| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 1 |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | |
| P31996 | Primary Antibody | CD68 | Mouse | Rat | IgG2a | FA-11 | BioLegend | 137017 | BV421 | AB_2562949 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 1 |
Publications
- A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117
Additional Notes
- Validated by Evelyn Kandov (No ORCID)
| Mouse spleen: CD11c (cyan, catalog number 117312) and CD68 (red, catalog number 137017) |
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| Mouse thymus: CD68 (cyan, catalog number 137017) and MHCII (red, catalog number 107622) |
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| Mouse lung: Beta-tubulin 3 (yellow, based on catalog number 657402), CD68 (cyan, catalog number 137017), and CD44 (magenta, catalog number 103018) |
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| Mouse lymph node: F4/80 (cyan, catalog number 123132), CD68 (blue, catalog number 137017) and NK1.1 (magenta, catalog number 108731) |
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