UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Blocking Reagent Fc Block Human Human IgG1 Fc1 BD Biosciences 564220 Unconjugated AB_2869554 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
NA Blocking Reagent Fc Block Mouse Rat IgG2b 2.4G2 BD Biosciences 553141 Unconjugated AB_394656 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 2

Publications

Additional Notes

  1. This reagent is added at a 1:100 dilution to all blocking and labeling buffers for human tissues.
  2. This reagent is added at a 1:100 dilution to all blocking and labeling buffers for mouse tissues. Be careful! This antibody is raised in a rat and cannot be used with anti-rat secondary antibodies.