| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P02533 | Primary Antibody | Keratin 14 | Human | Chicken | IgY | Poly9060 | BioLegend | 906004 (Unconjugated) | AF647 | NA | Custom | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
Publications
Additional Notes
- Unconjugated antibody previously validated in skin (Radtke et al., Nature Protocols). Custom conjugate created by BioLegend. Antibody labels epithelium of tonsil based on morphology, spatial pattern, and colocalization with pan-cytokeratin (AE1/AE3) antibody. Dual antigen retrieval yields a robust signal.