UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P01903 Primary Antibody HLA-DR Human Mouse IgG2a L243 BioLegend 307625 AF700 AB_493770 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 1

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Additional Notes

  1. Clone L243 labels professional antigen presenting cells in fixed frozen tissues prepared from diverse organs. Several conjugates are suitable for IBEX experiments. AF700 conjugate works in non-iterative, single cycle imaging but underperforms in IBEX experiments. Use AF488, iF594, AF647 instead.