| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Lectin | Isolectin GS-IB4 | Human | NA | NA | NA | Thermo Fisher Scientific | I21412 | AF568 | NA | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Retina | NA | 0.1% Saponin | NA | NA | Yes | 0000-0003-2088-8310 | NA | 0000-0003-2088-8310 | 1 |
Publications
Additional Notes
- Include with primary and secondary antibodies. No special buffers needed. Use at a dilution of 1:500. Isolectin GS-IB4 is a 114,000-dalton glycoprotein that is part of a family of five tetrameric type I isolectins (IA4, IA3B, IA2B2, IAB3, and IB4) isolated from the seeds of the tropical African legume Griffonia simplicifolia. The A subunit prefers N-acetyl-D-galactosamine end groups while the B subunit is selective for terminal a-D-galactosyl residues. AF568 conjugate does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment.