UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P06332 Primary Antibody CD4 Mouse Rat IgG2a RM4-5 Thermo Fisher Scientific 41-0042-82 eF570 AB_2573595 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Pancreatic Ductal Adenocarcinoma Tumor 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0009-0007-0646-7946 NA 0009-0007-0646-7946 1

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Additional Notes

  1. This antibody labels CD4 positive T cells and macrophages in various mouse tissues. In the tumor, the AF647 (BioLegend 100533), AF488 (BD Biosciences 557667) and eF570 (Thermo Fisher Scientific 41-0042-82) conjugates work very well and label CD4+ T cells and macrophages.