| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Primary Antibody | B220 | Mouse | Rat | IgG2a | RA3-6B2 | Thermo Fisher Scientific | 48-0452-82 | eF450 | AB_1548761 | Stock | Ce3D-IBEX | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 1:10 BD PermWash | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0003-9817-7874 | NA | 0009-0003-9817-7874 | 1 |
Publications
Additional Notes
- Antibody labels B cells in lymph node as expected. Clone RA3-6B2 has been extensively validated in multiplexed 2D imaging and IBEX2D manual imaging. This antibody is recommended for 3D tissue volumes. Recommend 1:50 dilution.