UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103002 (Unconjugated) AF532 AB_2892768 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [2, 1] NA 0000-0003-4379-8967 1
P16070 Primary Antibody CD44 Human Rat IgG2b IM7 BioLegend 103002 (Unconjugated) AF532 AB_2892768 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [2, 1] NA 0000-0003-4379-8967 1
P15379 Primary Antibody CD44 Mouse Rat IgG2b IM7 BioLegend 103002 (Unconjugated) AF532 AB_2892768 Custom IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [2] NA 0000-0003-4379-8967 1

Publications

  1. A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.

    A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.

  2. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Custom conjugation by BioLegend
Human lymph node: CD21 (magenta, based on catalog number 354902) and CD44 (cyan, based on catalog number 103002)
Mouse thymus: CD44 (blue, based on catalog number 103002) and TCRgd (yellow, catalog number 118108)
Human liver: Cytokeratin 7 (green, catalog number 601606), CD44 (blue, based on catalog number 103002) and CD45 (magenta, catalog number 304060)