| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Mouse | IgG2a | DC28 | R&D Systems | MAB16211-SP | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | LSBio | LS-B3782 | Unconjugated | AB_10689801 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 2 |
| Q9NNX6 | Primary Antibody | DC-SIGN | Human | Rat | IgG2a | h209 | LSBio | LS-B3782 | Unconjugated | AB_10689801 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 3 |
Publications
- A. J. Radtke et al., “A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes”, bioRxiv, 2022, doi: 10.1101/2022.06.03.494716.
Additional Notes
- Evaluated in human lymph node with HLA-DR (clone L243) and shown to label dendritic cells and macrophages. Clone 9E9A8 is preferred for fixed frozen tissues because it is available as a direct conjugate.
- Validated in human follicular lymphoma FFPE samples and shown to label dendritic cells and macrophages; amplified with donkey anti-rat AF647 (Jackson ImmunoResearch 712-605-153).
- Validated in human tonsil FFPE samples and shown to label dendritic cells and macrophage. Works beautifully on Cell DIVE imager with IBEX dye inactivation protocol.