UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Primary Antibody B220 Mouse Rat IgG2a RA3-6B2 BioLegend 103226 AF647 AB_389330 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA NA NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-1431-1398 [1, 2] NA 0000-0003-1431-1398 1

Publications

  1. A. Gola et al., “Commensal-driven immune zonation of the liver promotes host defence”, Nature, 589(7840):131-136, 2021, doi:10.1038/s41586-020-2977-2.

  2. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. For additional details on how to prepare samples (perfusion, no detergent) see [Gola et al. 2020].