| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P05362 | Primary Antibody | CD54 | Human | Mouse | IgG1 | HA58 | BioLegend | 353125 | AF700 | AB_2810560 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
Publications
Additional Notes
- Clone HA58 is expressed on a variety of cell types (immune and stroma). Antibody yielded anticipated spatial pattern and colocalized with vimentin, alpha smooth muscle actin, and CD45. AF647 conjugate yields a better signal than AF700. This clone may be sensitive to cycling order and/or LiBH4, but needs to be evaluated more carefully.