488 | AB_2892746 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| P02533 | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 | Unconjugated | AB_2616962 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02533 | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 (Unconjugated) | AF647 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02533 | Primary Antibody | Cytokeratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 | Unconjugated | AB_2616962 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | Human skin: Keratin 14 (yellow, catalog number 906004 and A-32932) and CD44 (purple, catalog number 103016) |
| P05784 | Primary Antibody | Cytokeratin 18 | Mouse | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-14263 | Unconjugated | AB_2133178 | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| P08729 | Primary Antibody | Cytokeratin 7 | Human | Rat | IgG2a | W16155A | BioLegend | 601606 | AF488 | AB_2728458 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3218-4479 | NA | 0000-0002-3218-4479 | Human liver: Cytokeratin 7 (green, catalog number 601606), CD44 (blue, based on catalog number 103002) and CD45 (magenta, catalog number 304060) |
| NA | Nuclear Dye | DAPI | Zebrafish | NA | NA | NA | Thermo Fisher Scientific | D1306 | NA | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | 9E9A8 | BioLegend | 330112 | AF647 | AB_1186092 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: DC-SIGN (magenta, catalog number 330112) and Vimentin (yellow, based on catalog number 677802), Human lymph node: CD3 (purple, catalog number 300446) and DC-SIGN (cyan, catalog number 330112) |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | 102E11.06 | Eurobio Scientific (Dendritics) | DDX0202A647-100 | AF647 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | 102E11.06 | Eurobio Scientific (Dendritics) | DDX0202A647-100 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2b | C209/1781 | Novus Biologicals | NBP2-54351AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | LSBio | LS-B3782 | Unconjugated | AB_10689801 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | 9E9A8 | BioLegend | 330112 | AF647 | AB_1186092 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | DC28 | R&D Systems | MAB16211-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | LSBio | LS-B3782 | Unconjugated | AB_10689801 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| O15075 | Primary Antibody | DCAMKL1 | Human | Rabbit | IgG | Polyclonal | Abcam | ab31704 | Unconjugated | AB_873537 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9JLM8 | Primary Antibody | DCAMKL1 | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab37994 | Unconjugated | AB_873538 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| O15075 | Primary Antibody | DCAMKL1 | Human | Rabbit | IgG | EPR6085 | Abcam | ab187184 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| Q16832 | Primary Antibody | DDR2 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2538 | Unconjugated | AB_416613 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE: DDR2 (green, catalog number AF2538) |
| Q60767 | Primary Antibody | DEC205 | Mouse | Rat | IgG2a | NLDC-145 | BioLegend | 138204 | AF647 | AB_2137655 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD8 (cyan, catalog number 100738), DEC205 (purple, catalog number 138204), and CD169 (yellow, catalog number 142404) |
| Q60767 | Primary Antibody | DEC205 | Mouse | Rat | IgG2a | NLDC-145 | BioLegend | 138204 | AF647 | AB_2137655 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: DEC205 (magenta, catalog number 138204) and SIRPalpha (cyan, catalog number 144024) |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Desmin (orange, catalog number ab185033) and Hoechst (blue, catalog number 40046) |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: LYVE-1 (yellow, catalog number AF2089), Desmin (cyan, catalog number ab185033), and Hoechst (blue, catalog number 40046) |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P17661 | Primary Antibody | Desmin | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab15200 | Unconjugated | AB_301744 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| P17661 | Primary Antibody | Desmin | Human | Rabbit | IgG | Y66 | Abcam | ab185033 | AF488 | AB_2892748 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P12830 | Primary Antibody | E-cadherin | Human | Rat | IgG1 | DECMA-1 | BioLegend | 147307 | AF647 | AB_2563954 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Nasal Polyp | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0004-8162-409X | NA | 0009-0004-8162-409X | Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), EpCAM (blue, catalog number 324228) and DAPI (cyan), Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), and DAPI (cyan) |
| P09803 | Primary Antibody | E-cadherin | Mouse | Rat | IgG1 | DECMA-1 | BioLegend | 147308 | AF647 | AB_2563955 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | Mouse liver: E-Cadherin (red, catalog number 147308), and CD45 (cyan, catalog number 103128), Mouse liver: E-Cadherin (blue, catalog number 147308) and MHCII (magenta, catalog number 107622) |
| P32249 | Primary Antibody | EBI2 | Human | Mouse | IgG2a | SA313E4 | BioLegend | 368903 | AF647 | AB_2566384 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P32249 | Primary Antibody | EBI2 | Human | Rabbit | IgG | Polyclonal | RayBiotech | 119-10961 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P32249 | Primary Antibody | EBI2 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NLS46 | Unconjugated | AB_10001402 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | EBOV NP | Mouse | Rabbit | NA | Polyclonal | IBT BioServices | 0301-012 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| NA | Primary Antibody | EBOV NP | Mouse | Rabbit | NA | Polyclonal | IBT BioServices | 0301-012 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| Q99JW5 | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | BioLegend | 118212 | AF647 | AB_1134101 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lung: EpCAM (blue, catalog number 118212), MHCII (magenta, catalog number 107622), and CD169 (green, catalog number 142404) |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324228 | AF594 | AB_2563209 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: CD45 (cyan, catalog number 58-0459-41) and EpCAM (blue, catalog number 324228) |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324210 | AF488 | AB_756084 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: EpCAM (blue, catalog number 324210) and CD44 (yellow, catalog number 103016), Human jejunum: EpCAM (purple, catalog number 324210) and CD49a (cyan, catalog number 328310) |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324235 | BV510 | AB_2632934 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG1 | 1B7 | Thermo Fisher Scientific | 50-9326-42 | eF660 | AB_10598658 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16422 | Primary Antibody | EpCAM | Human | Rabbit | IgG | Polyclonal | Abcam | ab71916 | Unconjugated | AB_1603782 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324202 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324228 | AF594 | AB_2563209 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Metastatic | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node metastatic: CD4 (blue, catalog number 300526) and EpCAM (red, catalog number 324228) |
| Q99JW5 | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | BioLegend | 118222 | AF594 | AB_2563322 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse gut: EpCAM (magenta, catalog number 118222), CD8 (blue, catalog number 100738), and CD31 (green, catalog number 102516), Mouse gut: EpCAM (blue, catalog number 118222), IgD (red, catalog number 405730), and CD11c (cyan, catalog number 117312), Mouse gut: EpCAM (cyan, catalog number 118222), B220 (purple, catalog number 103248), and CD117 (yellow, catalog number 105827), Mouse gut: EpCAM (blue, catalog number 118222), CD31 (magenta, catalog number 102516), and MHCII (green, catalog number 107622) |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324243 | AF700 | AB_2750488 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| Q99JW5 | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | BD Biosciences | 747748 | BV510 | AB_2872217 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324212 | AF647 | AB_756086 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | ERTR7 | Mouse | Rat | IgG2a | ER-TR7 | Novus Biologicals | NB100-64932IR | DL755 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | NA | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | NA |
| Q14246 | Primary Antibody | F4/80 | Mouse | Rat | IgG2b | F4/80 | Abcam | ab204467 | AF647 | AB_2810932 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123130 | AF700 | AB_2293450 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q14246 | Primary Antibody | F4/80 | Mouse | Rat | IgG2b | F4/80 | Abcam | ab204467 | AF647 | AB_2810932 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123122 | AF647 | AB_893480 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q3S4B0 | Primary Antibody | F4/80 | Mouse | Rabbit | IgG | D2S9R | Cell Signaling Technology | 70076 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| Q3S4B0 | Primary Antibody | F4/80 | Mouse | Rabbit | IgG | D2S9R | Cell Signaling Technology | 70076 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123122 | AF647 | AB_893480 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123132 | BV421 | AB_11203717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse spleen: MHCII (blue, catalog number 107622) and F4/80 (magenta, catalog number 123132) |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | Thermo Fisher Scientific | 12-4801-82 | PE | AB_465923 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | F4/80 | Mouse | Rat | IgG2a | BM8 | BioLegend | 123132 | BV421 | AB_11203717 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: F4/80 (cyan, catalog number 123132), CD68 (blue, catalog number 137017) and NK1.1 (magenta, catalog number 108731) |
