UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 0.5 mg/ml LiBH4 10 minutes continuous exchange with automated protocol Yes 0000-0003-4379-8967 [2] NA 0000-0003-4379-8967  
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [4, 2] NA 0000-0003-4379-8967  
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [4, 2] NA 0000-0003-4379-8967  
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967  
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 [3] NA 0000-0003-4379-8967  
P11836 Primary Antibody CD20 Human Mouse IgG2a L26 Thermo Fisher Scientific 53-0202-82 AF488 AB_10734358 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [3] NA 0000-0003-4379-8967  

Publications

  1. Q. Xu et al., “Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children”, Nat. Nat Immunol., , doi: 10.1038/s41590-022-01367-z.

  2. A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.

    A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.

  3. A. J. Radtke et al., “A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes”, bioRxiv, 2022, doi: 10.1101/2022.06.03.494716.

  4. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

Human lymph node: CD163 (magenta, catalog number 333620) and CD20 (cyan, catalog number 53-0202-82)
Human spleen: CD20 (purple, catalog number 53-0202-82) and Lumican (cyan, based on catalog number AF2846)