UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
P51884	Primary Antibody	Lumican	Human	Rabbit	IgG	EPR8898(2)	Abcam	Ab252012	PE	NA	Stock	Multiplexed 2D Imaging	FFPE	Lymph Node	Follicular Lymphoma	0.3% Triton-X-100	pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond	NA	No	0000-0003-4379-8967	NA	0000-0003-4379-8967	1

Publications

Additional Notes

1. Beautiful staining pattern observed; however, antibody (Ab252012) appears to label B cells in the follicle (concentrated circle in center of image) and mesenchymal cells (based on appearance and location) outside of the follicle. Left image depicts lumican antibody (red, Ab252012) in follicular lymphoma FFPE sample prepared with dual antigen retrieval (pH 6 and 9). Right image demonstrates that Lumican PE (Ab252012, red) does not colocalize with a well characterized polyclonal antibody (Lumican R&D Systems AF2846, cyan) and secondary antibody (Thermo Fisher Scientific A-21084). Similar results, a follicular staining pattern, were observed in human FFPE tonsil samples.

   Human follicular lymphoma FFPE : Lumican PE (red, ab252012 ) pH 6 and 9	Human follicular lymphoma FFPE : Lumican (cyan, AF2846) and Lumican PE (red, ab252012) pH 6 and 9