UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q9BYG3 Primary Antibody Ki-67 Human Rabbit IgG Polyclonal Novus Biologicals NB500-170b Biotin AB_1660247 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1
Q9BYG3 Primary Antibody Ki-67 Human Rabbit IgG Polyclonal Novus Biologicals NB500-170b Biotin AB_1660247 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 2
Q9BYG3 Primary Antibody Ki-67 Human Rabbit IgG Polyclonal Novus Biologicals NB500-170b Biotin AB_1660247 Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 3

Publications

  1. Q. Xu et al., “Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children”, Nat. Nat Immunol., , doi: 10.1038/s41590-022-01367-z.

Additional Notes

  1. Evaluated in human tonsil FFPE samples with CD20 (clone L26) and Hoechst (nuclear marker). Antibody labels germinal centers.
  2. Evaluated in human tonsil FFPE samples with CD20 (clone L26) and Hoechst (nuclear marker). Antibody labels germinal centers with addition of Streptavidin AF750.
  3. Antibody labels dividing cells with addition of Streptavidin AF750.