UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P06332 Primary Antibody CD4 Mouse Rat IgG2b GK1.5 BD Biosciences 568161 RY586 AB_3684090 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 1X BD PermWash Buffer NA NA Yes 0000-0002-6863-1461 NA 0000-0002-6863-1461 1
P06332 Primary Antibody CD4 Mouse Rat IgG2b GK1.5 BD Biosciences 568161 RY586 AB_3684090 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 1X BD PermWash Buffer NA 1 mg/ml LiBH4 15 minutes Yes 0000-0002-6863-1461 NA 0000-0002-6863-1461 2
P06332 Primary Antibody CD4 Mouse Rat IgG2b GK1.5 BD Biosciences 568161 RY586 AB_3684090 Stock Ce3D 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 1X BD PermWash Buffer NA NA Yes 0000-0002-6863-1461 NA 0000-0002-6863-1461 3

Publications

Additional Notes

  1. Immunized draining lymph node. Stain was in 1X Perm Wash Buffer. Antibody concentration ~1/150, stained overnight at 4C.
  2. Immunized draining lymph node. Stain was in 1X Perm Wash Buffer. Antibody concentration ~1/150, stained overnight at 4C. Dye inactivation was performed using 1mg/mL LiBH4 for 15 minutes.
  3. Immunized draining lymph node. Stain was in 1X Perm Wash Buffer. Antibody concentration 1/50, stained 3-4 days at RT on shaker. Did not post-fix. Cleared in Ce3D overnight.
Immunized mouse lymph node
Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) and identical Imaris channel settings to the pre-inactivation image to determine susceptibility to signal loss with dye inactivation.
Immunized mouse lymph node: dye-inactivated section imaged using pre-inactivation microscope settings (laser power, gain) but with signal maximally increased in Imaris to display the completeness of signal loss with dye inactivation.
Immunized mouse lymph node: 2D slice at the edge of a ~250um-thick 3D image
Immunized mouse lymph node: 2D slice at the centre of a ~250um-thick 3D image