| NA | Blocking Reagent | Fc Block | Mouse | Rat | IgG2b | 2.4G2 | BD Biosciences | 553141 | Unconjugated | AB_394656 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Blocking Reagent | Fc Block | Human | Human | IgG1 | Fc1 | BD Biosciences | 564220 | Unconjugated | AB_2869554 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q8TBJ5 | Primary Antibody | FEZF2 | Human | Rabbit | IgG | Polyclonal | Abcam | ab214186 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P02671 | Primary Antibody | Fibrinogen | Human | Sheep | IgG | Polyclonal | Abcam | ab118533 | Unconjugated | AB_10900171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02671 | Primary Antibody | Fibrinogen | Human | Sheep | IgG | Polyclonal | Abcam | ab118533 | Unconjugated | AB_10900171 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q12884 | Primary Antibody | Fibroblast activation protein alpha | Human | Rabbit | IgG | Polyclonal | MilliporeSigma | ABT11 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02751 | Primary Antibody | Fibronectin | Human | Mouse | IgG1 | 2F4 | Novus Biologicals | NBP2-22113AF532 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Fluorescein/Oregon Green | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | A-11090 | AF488 | AB_221562 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q12951 | Primary Antibody | FOXI1 | Human | Mouse | IgG1 | OTI1D4 | Novus Biologicals | NBP2-70747AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| Q9H334 | Primary Antibody | FOXP1 | Human | Mouse | IgG2a | JC12 | Novus Biologicals | NB100-65125 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 53-5773-82 | AF488 | AB_763537 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 58-5773-82 | AF532 | AB_11218870 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q9BZS2 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 17-4777-42 | APC | AB_10804651 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 12-5773-82 | PE | AB_465936 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BZS2 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 17-4777-42 | APC | AB_10804651 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 14-4777-82 | Unconjugated | AB_467556 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Rabbit | IgG | 1054C | Cell IDx | HI06B-005 | UT014 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 14-4777-82 | Unconjugated | AB_467556 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 41-5773-82 | eF570 | AB_11219073 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 13-4777-82 | Biotin | AB_466655 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 53-4777-41 | AF488 | AB_10852836 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 50-4777-42 | eF660 | AB_2574219 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human tonsil FFPE : FOXP3 (yellow, catalog number 41-4777-82) and Hoechst (blue, catalog number 40046) |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 41-4777-82 | eF570 | AB_2573609 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD25 (cyan, catalog number 356128) and FOXP3 (yellow, catalog number 41-4777-82) |
| NA | Primary Antibody | Francisella Tularensis, LPS | Mouse | Mouse | IgG2a | 1.B.288 | US Biological Life Sciences | F6070-02X | Unconjugated | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | NA | Yes | 0000-0001-9189-7172 | NA | 0000-0001-9189-7172 | NA |
| Q14392 | Primary Antibody | GARP | Human | Mouse | IgG2b | 7B11 | BioLegend | 352502 | Unconjugated | AB_10897447 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q14392 | Primary Antibody | GARP | Human | Mouse | IgG2b | Plato-1 | Enzo | ALX-804-867-C100 | Unconjugated | AB_2051806 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q14392 | Primary Antibody | GARP | Human | Mouse | IgG2b | 7B11 | BioLegend | 352503 | PE | AB_10896792 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P23771 | Primary Antibody | GATA-3 | Human | Mouse | IgG1 | L50-823 | BD Biosciences | 560077 | AF488 | AB_1645303 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P14136 | Primary Antibody | GFAP | Mouse | Mouse | IgG2b | SMI 25 | BioLegend | 837504 | AF647 | AB_2632641 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | NA |
| P03995 | Primary Antibody | GFAP | Mouse | Mouse | IgG1 | GA5 | Thermo Fisher Scientific | 41-9892-82 | eF570 | AB_2573656 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | Mouse Brain Tumor Hochest (Cyan) GFAP (Orange Thermo #41-9892-82) |
| Q58EE9 | Primary Antibody | GFAP | Zebrafish | Mouse | IgG2b | SMI 25 | BioLegend | 837508 | AF488 | AB_2728555 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P42212 | Primary Antibody | GFP | Jellyfish GFP transgene | Chicken | IgY | Monoclonal | Thermo Fisher Scientific | A10262 | Unconjugated | AB_2534023 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q76IQ4 | Primary Antibody | Ghrelin | Human | Mouse | IgG2b | 891240 | R&D Systems | MAB8149 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | GL-7 | Mouse | Rat | IgM | GL7 | BD Biosciences | 561530 | PE | AB_10715834 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | GL7 | Mouse | Rat | IgM | GL7 | BD Biosciences | 561530 | PE | AB_10715834 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q7ZVF2 | Primary Antibody | Glutamine synthetase | Zebrafish | Mouse | IgG1 | 1B6G4 | Proteintech | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P15104 | Primary Antibody | Glutamine synthetase | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab49873 | Unconjugated | AB_880241 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| P15104 | Primary Antibody | Glutamine synthetase | Mouse | Mouse | IgG1 | 1B6G4 | Proteintech | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Glutamine synthetase (green, catalog number CL488-66323) and Hoechst (blue, catalog number 40046) |
| A0A8C6NQP0 | Primary Antibody | Glutamine synthetase | Turquoise Killifish | Mouse | IgG1 | 1B6G4 | Proteintech | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Rabbit | IgG | Polyclonal | Abcam | ab49873 | Unconjugated | AB_880241 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| P51121 | Primary Antibody | Glutamine synthetase | African Clawed Frog (Xenopus laevis) | Mouse | IgG1 | 1B6G4 | Proteintech | CL488-66323 | CoraLite Plus AF488 | AB_2883309 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2bc | GLUL/6600 | Novus Biologicals | NBP3-14023AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Rabbit | IgG | EPR13022(B) | Abcam | ab302584 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE: Glutamine synthetase (cyan, catalog number Ab302584) |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2a | OTI1F4 | Novus Biologicals | NBP2-70834AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE: Glutamine synthetase (magenta, catalog number NBP2-70834AF647) |
| P15104 | Primary Antibody | Glutamine synthetase | Human | Mouse | IgG2a | OTI1F4 | Novus Biologicals | NBP2-70834AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02724 | Primary Antibody | Glycophorin A | Human | Rabbit | IgG | EPR8200 | Abcam | ab218372 (Unconjugated) | AF555 | AB_2892758 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | Human spleen: HLA-DR (magenta, catalog number 1032136) and Glycophorin A (cyan, based on catalog number ab218372) |
| P02724 | Primary Antibody | Glycophorin A | Human | Rabbit | IgG | EPR8200 | Abcam | ab218372 (Unconjugated) | AF555 | AB_2892758 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q90WX5 | Primary Antibody | GNAT2 | Zebrafish | Rabbit | IgG | Polyclonal | MBL International Corporation | PM075 | Unconjugated | AB_2864374 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Primary Antibody | Go-alpha | Zebrafish | Mouse | IgG1 | 2A | MilliporeSigma | MAB3073 | Unconjugated | AB_94671 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Canine | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21447 | AF647 | AB_2535864 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine melanoma skin FFPE: Lumican (blue, catalog number AF2846 and A-21447), a-SMA (magenta, catalog number 41-9760-82), pan-cytokeratin (green, catalog number 53-9003-80), and Hoechst (yellow, catalog number 40046) |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10091 | DL755 | AB_2556671 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11055 | AF488 | AB_2534102 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21084 | AF680 | AB_2535741 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21084 | AF680 | AB_2535741 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A32816 | AF555 (Plus) | AB_2762839 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Iba1 (cyan, catalog number ab5076 and A32816) and Hoechst (blue, catalog number 40046) |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21447 | AF647 | AB_2535864 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-32930 | AF800 (Plus) | AB_2762842 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10091 | DL755 | AB_2556671 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11056 | AF546 | AB_2534103 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11058 | AF594 | AB_2534105 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Abcam | ab175745 | AF750 | AB_2924800 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11055 | AF488 | AB_2534102 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21432 | AF555 | AB_2535853 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21084 | AF680 | AB_2535741 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| NA | Secondary Antibody | Goat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11055 | AF488 | AB_2534102 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q62011 | Primary Antibody | gp38 | Mouse | Syrian Hamster | IgG | 8.1.1 | BioLegend | 127401 | Unconjugated | AB_1089186 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: gp38 (purple, catalog number 127401) and SIRPalpha (cyan, catalog number 144024) |
| F7CLT4 | Primary Antibody | Granzyme B | Rhesus Macaque | Mouse | IgG2a | GrB-7 | Agilent | M7235 | Unconjugated | AB_2114697 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P10144 | Primary Antibody | Granzyme B | Human | Rabbit | IgG | EP230 | Cell Marque | 262R-17 | Unconjugated | AB_2889344 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P10144 | Primary Antibody | Granzyme B | Human | Rabbit | IgG | Polyclonal | Abcam | ab4059 | Unconjugated | AB_304251 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | Granzyme B | Canine | Mouse | IgG1 | GB12 | Thermo Fisher Scientific | MHGB04 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | NA |
