UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Secondary Antibody Mouse IgG (H+L) Human Donkey IgG Polyclonal Thermo Fisher Scientific A-21203 AF594 AB_141633 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 for more than 120 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1

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Additional Notes

  1. This antibody can be used to evaluate unconjugated mouse primary antibodies in a wide range of tissues. Be careful when using unconjugated primary antibodies as IBEX protocol does not strip antibodies. Place antibodies in first cycle and use direct conjugates in later cycles. AF594 conjugate can be used as a fiducial.