| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P08670 | Primary Antibody | Vimentin | Human | Mouse | IgG2a | O91D3 | BioLegend | 677804 | AF594 | AB_2566179 | Stock | IBEX2D Manual | FFPE | Lung | Infected | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-3697-5726 | NA | 0000-0003-3697-5726 | 1 |
Publications
Additional Notes
- Conjugate requires extended treatment with 1 mg/ml LiBH4 (120 minutes or more) to extinguish signal. Use in the last cycle or as a fiducial for IBEX experiments. Provides a good signal in highly autofluorescent FFPE lung tissue infected with Mycobacterium avium.