| NA | Secondary Antibody | Hamster IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21451 | AF647 | AB_2535868 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q64525 | Primary Antibody | Histone H2B | Mouse | Rat | IgG2a | W17025A | BioLegend | 606301 | Unconjugated | AB_2728465 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| P02302 | Primary Antibody | Histone H3 | Mouse | Mouse | IgG3 | 1B1-B2 | BioLegend | 819406 | AF594 | AB_2715803 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| P68431 | Primary Antibody | Histone H3 (citrulline R2 + R8 + R17) | Human | Rabbit | IgG | Polyclonal | Abcam | ab5103 | Unconjugated | AB_304752 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670 | PE | NA | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: HLA-DR (green, catalog number 307620), Human lymph node: HLA-DR (purple, catalog number 307620) and Valpha7.2 (yellow, catalog number 351726) |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307602 (Unconjugated) | AF532 | NA | Custom | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307619 | AF488 | AB_493176 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: HLA-DR (cyan, catalog number 307620) and CD69 (red, catalog number 310918) |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307622 | AF647 | AB_493177 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307625 | AF700 | AB_493770 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human tonsil FFPE: HLA-DR (magenta, catalog number NBP2-47670AF647) |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670AF532 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Abcam | ab176408 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Abcam | ab20181 | AF647 | AB_445401 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| NA | Primary Antibody | HLA-DR | Canine | Mouse | IgG1 | TAL 1B5 | Abcam | ab176408 (Unconjugated) | iF594 | NA | Custom | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine lymph node FFPE: LYVE1 (magenta, catalog number AF2089 and A21203) and HLA-DR (blue, catalog number ab176408) |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Abcam | ab176408 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670PE | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307620 | AF488 | AB_493175 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human liver: HLA-DR (cyan, catalog number 307620) and Vimentin (red, catalog number 677807) |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG1 | TAL 1B5 | Novus Biologicals | NB600-989AF532-0.1ml | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | Human tonsil FFPE: HLA-DR (blue, catalog number NB600-989AF532-0.1ml) and pan-Cytokeratin (yellow, catalog number NBP2-33200AF700) |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | BioLegend | 307622 | AF647 | AB_493177 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: HLA-DR (pink, catalog number 307622) and EpCAM (blue, catalog number 324228) |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670 | PE | NA | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Caprico Biotechnologies | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: HLA-DR (magenta, catalog number 1032136) and Glycophorin A (cyan, based on catalog number ab218372) |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human FFPE follicular lymphoma lymph node: HLA-DR (red, catalog number NBP2-47670AF488), Human FFPE follicular lymphoma lymph node: HLA-DR (red, catalog number NBP2-47670AF488) and PD-1 (cyan, catalog number ab275126) |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | LN-3 | Novus Biologicals | NBP2-47670AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Caprico Biotechnologies | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Caprico Biotechnologies | 1032136 | iF594 | AB_2892750 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2a | L243 | Novus Biologicals | NB100-77855AF750 | AF750 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01903 | Primary Antibody | HLA-DR | Human | Mouse | IgG2b | LN-3 | Novus Biologicals | NBP2-47670AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01911 | Primary Antibody | HLA-DRB | Human | Mouse | IgG2b | SPM288 | Novus Biologicals | NBP2-45312-0.02mg | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P63158 | Primary Antibody | HMGB-1 | Mouse | Mouse | IgG2 | 3E8 | BioLegend | 651406 | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: Hoechst (blue, catalog number 40046), CD106 (cyan, catalog number 305806), and CD31 (red, catalog number 303106) |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human kidney: Hoechst (blue, catalog number 40046) and Vimentin (magenta, catalog number 677807) |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: Hoechst (blue, catalog number 40046) and Collagen IV (red, catalog number ab6586) |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| NA | Nuclear Dye | Hoechst 33342 | Human | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Nuclear Dye | Hoechst 33342 | Rhesus Macaque | NA | NA | NA | Biotium | 40046 | NA | NA | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 | NHP spleen: Hoechst (red, catalog number 40046) and CD49a (cyan, catalog number ab243032 and A-31570) |
| NA | Primary Antibody | HuC/D | Turquoise Killifish | Mouse | IgG2b | 16A11 | Thermo Fisher Scientific | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Primary Antibody | HuC/D | African Clawed Frog (Xenopus laevis) | Mouse | IgG2b | 16A11 | Thermo Fisher Scientific | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Primary Antibody | HuC/D | Zebrafish | Mouse | IgG2b | 16A11 | Thermo Fisher Scientific | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q14576 | Primary Antibody | HuC/HuD neuronal protein | Human | Mouse | IgG2b | 16A11 | Thermo Fisher Scientific | A21271 | Unconjugated | AB_221448 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: HuC/HuD neuronal protein (magenta, catalog number A21271) and Hoechst (blue, catalog number 40046) |
| Q9EQX4 | Primary Antibody | Iba-1 | Mouse | Rabbit | IgG | Polyclonal | FUJIFILM Wako Chemicals U.S.A. Corporation | 019-19741 | NA | AB_839504 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | Mouse Brain Tumor Hochest (Cyan) Iba (Green_Fuji #019-19741) |
| P55008 | Primary Antibody | Iba1 | Human | Goat | IgG | Polyclonal | Abcam | ab5076 | Unconjugated | AB_2224402 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Iba1 (cyan, catalog number ab5076 and A32816) and Hoechst (blue, catalog number 40046) |
| Q9Y6W8 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313514 | AF488 | AB_2122584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9Y6W8 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313514 | AF488 | AB_2122584 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: ICOS (cyan, catalog number 313514) and PD-1 (magenta, catalog number 329906), Human lymph node: ICOS (yellow, catalog number 313514) and CD35 (purple, catalog number 333406) |
| Q9Y6W8 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313527 | AF700 | AB_2566125 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9Y6W8 | Primary Antibody | ICOS | Human | Rabbit | IgG | SP98 | Abcam | ab105227 | Unconjugated | AB_10710236 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9Y6W8 | Primary Antibody | ICOS | Human | Armenian Hamster | IgG | CS98.4A | BioLegend | 313515 | AF647 | AB_2122583 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01878 | Primary Antibody | IgA | Mouse | Goat | IgG | Polyclonal | SouthernBiotech | 1040-32 | AF555 | AB_2794378 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01878 | Primary Antibody | IgA | Mouse | Goat | IgG | Polyclonal | SouthernBiotech | 1040-32 | AF555 | AB_2794378 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01876 | Primary Antibody | IgA1 | Human | Mouse | IgG1 | B3506B4 | SouthernBiotech | 9130-31 | AF647 | AB_2796658 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01876 | Primary Antibody | IgA1 | Human | Mouse | IgG1 | B3506B4 | SouthernBiotech | 9130-31 | AF647 | AB_2796658 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01877 | Primary Antibody | IgA2 | Human | Mouse | IgG1 | A9604D2 | SouthernBiotech | 9140-30 | AF488 | AB_2796665 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01877 | Primary Antibody | IgA2 | Human | Mouse | IgG1 | A9604D2 | SouthernBiotech | 9140-30 | AF488 | AB_2796665 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01880 | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | BioLegend | 348229 | AF700 | AB_2563334 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405718 | AF488 | AB_10730619 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse gut: EpCAM (blue, catalog number 118222), IgD (red, catalog number 405730), and CD11c (cyan, catalog number 117312) |
| P01880 | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | BioLegend | 348216 | AF488 | AB_11150595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: IgD (magenta, catalog number 348216), Human lymph node: IgD (blue, catalog number 348216) and PD-1 (green, catalog number 329906) |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405730 | AF700 | AB_2563341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01880 | Primary Antibody | IgD | Human | Mouse | IgG2a | IA6-2 | BioLegend | 348216 | AF488 | AB_11150595 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405718 | AF488 | AB_10730619 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405740 | AF594 | AB_2565572 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P01881 | Primary Antibody | IgD | Mouse | Rat | IgG2a | 11-26c.2a | BioLegend | 405723 | BV510 | AB_2562742 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | Mouse lymph node: IgD (green, catalog number 405723) and B220 (blue, catalog number 103226) |
| P06336 | Primary Antibody | IgE | Mouse | Rat | IgG1 | RME-1 | BioLegend | 406908 | PE | AB_493290 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | NA |
| P01868 | Primary Antibody | IgG1 | Mouse | Rat | IgG | RMG1-1 | BioLegend | 406616 | BV421 | AB_2562234 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | NA |
| P07476 | Primary Antibody | Involucrin | Human | Rabbit | IgG | Polyclonal | Abcam | ab53112 | Unconjugated | AB_880813 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P07476 | Primary Antibody | Involucrin | Human | Mouse | IgG1 | SY5 | Santa Cruz Biotechnology | sc-21748 AF488 | AF488 | AB_2128119 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| Q15306 | Primary Antibody | IRF4 | Human | Rabbit | IgG | EP5699 | Abcam | ab252024 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15306 | Primary Antibody | IRF4 | Human | Sheep | IgG | Polyclonal | R&D Systems | AF5525 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15306 | Primary Antibody | IRF4 | Human | Mouse | IgG1 | MUM1p | Novus Biologicals | NB200-356 | Unconjugated | AB_10003502 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15306 | Primary Antibody | IRF4 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP1-82814 | Unconjugated | AB_11038596 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15306 | Primary Antibody | IRF4 | Human | Rat | IgG1 | IRF4.3E4 | BioLegend | 646404 | PE | AB_2563005 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q64287 | Primary Antibody | IRF4 | Mouse | Rat | IgG1 | 3.E.4 | Thermo Fisher Scientific | 11-9858-82 | FITC | AB_2572538 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15306 | Primary Antibody | IRF4 | Human | Mouse | IgG1 | MUM1p | Novus Biologicals | NB200-356 | Unconjugated | AB_10003502 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15306 | Primary Antibody | IRF4 | Human | Rat | IgG1 | IRF4.3E4 | BioLegend | 646404 | PE | AB_2563005 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD138 (cyan, catalog number 356504) and IRF4 (red, catalog number 646404) |
| Q15306 | Primary Antibody | IRF4 | Human | Mouse | IgG1 | 4G10 | Novus Biologicals | NBP1-47396 | Unconjugated | AB_10010349 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q02556 | Primary Antibody | IRF8 | Human | Rabbit | IgG | E6J8Q | Cell Signaling Technology | 80174S | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| NA | Lectin | Isolectin GS-IB4 | Human | NA | NA | NA | Thermo Fisher Scientific | I21412 | AF568 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | NA |
| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | Thermo Fisher Scientific | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | Thermo Fisher Scientific | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | Thermo Fisher Scientific | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | Thermo Fisher Scientific | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Nuclear Dye | JOJO-1 | Mouse | NA | NA | NA | Thermo Fisher Scientific | J11372 | NA | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02533 | Primary Antibody | Keratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 (Unconjugated) | AF647 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P13647 | Primary Antibody | Keratin 5 | Human | Rabbit | IgG | Polyclonal | BioLegend | 905504 (Unconjugated) | AF532 | AB_3095835 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human thymus: TCRgammadelta (magenta, catalog number 331210) and Keratin 5 (green, catalog number based on 905504) |
| E9PVX7 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 558616 | AF488 | AB_647087 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 | Mouse lymph node: Ki67 (grey, catalog number 558616) |
| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| E9PVX9 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD169 (green, catalog number 142404) and Ki-67 (magenta, catalog number 561277) |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB500-170b | Biotin | AB_1660247 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| E9PVX8 | Primary Antibody | Ki-67 | Mouse | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB500-170b | Biotin | AB_1660247 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P79192 | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | BD Biosciences | 563462 | BV510 | AB_2738221 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P79192 | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | BD Biosciences | 562899 | BV421 | AB_2686897 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| P79192 | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | BD Biosciences | 558616 | AF488 | AB_647087 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Rat | IgG2a | SolA15 | Thermo Fisher Scientific | 606-5698-82 | AF660 | AB_2896286 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | Mouse Brain Tumor Hochest (Blue) Iba (Cyan, Fuji #019-19741) mCherry (Red, Novus #NBP2-25158) Ki67 (Magenta Thermo #606-5698-82) |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB500-170b | Biotin | AB_1660247 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P79192 | Primary Antibody | Ki-67 | Rhesus Macaque | Mouse | IgG1 | B56 | BD Biosciences | 558615 | AF647 | AB_647130 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB500-170 | Unconjugated | AB_10001977 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: Ki-67 (yellow, catalog number 561277) and CD21 (cyan, based on catalog number 354902) |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| E9PVX6 | Primary Antibody | Ki-67 | Mouse | Rat | IgG2a | SolA15 | Thermo Fisher Scientific | 48-5698-80 | eF450 | AB_11151155 | Stock | Ce3D-IBEX | 4% PFA Fixed Agarose | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 1 hour | Yes | 0000-0002-0835-911X | NA | 0000-0002-0835-911X | NA |
| Q9BYG3 | Primary Antibody | Ki-67 | Human | Mouse | IgG1 | B56 | BD Biosciences | 561277 | AF700 | AB_10611571 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD23 (magenta, based on catalog number 338502) and Ki-67 (green, catalog number 561277) |
| O88713 | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | BD Biosciences | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| O88713 | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | BD Biosciences | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| O88713 | Primary Antibody | KLRG1 | Mouse | Syrian Hamster | IgG2 | 2F1 | BD Biosciences | 561619 | AF488 | AB_10898017 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18627 | Primary Antibody | LAG-3 | Human | Mouse | IgG1 | 17B4 | Novus Biologicals | NBP1-97657 | AF488 | AB_11162489 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18627 | Primary Antibody | LAG-3 | Human | Rabbit | IgG | Polyclonal | LSBio | LS-CS165666 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P18627 | Primary Antibody | LAG-3 | Human | Mouse | IgG1 | 17B4 | Novus Biologicals | NBP1-97657IR | DL755 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02545 | Primary Antibody | Lamin A | Human | Mouse | IgG3 | 133A2 | Thermo Fisher Scientific | MUB1101L1 | FITC | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Colon | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P11047 | Primary Antibody | Laminin | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB300-144AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P02468, P19137 | Primary Antibody | Laminin 1 + 2 | Mouse | Rabbit | IgG | Polyclonal | Abcam | ab7463 | Unconjugated | AB_305933 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | NA | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| Q9UJ71 | Primary Antibody | Langerin | Human | Rat | IgG2a | 929F3.01 | Novus Biologicals | DDX0362P-100 | Unconjugated | AB_2892751 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| P01137 | Primary Antibody | LAP (TGFB) | Human | Mouse | IgG1 | 27232 | R&D Systems | FAB2463G | AF488 | AB_10718684 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P59901 | Primary Antibody | LILRA4 (CD85g) | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-15012 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| D0PNI2 | Primary Antibody | LOX | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NB100-2530AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Tumor | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q12912 | Primary Antibody | LRMP | Human | Rabbit | IgG | EPR17852 | Abcam | ab202418 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 (Unconjugated) | iF594 | AB_2892764 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 (Unconjugated) | AF680 | AB_2892760 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD20 (purple, catalog number 53-0202-82) and Lumican (cyan, based on catalog number AF2846) |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| A0A8C0SV20 | Primary Antibody | Lumican | Canine | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | NA |
| A0A8C0SV20 | Primary Antibody | Lumican | Canine | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine lymph node FFPE: Lumican (magenta, catalog number AF2846 and A21447) |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 | Unconjugated | AB_2139484 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE : Lumican (cyan, catalog number AF2846 and A-21084) |
| P51884 | Primary Antibody | Lumican | Human | Goat | IgG | Polyclonal | R&D Systems | AF2846 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CXCL13 (blue, catalog number AF801) and Lumican (magenta, catalog number AF2846) |
| P51884 | Primary Antibody | Lumican | Human | Rabbit | IgG | EPR8898(2) | Abcam | ab252012 | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE : Lumican (cyan, AF2846) and Lumican PE (red, ab252012) pH 6 and 9, Human follicular lymphoma FFPE : Lumican PE (red, ab252012 ) pH 6 and 9 |
| Q8R2S8 | Primary Antibody | Ly-6G | Mouse | Rat | IgG2a | 1A8 | BioLegend | 127626 | AF488 | AB_2561340 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lung: Ly-6G (yellow, catalog number 127626), CD31 (blue, catalog number 102520), and CD8 (red, catalog number 100724) |
| Q6P698 | Primary Antibody | Lymphocyte cytosolic protein 1 (L-plastin/LCP1) | Zebrafish | Rabbit | IgG | Polyclonal | GeneTex | GTX124420 | Unconjugated | AB_11167454 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q6P698 | Primary Antibody | Lymphocyte cytosolic protein 1 (L-plastin/LCP1) | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 13025-1-AP | Unconjugated | AB_2136871 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P61626 | Primary Antibody | Lysozyme | Human | Rabbit | IgG | Polyclonal | Abcam | ab2408 | Unconjugated | AB_303050 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q8BHC0 | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | Thermo Fisher Scientific | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| F7AVS7 | Primary Antibody | LYVE-1 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q8BHC0 | Primary Antibody | LYVE-1 | Mouse | Rabbit | IgG | E3L3V | Cell Signaling Technology | 67538 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| A0A8P0NA08 | Primary Antibody | LYVE-1 | Canine | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine lymph node FFPE: LYVE1 (magenta, catalog number AF2089 and A21203) and HLA-DR (blue, catalog number ab176408) |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 (Unconjugated) | iF594 | NA | Custom | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 (Unconjugated) | AF532 | AB_2892756 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q8BHC0 | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | Thermo Fisher Scientific | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE: LYVE-1 (yellow, catalog number AF2089) and Hoechst (blue, catalog number 40046) |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 (Unconjugated) | AF532 | AB_2892756 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: LYVE-1 (yellow, catalog number AF2089), Desmin (cyan, catalog number ab185033), and Hoechst (blue, catalog number 40046) |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q8BHC0 | Primary Antibody | LYVE-1 | Mouse | Rat | IgG1 | ALY7 | Thermo Fisher Scientific | 41-0443-82 | eF570 | AB_2573596 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9UEW3 | Primary Antibody | MARCO | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-64134 | Unconjugated | AB_2643767 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | mCherrry | Mouse | Chicken | IgY | Polyclonal | Novus Biologicals | NBP2-25158 | NA | AB_2636881 | Stock | Multiplexed 2D Imaging | 4% PFA Fixed Frozen | Brain | Tumor | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-6944-6997 | NA | 0000-0002-6944-6997 | Mouse Brain Tumor Hochest (Cyan) mcherry (red Novus #NBP2-25158) |
| A0A8C0S4Q3 | Primary Antibody | Melan-A | Canine | Mouse | IgG1 | rMLANA | Thermo Fisher Scientific | 2315-MSM12-P1 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine melanoma skin FFPE: Melan-A (red, catalog number 2315-MSM12-P1 and A31570) and Hoechst (cyan, catalog number 40046) |
| Q13421 | Primary Antibody | Mesothelin | Human | Mouse | IgG2a | MB-G10 | Novus Biologicals | NB110-85538 | Unconjugated | AB_1199375 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Peritoneum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q13421 | Primary Antibody | Mesothelin | Human | Rabbit | IgG | YP158 | Novus Biologicals | NBP2-75255 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Peritoneum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107618 | AF647 | AB_493525 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse spleen: MHCII (blue, catalog number 107622) and F4/80 (magenta, catalog number 123132) |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: CD68 (cyan, catalog number 137017) and MHCII (red, catalog number 107622) |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lung: EpCAM (blue, catalog number 118212), MHCII (magenta, catalog number 107622), and CD169 (green, catalog number 142404) |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107620 | Pacific Blue | AB_493527 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107631 | BV421 | AB_10900075 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD3 (cyan, catalog number 100240) and MHCII (blue, catalog number 107631) |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | Thermo Fisher Scientific | 48-5321-82 | eF450 | AB_1272204 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107618 | AF647 | AB_493525 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | NA |
