| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 1 |
| Q99JB6 | Primary Antibody | FOXP3 | Mouse | Rat | IgG2a | FJK-16s | Thermo Fisher Scientific | 50-5773-82 | eF660 | AB_11220082 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | |
| Q9BZS1 | Primary Antibody | FOXP3 | Human | Mouse | IgG1 | 236A/E7 | Thermo Fisher Scientific | 50-4777-42 | eF660 | AB_2574219 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
Publications
- A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117
Additional Notes
- Validated by Evelyn Kandov (No ORCID)
- Clone 236A/E7 works in diverse tissues to label T regulatory cells. Several conjugates are suitable for IBEX imaging. Signal is dim and a high concentration of antibody is needed. eF570 yields the best results.