| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BD Biosciences | 553032 | PE | AB_394571 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | 1 |
Publications
Additional Notes
- Antibody labels T cells in lung as expected. Clone 53-6.7 has been extensively validated in multiplexed 2D imaging and IBEX2D manual imaging. Works well in Ce3D-IBEX at a dilution of 1:40.