| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P16410 | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | Abcam | ab270127 | AF647 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| P16410 | Primary Antibody | CTLA4 | Human | Rabbit | IgG | CAL49 | Abcam | ab270127 | AF647 | NA | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
Publications
Additional Notes
- Labels T cells and colocalizes with CD3, CD4 or CD8, and PD-1 in tonsil. Brighter signal with dual antigen retrieval.
- Antibody labels T follicular helper cells in the germinal center and T cells in the paracortex of human FFPE lymph nodes from follicular lymphoma patients.
| Human FFPE follicular lymphoma lymph node: CTLA-4 (yellow, catalog number ab270127) |
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| Human FFPE follicular lymphoma lymph node: CTLA-4 (yellow, catalog number ab270127 and PD-1 (cyan, catalog number ab275126) |
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