| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q15116 | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | Abcam | ab275126 | AF555 | AB_2728811 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| Q15116 | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | Abcam | ab275126 | AF555 | AB_2728811 | Stock | Multiplexed 2D Imaging | FFPE | Lymph node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
Publications
Additional Notes
- Labels T follicular helper cells in germinal centers and colocalizes with CD3. Brighter signal with dual antigen retrieval.
- Antibody labels T follicular helper cells in the germinal centers of human FFPE lymph nodes and tonsils. Use at a high concentration (1:10) for best results.
| Human FFPE follicular lymphoma lymph node: PD-1 (cyan, catalog number ab275126) |
|---|
 |
| Human FFPE follicular lymphoma lymph node: HLA-DR (red, catalog number NBP2-47670AF488) and PD-1 (cyan, catalog number ab275126) |
|---|
 |
| Human FFPE follicular lymphoma lymph node: CTLA-4 (yellow, catalog number ab270127 and PD-1 (cyan, catalog number ab275126) |
|---|
 |