| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Lymph Node | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | 1 |
| P32302 | Primary Antibody | CD185 (CXCR5) | Human | Rat | IgG2b | RF8B2 | BD Biosciences | 552032 | Unconjugated | AB_394324 | Stock | IBEX2D Manual | FFPE | Tonsil | Metastatic Ovarian Cancer | 0.3% Triton-X-100 | pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) | NA | Yes | 0000-0001-9561-4256 | NA | 0000-0001-9561-4256 | 1 |
Publications
Additional Notes
- IBEX2D Manual is performed on tissue biopsies adhered to glass slides. After imaging, coverslips are soaked off and LiBH4 is placed directly onto the tissues. Visualized using a goat anti-rat IgG AF647 secondary antibody (Thermo Fisher Scientific A-21247).