UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P07766 Primary Antibody CD3 Human Rabbit IgG EP4426 Abcam ab208514 AF555 AB_443425 Stock IBEX2D Manual FFPE Cervix Cervical Cancer 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4184-3867 NA 0000-0003-4184-3867 1
P07766 Primary Antibody CD3 Human Rabbit IgG EP4426 Abcam ab208514 AF555 AB_443425 Stock IBEX2D Manual FFPE Skin NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4184-3867 NA 0000-0003-4184-3867 1

Publications

Additional Notes

  1. Evaluated in human FFPE samples with CD8 and Hoechst (nuclear marker). Antibody yields anticipated staining pattern. Performed using manual IBEX protocol with antibody incubation using the Pelco Biowave microwave.