UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | Novus Biologicals | NBP2-34522IR | DL755 | NA | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
P62736 | Primary Antibody | Alpha smooth muscle actin | Human | Mouse | IgG2a | 1A4/asm-1 | Novus Biologicals | NBP2-34522IR | DL755 | NA | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
Publications
Additional Notes
- Labels stromal and smooth muscle cells. Beautiful signal with dual antigen retrieval and DL755 conjugate.
- Labels stromal and smooth muscle cells. Beautiful signal with dual antigen retrieval and DL755 conjugate. Imaged using dye inactivation protocol on Cell DIVE platform.