UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q99JB6 Primary Antibody FOXP3 Mouse Rat IgG2a FJK-16s Thermo Fisher Scientific 53-5773-82 AF488 AB_763537 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  
Q9BZS1 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 53-4777-41 AF488 AB_10852836 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1

Publications

  1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Clone 236A/E7 works in diverse tissues to label T regulatory cells. Several conjugates are suitable for IBEX imaging. Signal is dim and a high concentration of antibody is needed. eF570 yields the best results.