UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P08575 Primary Antibody CD45 Human Mouse IgG1 2B11+PD7/26 Novus Biologicals NBP2-34528AF488 AF488 NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0009-0006-9784-2694 NA 0009-0006-9784-2694 1

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Additional Notes

  1. No specific signal seen, only background and autofluorescent RBCs visible.
Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD45 (green, Novus Biologicals cat. no. NBP2-34528AF488)