| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Secondary Antibody | Goat IgG (H+L) | Canine | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21447 | AF647 | AB_2535864 | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | 1 |
Publications
Additional Notes
- Used to detect lumican (R&D Systems, AF2846).
| Canine melanoma skin FFPE: Lumican (blue, catalog number AF2846 and A-21447), a-SMA (magenta, catalog number 41-9760-82), pan-cytokeratin (green, catalog number 53-9003-80), and Hoechst (yellow, catalog number 40046) |
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