UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 41-4777-82 eF570 AB_2573609 Stock IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256 1
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 41-4777-82 eF570 AB_2573609 Stock IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256 1

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Additional Notes

  1. IBEX2D Manual is performed on tissue biopsies adhered to glass slides. After imaging, coverslips are soaked off and LiBH4 is placed directly onto the tissues. Three exchanges of LiBH4 (15 minute per incubation) are performed to ensure complete removal of signal.