| NA | Primary Antibody | MHC-II | Mouse | Rat | IgG2b | M5/114.15.2 | BioLegend | 107622 | AF700 | AB_493727 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | Mouse liver: E-Cadherin (blue, catalog number 147308) and MHCII (magenta, catalog number 107622) |
| NA | Secondary Antibody | Mouse IgG (H+L) | Rhesus Macaque | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31570 | AF555 | AB_2536180 | Stock | Multiplexed 2D Imaging | FFPE | Spleen | NA | 0.3% Triton-X-100 | pH 6 for 20 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0009-0004-2746-8743 | NA | 0009-0004-2746-8743 | NHP spleen: CD20 (magenta, catalog number 50-0202-82) and CD49a (cyan, catalog number ab243032 and A-31570) |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | PA1-28626 | Texas Red | AB_10983944 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21235 | AF647 | AB_2535804 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11030 | AF546 | AB_2737024 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11002 | AF532 | AB_2534070 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10175 | DL755 | AB_2556755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21036 | AF700 | AB_2535707 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21236 | AF647 | AB_2535805 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21052 | AF633 | AB_2535719 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16548 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11030 | AF546 | AB_2534089 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Canine | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21203 | AF594 | AB_141633 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31570 | AF555 | AB_2536180 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11031 | AF568 | AB_144696 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21203 | AF594 | AB_141633 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21202 | AF488 | AB_141607 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-10038 | AF680 | AB_2534014 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG1 | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21121 | AF488 | AB_2535764 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG1 | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21121 | AF488 | AB_2535764 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG1 | Human | Alpaca | IgG1 | CTK0103/CTK0104 | Thermo Fisher Scientific | SA5-10331 | AF568 | AB_2868378 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Zenon Labeling Kit | Mouse IgG1 | Human | NA | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25011 | AF700 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG2a | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21137 | AF555 | AB_2535776 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG2a | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21137 | AF555 | AB_2535776 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgG2b | Human | Alpaca | IgG2b | CTK0105/CTK0106 | Thermo Fisher Scientific | SA5-10339 | AF647 | AB_2868386 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-545-020 | AF488 | AB_2340844 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-545-020 | AF488 | AB_2340844 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-605-020 | AF647 | AB_2340860 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-605-020 | AF647 | AB_2340860 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-095-140 | FITC | AB_2340791 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 30 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Mouse IgM | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 715-545-020 | AF488 | AB_2340844 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD15 (cyan, catalog number 347420 and 715-545-020) and Vimentin (red, based on catalog number 677802) |
| NA | Primary Antibody | Mycobacterium tuberculosis | Human | Rabbit | IgG | Polyclonal | Abcam | ab905 | Unconjugated | AB_306965 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 | NA |
| NA | Primary Antibody | Mycobacterium tuberculosis Ag85B | Human | Rabbit | IgG | Polyclonal | Abcam | ab43019 | Unconjugated | AB_776575 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 | NA |
| NA | FlexAble CoraLite Plus 750 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | Proteintech | KFA004 | CoraLite Plus AF750 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | FlexAble CoraLite Plus 555 Antibody Labeling Kit for Mouse IgG1 | NA | Zebrafish | NA | NA | NA | Proteintech | KFA022 | CoraLite Plus AF555 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | FlexAble CoraLite Plus 647 Antibody Labeling Kit for Mouse IgG1 | NA | Zebrafish | NA | NA | NA | Proteintech | KFA023 | CoraLite Plus AF647 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | FlexAble CoraLite Plus 488 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | Proteintech | KFA001 | CoraLite Plus AF488 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | FlexAble CoraLite Plus 550 Antibody Labeling Kit for Rabbit IgG | NA | Zebrafish | NA | NA | NA | Proteintech | KFA002 | CoraLite Plus AF550 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | Thermo Fisher Scientific | S21384 | AF750 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Avidin/Biotin Blocking Kit | NA | Human | NA | NA | NA | Abcam | ab64212 | NA | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | Thermo Fisher Scientific | 48-4317-82 | eF450 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Avidin/Biotin Blocking Kit | NA | Human | NA | NA | NA | Abcam | ab64212 | NA | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | Thermo Fisher Scientific | S11224 | AF532 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | AAT Bioquest | 16956 | iF514 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | Jackson ImmunoResearch | 016-620-084 | AF680 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | BioLegend | 405203 | PE | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | Thermo Fisher Scientific | S21384 | AF750 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Streptavidin Conjugate | NA | Human | NA | NA | NA | BioLegend | 405235 | AF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q13562 | Primary Antibody | NeuroD1 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP2-98697AF647 | AF647 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P08246 | Primary Antibody | Neutrophil elastase | Human | Sheep | IgG | Polyclonal | LSBio | LS-B4244-50 | Unconjugated | AB_10912151 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| P12036 | Primary Antibody | NF-H | Human | Mouse | IgG1 | SMI32 | BioLegend | 801705 | AF488 | AB_2715876 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | NF-H/NF-M | Human | Mouse | IgG1 | SMI310 | BioLegend | 837711 | AF488 | AB_2750293 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P07196 | Primary Antibody | NF-L | Human | Mouse | IgG1 | NFL3 | BioLegend | 845907 | AF647 | AB_2728564 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P30548 | Primary Antibody | NK1.1 | Mouse | Mouse | IgG2a | PK136 | BioLegend | 108731 | BV421 | AB_10895916 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| P30548 | Primary Antibody | NK1.1 | Mouse | Mouse | IgG2a | PK136 | BioLegend | 108731 | BV421 | AB_10895916 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q8C567 | Primary Antibody | NKp46 | Mouse | Rat | IgG2a | 29A1.4 | Thermo Fisher Scientific | 12-3351-82 | PE | AB_1210743 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Prostate | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| Q92823 | Primary Antibody | NrCAM | Human | Mouse | IgG2a | N343/26 | BioLegend | 821602 | Unconjugated | AB_2734588 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| NA | Primary Antibody | Nuclear Pore Complex | Mouse | Mouse | IgG1 | MAb414 | BioLegend | 682202 | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | NA |
| P22736 | Primary Antibody | Nur77 | Human | Rabbit | IgG | Polyclonal | Abcam | ab48789 | Unconjugated | AB_881709 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P22736 | Primary Antibody | Nur77 | Human | Mouse | IgG2b | 1E10A15 | BioLegend | 653504 | AF594 | AB_2563321 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P04000 | Primary Antibody | Opsin Red/Green | Mouse | Rabbit | IgG | Polyclonal | MilliporeSigma | AB5405 | Unconjugated | AB_177456 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | NA |
| P04000 | Primary Antibody | Opsin Red/Green | Human | Rabbit | IgG | Polyclonal | MilliporeSigma | AB5405 | Unconjugated | AB_177456 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Opsin Red (red, catalog number AB5405) and Hoechst (blue, catalog number 40046) |
| Q9W6A7/Q9W6A5 | Primary Antibody | Opsin Red/Green | Zebrafish | Rabbit | IgG | Polyclonal | MilliporeSigma | AB5405 | Unconjugated | AB_177456 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P15863 | Primary Antibody | PAX1 | Human | Rabbit | IgG | Polyclonal | Abcam | ab203065 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| Q02650 | Primary Antibody | Pax5 | Mouse | Rat | IgG2a | 1H9 | BioLegend | 649704 | AF647 | AB_2562425 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967, 0000-0002-1461-0999 | NA | 0000-0003-4379-8967 | Mouse lymph node: CD21 (purple, catalog number 123413) and Pax5 (cyan, catalog number 649704), Mouse lymph node: Pax5 (magenta, catalog number 649704) and CD3 (cyan, catalog number 100210) |
| P26630 | Primary Antibody | PAX6 | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 12323-1-AP | Unconjugated | AB_2159695 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| A0A8C6NQH8 | Primary Antibody | PAX6 | Turquoise Killifish | Rabbit | IgG | Polyclonal | Proteintech | 12323-1-AP | Unconjugated | AB_2159695 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P55864 | Primary Antibody | PAX6 | African Clawed Frog (Xenopus laevis) | Rabbit | IgG | Polyclonal | Proteintech | 12323-1-AP | Unconjugated | AB_2159695 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q9PTP1 | Primary Antibody | PCNA | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Primary Antibody | PCNA | Turquoise Killifish | Rabbit | IgG | Polyclonal | Proteintech | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P18248 | Primary Antibody | PCNA | African Clawed Frog (Xenopus laevis) | Rabbit | IgG | Polyclonal | Proteintech | 24036-1-AP | Unconjugated | AB_2879414 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Primary Antibody | PCNA | Turquoise Killifish | Mouse | IgG2a | PC10 | Santa Cruz Biotechnology | sc-56 | Unconjugated | AB_628110 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P18248 | Primary Antibody | PCNA | African Clawed Frog (Xenopus laevis) | Mouse | IgG2a | PC10 | Santa Cruz Biotechnology | sc-56 | Unconjugated | AB_628110 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q9PTP1 | Primary Antibody | PCNA | Zebrafish | Mouse | IgG2a | PC10 | Santa Cruz Biotechnology | sc-56 | Unconjugated | AB_628110 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P48539 | Primary Antibody | PCP4 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP1-80929 | Unconjugated | AB_11026568 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | IBEX2D Manual | FFPE | Liver | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329906 | PE | AB_940483 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329906 | PE | AB_940483 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: IgD (blue, catalog number 348216) and PD-1 (green, catalog number 329906), Human lymph node: ICOS (cyan, catalog number 313514) and PD-1 (magenta, catalog number 329906) |
