| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P84022 | Primary Antibody | SMAD3 | Human | Rabbit | IgG | Polyclonal | Abcam | ab28379 | Unconjugated | AB_2192903 | Stock | IBEX2D Manual | FFPE | Lung | Idiopathic Pulmonary Fibrosis | 0.3% Triton-X-100 | 10 mM citrate buffer (pH 6.0) for 30 minutes at 95C | NA | Yes | 0000-0002-8728-1735 | NA | 0000-0002-8728-1735 | 1 |
Publications
Additional Notes
- This antibody labels the anticipated subcellular compartment (nucleus, cytoplasm). This antibody was detected using a secondary antibody (Abcam, ab150079). We used a secondary only control to test for non-specific binding of the secondary antibody and observed no signal as expected (not shown).
| Human lung (Idiopathic Pulmonary Fibrosis) FFPE: SMAD3 (magenta) and DAPI (blue) |
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