UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P22083 Primary Antibody CD15 Human Mouse IgM MMA BD Biosciences 347420 Unconjugated AB_400298 Stock IBEX2D Automated FFPE Kidney NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  
P22083 Primary Antibody CD15 Human Mouse IgM MMA BD Biosciences 347420 Unconjugated AB_400298 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Spleen NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 [2, 1] NA 0000-0003-4379-8967  
P22083 Primary Antibody CD15 Human Mouse IgM MMA BioLegend 394702 Unconjugated NA Stock Cell DIVE-IBEX FFPE Lung Cancer 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1

Publications

  1. A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.

    A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.

  2. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. This antibody labels granulocytes. Works very well on the Cell DIVE imager with IBEX dye inactivation protocol.
Human spleen: CD15 (cyan, catalog number 347420 and 715-545-020) and Vimentin (red, based on catalog number 677802)