| Q15116 | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329910 | AF647 | AB_940471 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329936 | AF488 | AB_2563594 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329920 | BV421 | AB_10960742 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | Abcam | ab275126 | AF555 | AB_2728811 | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human FFPE follicular lymphoma lymph node: PD-1 (cyan, catalog number ab275126), Human FFPE follicular lymphoma lymph node: HLA-DR (red, catalog number NBP2-47670AF488) and PD-1 (cyan, catalog number ab275126), Human FFPE follicular lymphoma lymph node: CTLA-4 (yellow, catalog number ab270127 and PD-1 (cyan, catalog number ab275126) |
| Q15116 | Primary Antibody | PD-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| NA | Primary Antibody | PD-1 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | BAF1086 | Biotin | AB_355833 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| NA | Primary Antibody | PD-1 | Rhesus Macaque | Goat | IgG | Polyclonal | R&D Systems | FAB7115G | AF488 | AB_10973668 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Infected | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | Abcam | ab275126 | AF555 | AB_2728811 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| A0A024FCJ9 | Primary Antibody | PD-1 | Canine | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329906 | PE | AB_940483 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q02242 | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | BioLegend | 135218 | BV421 | AB_2561447 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967, 0000-0003-1118-7432 | NA | 0000-0003-4379-8967 | Mouse lymph node: PD-1 (green, catalog number 135218), CD23 (magenta, catalog number 101611), and IgD (blue, catalog number 405718) |
| Q02242 | Primary Antibody | PD-1 | Mouse | Rat | IgG2a | 29F.1.A12 | BioLegend | 135206 | PE | AB_1877231 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Mouse | IgG1 | EH12.2H7 | BioLegend | 329906 | PE | AB_940483 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15116 | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | Abcam | ab275126 | AF555 | AB_2728811 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0009-0006-9784-2694 | NA | 0009-0006-9784-2694 | Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and PD-1 (green, Abcam cat. no. ab275126) |
| Q66HW7 | Primary Antibody | Peripherin 2 | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 18109-1-AP | Unconjugated | AB_10665364 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| O42583 | Primary Antibody | Peripherin 2 | African Clawed Frog (Xenopus laevis) | Rabbit | IgG | Polyclonal | Proteintech | 18109-1-AP | Unconjugated | AB_10665364 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| A0A8C6KR86 | Primary Antibody | Peripherin 2 | Turquoise Killifish | Rabbit | IgG | Polyclonal | Proteintech | 18109-1-AP | Unconjugated | AB_10665364 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P60709 | Cell Structure Reagent | Phalloidin | Human | NA | NA | NA | AAT Bioquest | 23122 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Phalloidin (passion pink, catalog number 23122) and Hoechst (blue, catalog number 40046) |
| P19838 | Primary Antibody | Phospho NF-Kappa B p105 | Human | Rabbit | IgG | 178F3 | Cell Signaling Technology | PA5-79525 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE: Phospho NF-Kappa B p105 (red, catalog number 4808) |
| P31751 | Primary Antibody | Phospho-Akt | Human | Rabbit | IgG | D9E | Cell Signaling Technology | 4060T | Unconjugated | AB_2315049 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE: Phospho-Akt (red, catalog number 4060T) |
| P49840 | Primary Antibody | Phospho-GSK-3alpha | Human | Rabbit | IgG | 36E9 | Cell Signaling Technology | 9316 | Unconjugated | AB_659836 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human follicular lymphoma FFPE: Phospho-GSK-3 alpha (red, catalog number 9316) |
| Q04206 | Primary Antibody | Phospho-NF-KappaB p65 (Ser536) | Human | Rabbit | IgG | 93H1 | Cell Signaling Technology | 3033 | Unconjugated | AB_331284 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| NA | Primary Antibody | Phospho-SMAD1/5 | Human | Rabbit | IgG | 41D10 | Cell Signaling Technology | 9516 | Unconjugated | AB_491015 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: Phospho-SMAD1/5 (magenta) and DAPI (blue) |
| NA | Primary Antibody | Phospho-SMAD3 | Human | Rabbit | IgG | EP823Y | Abcam | ab52903 | Unconjugated | AB_882596 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: Phospho-SMAD3 (magenta) and DAPI (blue) |
| NA | Primary Antibody | Phospho-TAK1 | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-99340 | Unconjugated | AB_2818273 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: Phospho-TAK1 (magenta) and DAPI (blue) |
| O00329 | Primary Antibody | PI3 Kinase p110 delta | Human | Rabbit | IgG | E2T2N | Cell Signaling Technology | 19591S | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human tonsil FFPE: PI3 Kinase p110 delta (cyan, catalog number 19591S) |
| P01833 | Primary Antibody | PIGR | Human | Rat | IgG1 | 7C1 | Abcam | ab170321 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P01833 | Primary Antibody | PIGR | Human | Rabbit | IgG | Polyclonal | Abcam | ab96196 | Unconjugated | AB_10677612 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreas | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P01833 | Primary Antibody | PIGR | Human | Rat | IgG1 | 7C1 | Abcam | ab170321 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P01833 | Primary Antibody | PIGR | Human | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | PA5-35340 | Unconjugated | AB_2552650 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P17252 | Primary Antibody | PKC alpha | Human | Mouse | IgG1 | H-7 | Santa Cruz Biotechnology | sc-8393 AF680 | AF680 | AB_628142 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: PKC alpha (white, catalog number sc-8393 AF680) and Hoechst (blue, catalog number 40046) |
| P20444 | Primary Antibody | PKC alpha | Mouse | Mouse | IgG1 | H-7 | Santa Cruz Biotechnology | sc-8393 AF680 | AF680 | AB_628142 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | NA |
| NA | Lectin | PNA | Human | NA | NA | NA | Thermo Fisher Scientific | L21409 | AF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Lectin PNA (green, catalog number L21409) and Hoechst (blue, catalog number 40046) |
| NA | Lectin | PNA | Zebrafish | NA | NA | NA | Thermo Fisher Scientific | L21409 | AF488 | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | 1 mg/ml LiBH4 30 minutes + light | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Primary Antibody | PNL-2 | Canine | Mouse | IgG1 | PNL2 | Thermo Fisher Scientific | MSM1-894-P0 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | Canine melanoma skin FFPE: PNL2 (cyan, catalog number MSM1-894-P0 and A21203) and pan-cytokeratin (blue, catalog number 53-9003-80) |
| Q9NP85 | Primary Antibody | Podocin | Human | Rabbit | IgG | JB51-33 | Novus Biologicals | NBP2-75624 | Unconjugated | AB_2892752 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q86YL7 | Primary Antibody | Podoplanin | Human | Mouse | IgG1 | D2-40 | BioLegend | 916601 | Unconjugated | AB_2565182 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: Podoplanin (magenta) and DAPI (blue) |
| Q86YL7 | Primary Antibody | Podoplanin | Human | Rat | IgG2a | NC-08 | BioLegend | 337018 | AF594 | AB_2629590 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9UKI9 | Primary Antibody | POU2F3 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP1-83966 | Unconjugated | AB_11024500 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P02545 | Primary Antibody | Progerin | Human | Mouse | IgG1 | 13A4 | Thermo Fisher Scientific | 39965 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| Q7SY24 | Primary Antibody | Protein kinase C beta | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 12919-1-AP | Unconjugated | AB_2237469 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P42225 | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | Cell Signaling Technology | 9167 | Unconjugated | NA | Stock | IBEX2D Manual | 2% PFA Fixed Frozen | Lung | Infected | Akoya Antibody Diluent | NA | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| P42225 | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | Cell Signaling Technology | 9167 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Liver | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| P42225 | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | Cell Signaling Technology | 9167 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lymph Node | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| P42225 | Primary Antibody | pSTAT1 | Mouse | Rabbit | IgG | 58D6 | Cell Signaling Technology | 9167 | Unconjugated | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | Akoya Antibody Diluent | NA | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| P42229 | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | D47E7 | Cell Signaling Technology | 4322 | Unconjugated | AB_10544692 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P42229 | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | C71E5 | Cell Signaling Technology | 9314 | Unconjugated | AB_2302702 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P42229 | Primary Antibody | pSTAT5 (Tyr694) | Human | Rabbit | IgG | C11C5 | Cell Signaling Technology | 9359 | Unconjugated | AB_823649 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16710 | iF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11037 | AF594 | AB_2534095 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Mouse | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25303 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31572 | AF555 | AB_162543 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11374 | AF790 | AB_2534145 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31572 | AF555 | AB_162543 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: