| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P16070 | Primary Antibody | CD44 | Human | Rat | IgG2b | IM7 | BioLegend | 103074 | Spark YG 570 | AB_2892265 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
Publications
Additional Notes
- Clone IM7 labels various cell types and cell states in diverse mouse and human tissues. Several conjugates are suitable for Multiplexed 2D Imaging and IBEX imaging. Antibody yields a great signal; however, the Spark YG570 conjugate does not dye inactivate within 15 minutes of 1 mg/ml LiBH4 treatment. Additional dye inactivation conditions need to be tested. This antibody could be placed in the last cycle or used as a fiducial.