UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q62011 Primary Antibody gp38 Mouse Syrian Hamster IgG 8.1.1 BioLegend 127401 Unconjugated AB_1089186 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1

Publications

  1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Validated by Evelyn Kandov (No ORCID)
Mouse lymph node: gp38 (purple, catalog number 127401) and SIRPalpha (cyan, catalog number 144024)