UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
P13569	Primary Antibody	CFTR	Human	Mouse	IgM	CF3	Thermo Fisher Scientific	MA1-935	Unconjugated	AB_2081230	Stock	Multiplexed 2D Imaging	1:4 Cytofix/Cytoperm Fixed Frozen	Pancreas	NA	0.3% Triton-X-100	NA	NA	No	0000-0002-2950-2683	NA	0000-0002-2950-2683	1
P13569	Primary Antibody	CFTR	Human	Mouse	IgM	CF3	Thermo Fisher Scientific	MA1-935	Unconjugated	AB_2081230	Stock	Multiplexed 2D Imaging	FFPE	Tonsil	NA	0.3% Triton-X-100	pH 6 for 30 minutes ER1 (AR9961) using the Leica Bond	NA	Yes	0000-0002-2950-2683	NA	0000-0002-2950-2683	2
P13569	Primary Antibody	CFTR	Human	Mouse	IgG1	M3A7	Thermo Fisher Scientific	MA5-11768	Unconjugated	AB_10983660	Stock	Multiplexed 2D Imaging	1:4 Cytofix/Cytoperm Fixed Frozen	Pancreas	NA	0.3% Triton-X-100	NA	NA	No	0000-0002-2950-2683	NA	0000-0002-2950-2683	3

Publications

Additional Notes

1. Evaluated in pancreas as a positive control. Low signal and high background observed on endocrine cells. Inconclusive results.
2. Evaluated in FFPE tonsil and appears to stain all B cells. Yields similar pattern to vendor sheet.
3. Evaluated in pancreas as a positive control. Antibody yields inconclusive results and doesn’t label anticipated cell types.