CD3 (red, catalog number 300448), Collagen IV (white, catalog number ab6586 and A-21038), and EpCAM (blue, catalog number 324228) |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-10040 | AF546 | AB_2534016 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25305 | AF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | MilliporeSigma | SAB4600110 | CF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Proteintech | CL594-66467 | CL594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16833 | iF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16690 | iF555 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16686 | iF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16678 | iF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Abcam | ab155728 | AF750 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Vector Laboratories | TI-10000 | Texas Red | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16712 | iF680 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | AAT Bioquest | 16720 | iF750 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21206 | AF488 | AB_2535792 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11009 | AF532 | AB_2534076 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21074 | AF660 | AB_2535735 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21109 | AF680 | AB_2535758 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11034 | AF488 | AB_2576217 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21206 | AF488 | AB_2535792 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11010 | AF546 | AB_2534077 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25303 | AF532 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21206 | AF488 | AB_2535792 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-10043 | AF680 | AB_2534018 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25308 | AF647 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Mouse | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25307 | AF594 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21207 | AF594 | AB_141637 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31573 | AF647 | AB_2536183 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21038 | AF700 | AB_2535709 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Zebrafish | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21245 | AF647 | AB_2535813 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Zenon Labeling Kit | Rabbit IgG (H+L) | Human | Goat | Fab2 | Polyclonal | Thermo Fisher Scientific | Z25302 | AF488 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21039 | AF750 | AB_2535710 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21245 | AF647 | AB_2535813 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21428 | AF555 | AB_2535849 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Zebrafish | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-10042 | AF546 | AB_2534017 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-31572 | AF555 | AB_162543 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11009 | AF532 | AB_2534076 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11009 | AF532 | AB_2534076 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-1118-7432 | NA | 0000-0003-1118-7432 | Mouse lymph node: CD35 (yellow, catalog number 740132) and Collagen IV (blue, catalog number ab19808 and A-11009) |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21207 | AF594 | AB_141637 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-585-150 | AF594 | AB_2340688 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-625-150 | AF680 | AB_2340698 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21096 | AF680 | AB_2535750 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11007 | AF594 | AB_10561522 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21247 | AF647 | AB_141778 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-48269 | AF488 (Plus) | AB_2893137 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-21094 | AF633 | AB_2535749 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | SA5-10031 | DL755 | AB_2556611 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-605-153 | AF647 | AB_2340694 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-605-153 | AF647 | AB_2340694 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-545-150 | AF488 | AB_2340683 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Jackson ImmunoResearch | 712-605-153 | AF647 | AB_2340694 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-48272 | AF647 (Plus) | AB_2893138 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Rat IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-48270 | AF555 (Plus) | AB_2896336 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q6PBW5 | Primary Antibody | Retinal pigment epithelium-specific protein 65kDa | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 17939-1-AP | Unconjugated | AB_2285290 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q6P027 | Primary Antibody | Retinaldehyde binding protein 1 | Zebrafish | Rabbit | IgG | Polyclonal | Proteintech | 15356-1-AP | Unconjugated | AB_2178530 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P35359 | Primary Antibody | Rhodopsin | Zebrafish | Mouse | IgG1 | 1D4 | Santa Cruz Biotechnology | sc-57432 | Unconjugated | AB_785511 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q9HAT0 | Primary Antibody | ROPN1 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP1-89839 | Unconjugated | AB_11013452 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P51449 | Primary Antibody | RORgamma t | Human | Mouse | IgG2a | 6F3.1 | MilliporeSigma | MABF81 | Unconjugated | AB_11205416 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | SARS-NP | Rhesus Macaque | Rabbit | IgG | Polyclonal | Novus Biologicals | NB100-56576 | Unconjugated | NA | Stock | Opal-plex | 10% Formalin for 7 Days | Lung | Infected | Akoya Antibody Diluent | Akoya AR6 | NA | Yes | 0000-0003-0666-4804 | NA | 0000-0003-0666-4804 | NA |
| O09126 | Primary Antibody | SEMA4D | Mouse | Rat | IgG2a | BMA-12 | BioLegend | 147609 | AF594 | AB_2687303 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21102 | AF680 | AB_2535755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD49a (cyan, catalog number 328310) and Fibrinogen (red, catalog number ab118533 and A-21102) |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-11015 | AF488 | AB_2534082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21436 | AF555 | AB_2535857 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21102 | AF680 | AB_2535755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21102 | AF680 | AB_2535755 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | Sheep IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21448 | AF647 | AB_2535865 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q920G3 | Primary Antibody | SiglecF | Mouse | Rat | IgG2a | E50-2440 | BD Biosciences | 552126 | PE | AB_394341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 | NA |
| Q920G3 | Primary Antibody | SiglecF | Mouse | Rat | IgG2a | 1RNM44N | Thermo Fisher Scientific | 56-1702-80 | AF700 | AB_2637126 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q920G3 | Primary Antibody | SiglecF | Mouse | Rat | IgG2a | E50-2440 | BD Biosciences | 552126 | PE | AB_394341 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P97797 | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | BioLegend | 144027 | AF647 | AB_2721300 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P97797 | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | BioLegend | 144024 | AF488 | AB_2650815 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: DEC205 (magenta, catalog number 138204) and SIRPalpha (cyan, catalog number 144024) |
| P97797 | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | BioLegend | 144024 | AF488 | AB_2650815 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lymph node: gp38 (purple, catalog number 127401) and SIRPalpha (cyan, catalog number 144024) |
| P97797 | Primary Antibody | SIRPalpha | Mouse | Rat | IgG1 | P84 | BioLegend | 144027 | AF647 | AB_2721300 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: Cytokeratin (yellow, catalog number 628608) and SIRPalpha (white, catalog number 144027) |
| Q15796 | Primary Antibody | SMAD2 | Human | Mouse | IgG1 | L16D3 | Cell Signaling Technology | 3103 | Unconjugated | AB_490816 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: SMAD2 (magenta) and DAPI (blue) |
| P84022 | Primary Antibody | SMAD3 | Human | Rabbit | IgG | Polyclonal | Abcam | ab28379 | Unconjugated | AB_2192903 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: SMAD3 (magenta) and DAPI (blue) |
| P05023 | Primary Antibody | Sodium Potassium ATPase | Human | Rabbit | IgG | EP1845Y | Abcam | ab197713 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P05023 | Primary Antibody | Sodium Potassium ATPase | Human | Rabbit | IgG | EP1845Y | Abcam | ab198367 | AF647 | AB_2916056 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 | NA |
| Q06945 | Primary Antibody | SOX4 | Human | Mouse | IgG2b | SOX4/2540 | Novus Biologicals | NBP2-79903AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| Q06945 | Primary Antibody | SOX4 | Human | Mouse | IgG2b | CL5634 | Novus Biologicals | NBP2-61420 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| Q15506 | Primary Antibody | SPA17 | Human | Rabbit | IgG | Polyclonal | Proteintech | 13367-1-AP | Unconjugated | AB_2194631 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P09486 | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | R&D Systems | AF941 (Unconjugated) | AF532 | AB_2892754 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P09486 | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | R&D Systems | AF941 (Unconjugated) | AF532 | AB_2892754 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P09486 | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | R&D Systems | AF941 (Unconjugated) | AF532 | AB_2892754 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: SPARC (red, based on catalog number AF941) |
| P09486 | Primary Antibody | SPARC | Human | Goat | IgG | Polyclonal | R&D Systems | AF941 | Unconjugated | AB_355728 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P14719 | Primary Antibody | ST2 (IL-33R) | Mouse | Rat | IgG2a | U29-93 | BD Biosciences | 566311 | PE | AB_2744490 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-1461-0999 | NA | 0000-0002-1461-0999 | NA |
| P18827 | Primary Antibody | Syndecan-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2780 (Unconjugated) | iF594 | AB_2892765 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| O43318 | Primary Antibody | TAK1 | Human | Rabbit | IgG | Polyclonal | Proteintech | 12330-2-AP | Unconjugated | AB_2140101 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: TAK1 (green) and DAPI (blue) |
| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | BioLegend | 118108 | PE | AB_313832 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | TCR gamma delta | Human | Mouse | IgG1 | B1 | BioLegend | 331225 | PE/Dazzle AF594 | AB_2565533 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | TCR gamma delta | Human | Mouse | IgG1 | B1 | BioLegend | 331210 | PE | AB_1089218 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human thymus: TCRgammadelta (magenta, catalog number 331210) and Keratin 5 (green, catalog number based on 905504) |
| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | BioLegend | 118108 | PE | AB_313832 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse thymus: CD44 (blue, based on catalog number 103002) and TCRgd (yellow, catalog number 118108) |
| NA | Primary Antibody | TCR gamma delta | Mouse | Armenian Hamster | IgG | GL3 | BioLegend | 118128 | AF488 | AB_2562771 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | TCR gamma delta | Human | Mouse | IgG1 | B1 | BioLegend | 331210 | PE | AB_1089218 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | TCR Valpha24 | Human | Mouse | IgG1 | 6B11 | BioLegend | 342919 | PE/Dazzle AF594 | AB_2566311 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | TCR Valpha7.2 | Human | Mouse | IgG1 | 3C10 | BioLegend | 351726 | AF647 | AB_2566335 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: HLA-DR (purple, catalog number 307620) and Valpha7.2 (yellow, catalog number 351726) |
| NA | Primary Antibody | TCR Valpha7.2 | Human | Mouse | IgG1 | 3C10 | BioLegend | 351729 | PE/Dazzle AF594 | AB_2566338 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Primary Antibody | TCR Valpha7.2 | Human | Mouse | IgG1 | 3C10 | BioLegend | 351706 | PE | AB_10899577 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9UIF3 | Primary Antibody | TEKT2 | Human | Rabbit | IgG | Polyclonal | Novus Biologicals | NBP1-86955 | Unconjugated | AB_11017402 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P37173 | Primary Antibody | TGFBR2 | Human | Mouse | IgG1 | MM0056-4F14 | Abcam | ab78419 | Unconjugated | AB_1603198 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: TGFBR2 (magenta) and DAPI (blue) |
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | R&D Systems | MAB2365 | Unconjugated | AB_2232900 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rabbit | IgG | Polyclonal | Abcam | ab185703 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | R&D Systems | MAB2365 | Unconjugated | AB_2232900 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | R&D Systems | MAB2365 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q6U7R4 | Primary Antibody | Tim-4 | Mouse | Rat | IgG1 | 21H12 | BD Biosciences | 742773 | BV421 | AB_2741037 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 | NA | 0000-0003-1431-1398 | NA |
| O15393 | Primary Antibody | TMPRSS2 | Human | Rabbit | IgG | EPR3861 | Abcam | ab92323 | Unconjugated | AB_10585592 | Stock | Multiplexed 2D Imaging | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| O15393 | Primary Antibody | TMPRSS2 | Human | Rabbit | IgG | EPR3861 | Abcam | ab92323 (Unconjugated) | AF647 | AB_2892763 | Custom | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q15661 | Primary Antibody | Tryptase | Human | Mouse | IgG1 | AA1 | Abcam | ab2378 | Unconjugated | AB_303023 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | TUJ1 | BioLegend | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: Beta-tubulin 3 (yellow, catalog number 801203) and CD138 (purple, catalog number 356504) |
| Q9ERD7 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | BioLegend | 657402 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Mouse lung: Beta-tubulin 3 (yellow, based on catalog number 657402), CD68 (cyan, catalog number 137017), and CD44 (magenta, catalog number 103018) |
| Q4QRB4 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | TUJ1 | BioLegend | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | NA |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | TUJ1 | BioLegend | 801202 (Unconjugated) | AF532 | AB_2892767 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-3882-457X | NA | 0000-0002-3882-457X | NA |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | TUJ1 | BioLegend | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | AntigenFix (2h, 4 degrees) Fixed Frozen | Kidney | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-7422-3237 | NA | 0000-0002-7422-3237 | NA |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | AA10 | BioLegend | 657412 | BV421 | AB_2632699 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: Tubulin beta-3 (orange, catalog number 657412) and EpCAM (blue, catalog number 324228) |
| Q9ERD7 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | BioLegend | 657412 | BV421 | AB_2632699 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | TUJ1 | BioLegend | 801203 | AF488 | AB_2564757 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | Human retina: Tubulin_beta-3_chain (green, catalog number 801203) and Hoechst (blue, catalog number 40046) |
| Q9ERD7 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | BioLegend | 657402 (Unconjugated) | AF532 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P09936 | Primary Antibody | UCHL1 | Human | Rabbit | IgG | Polyclonal | Proteintech | 14730-1-AP | Unconjugated | AB_2210497 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: UCHL1 (magenta) and DAPI (blue) |
| P07911 | Primary Antibody | Uromodulin | Human | Mouse | IgG2b | 10.32 | Novus Biologicals | NBP1-50431 | FITC | AB_10012622 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | UT014 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL550 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | UT014 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL550 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | UT015 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL490 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | UT015 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL490 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | UT016 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL650 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| NA | Secondary Antibody | UT019 | Human | Rabbit | IgG | Polyclonal | Cell IDx | HI06B-005 | CL594 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| Q9W6A9 | Primary Antibody | UV opsin | Zebrafish | Rabbit | IgG | Polyclonal | Kerafast | EJH013 | Unconjugated | NA | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P33151 | Primary Antibody | VE-Cadherin | Human | Mouse | IgG2a | BV9 | BioLegend | 348512 | AF594 | AB_2563354 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0002-2950-2683 | NA | 0000-0002-2950-2683 | NA |
| P20152 | Primary Antibody | Vimentin | Mouse | Rat | IgG2a | W16220A | BioLegend | 699308 | AF647 | AB_2888890 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0001-5813-2581 | NA | 0000-0001-5813-2581 | NA |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human liver: HLA-DR (cyan, catalog number 307620) and Vimentin (red, catalog number 677807) |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: CD49a (green, catalog number 328310) and Vimentin (blue, catalog number 677804) |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677802 (Unconjugated) | AF532 | AB_2892753 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human lymph node: DC-SIGN (magenta, catalog number 330112) and Vimentin (yellow, based on catalog number 677802) |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Skin | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human skin: CD117 (magenta, catalog number 313203), CD138 (cyan, catalog number 356524), and Vimentin (blue, catalog number 677804) |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human jejunum: Hoechst (blue, catalog number 40046), CD54 (green, catalog number 353114), and Vimentin (magenta, catalog number 677807) |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677802 (Unconjugated) | BL759/780 | NA | Custom | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P20152 | Primary Antibody | Vimentin | Mouse | Rat | IgG2a | W16220A | BioLegend | 699305 | AF488 | AB_2888889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | Mouse tumor: CD11b (yellow, catalog number 101254) and Vimentin (cyan, catalog number 699305) |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677802 (Unconjugated) | BL759/780 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677802 (Unconjugated) | AF532 | AB_2892753 | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human spleen: CD15 (cyan, catalog number 347420 and 715-545-020) and Vimentin (red, based on catalog number 677802), Human spleen: CD4 (cyan, catalog number 300520) and Vimentin (red, based on catalog number 677802) |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 | NA |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | NA |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Automated | FFPE | Kidney | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | Human kidney: Hoechst (blue, catalog number 40046) and Vimentin (magenta, catalog number 677807) |
| P20152 | Primary Antibody | Vimentin | Mouse | Rat | IgG2a | W16220A | BioLegend | 699305 | AF488 | AB_2888889 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | NA |
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677807 | AF647 | AB_2616801 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0002-2692-1225 | NA | 0000-0002-2692-1225 | NA |
| P04275 | Primary Antibody | VWF | Human | Rabbit | IgG | D8L8G | Cell Signaling Technology | 65707 | Unconjugated | AB_2799692 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | Human lung (Idiopathic Pulmonary Fibrosis) FFPE: VWF (magenta) and DAPI (blue) |
| NA | Primary Antibody | ZO-1 | Zebrafish | Mouse | IgG1 | ZO1-1A12 | Thermo Fisher Scientific | 33-9100 | Unconjugated | AB_2533147 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Primary Antibody | Zpr-1 | Turquoise Killifish | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-43 | Unconjugated | AB_10013803 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q6DH07 | Primary Antibody | Zpr-1 | Zebrafish | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-43 | Unconjugated | AB_10013803 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| P35359 | Primary Antibody | Zpr-3 | Zebrafish | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-45 | Unconjugated | AB_10013805 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Primary Antibody | Zpr-3 | African Clawed Frog (Xenopus laevis) | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-45 | Unconjugated | AB_10013805 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| Q58EE9 | Primary Antibody | Zrf-1 | Zebrafish | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-46 | Unconjugated | AB_10013806 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | GFP transgenic line | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
| NA | Primary Antibody | Zrf-1 | Turquoise Killifish | Mouse | IgG1 | Monoclonal | ZIRC | ZDB-ATB-081002-46 | Unconjugated | AB_10013806 | Stock | IBEX2D Manual | 4% PFA Fixed Frozen | Retina | NA | 0.1% Triton-X-100 | pH 6 (10 mM Sodium Citrate) for 20 minutes in a pressure cooker | NA | Yes | 0009-0000-2047-4228 | NA | 0009-0000-2047-4228 | NA |
