Summary of fluorescent probes tested by the IBEX Imaging Community. Inactivation conditions are method specific.
Results are reported for the original IBEX2D Manual method that uses 1 mg/ml of LiBH4. The time, concentration of LiBH4, and method (continuous exchange, bleaching in the presence of light) may vary by IBEX configuration or user.
| Fluorescent Probe | Excitation Max (nm) | Emission Max (nm) | Signal Inactivation Conditions IBEX2D Manual |
|---|---|---|---|
| Hoechst | 350 | 461 | Does not bleach |
| VioGreen | 388 | 520 | 1 mg/ml LiBH4 15 minutes |
| VioBlue | 400 | 452 | 1 mg/ml LiBH4 15 minutes |
| Spark Violet 538 | 400 | 538 | 1 mg/ml LiBH4 15 minutes |
| StarBright Violet 670 | 401 | 667 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| StarBright Violet 710 | 402 | 713 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| BV421 | 405 | 421 | 1 mg/ml LiBH4 15 minutes + Light |
| eF450 | 405 | 450 | 1 mg/ml LiBH4 15 minutes |
| BV510 | 405 | 510 | 1 mg/ml LiBH4 15 minutes + Light |
| BV570 | 405 | 570 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| Pacific Blue | 410 | 455 | 1 mg/ml LiBH4 15 minutes |
| Cyan Fluorescent Protein (CFP) | 435 | 485 | Does not bleach |
| StarBright Blue 700 | 473 | 703 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| PerCP-Vio 700 | 482 | 704 | Not tested |
| Green Fluorescent Protein (GFP) | 488 | 510 | Does not bleach |
| iF488 | 488 | 530 | 1 mg/ml LiBH4 15 minutes |
| AF488 | 490 | 525 | 1 mg/ml LiBH4 15 minutes |
| AF488 (Plus) | 490 | 525 | 1 mg/ml LiBH4 15 minutes |
| FITC | 490 | 525 | 1 mg/ml LiBH4 30 minutes |
| CL490 | 491 | 515 | 1 mg/ml LiBH4 15 minutes |
| CoraLite Plus 488 | 493 | 522 | 1 mg/ml LiBH4 15 minutes |
| Spark Blue 574 | 506 | 574 | 1 mg/ml LiBH4 15 minutes |
| iF514 | 511 | 527 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| Yellow Fluorescent Protein (YFP) | 513 | 527 | Does not bleach |
| JOJO-1 | 530 | 544 | Does not bleach |
| AF532 | 532 | 554 | 1 mg/ml LiBH4 15 minutes |
| iF532 | 537 | 560 | 1 mg/ml LiBH4 15 minutes |
| CL550 | 550 | 575 | 1 mg/ml LiBH4 15 minutes |
| Spark YG 570 | 555 | 570 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| AF555 | 555 | 580 | 1 mg/ml LiBH4 15 minutes |
| AF555 (Plus) | 555 | 580 | 1 mg/ml LiBH4 15 minutes |
| Red Fluorescent Protein (RFP) | 555 | 584 | Does not bleach |
| eF570 | 556 | 569 | 1 mg/ml LiBH4 15 minutes |
| AF546 | 556 | 573 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| iF555 | 557 | 570 | 1 mg/ml LiBH4 15 minutes |
| PE | 565 | 578 | 1 mg/ml LiBH4 15 minutes |
| RY586 | 565 | 586 | Not tested |
| PE/iF594 | 565 | 606 | 1 mg/ml LiBH4 15 minutes |
| PE/Dazzle AF594 | 565 | 610 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| PE-Vio 615 | 565 | 619 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| PE-Vio 770 | 565 | 775 | 1 mg/ml LiBH4 15 minutes |
| AF568 | 578 | 603 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| iF594 | 588 | 604 | 1 mg/ml LiBH4 15 minutes |
| AF594 | 590 | 617 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment. |
| CL594 | 593 | 614 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| CF594 | 593 | 615 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| eF615 | 595 | 615 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| Texas Red | 596 | 615 | Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment |
| AF633 | 631 | 650 | 1 mg/ml LiBH4 15 minutes |
| eF660 | 633 | 669 | 1 mg/ml LiBH4 15 minutes |
| AF647 | 650 | 665 | 1 mg/ml LiBH4 15 minutes |
| AF647 (Plus) | 650 | 665 | 1 mg/ml LiBH4 15 minutes |
| APC-Vio 770 | 652 | 775 | Not tested |
| CL650 | 655 | 676 | 1 mg/ml LiBH4 15 minutes |
| iF647 | 656 | 670 | 1 mg/ml LiBH4 15 minutes |
| AF660 | 662 | 690 | 1 mg/ml LiBH4 15 minutes |
| AF680 | 679 | 702 | 1 mg/ml LiBH4 15 minutes |
| iF680 | 684 | 701 | 1 mg/ml LiBH4 15 minutes |
| Spark Red 718 | 687 | 718 | 1 mg/ml LiBH4 15 minutes |
| Vio Bright R720 | 695 | 720 | 1 mg/ml LiBH4 15 minutes |
| AF700 | 702 | 723 | 1 mg/ml LiBH4 15 minutes |
| AF750 | 749 | 775 | 1 mg/ml LiBH4 15 minutes |
| iF750 | 757 | 779 | 1 mg/ml LiBH4 15 minutes |
| BL759/780 | 759 | 780 | 1 mg/ml LiBH4 15 minutes |
| DL755 | 776 | 754 | 1 mg/ml LiBH4 15 minutes |
| AF790 | 784 | 814 | 1 mg/ml LiBH4 15 minutes |
| AF800 (Plus) | 786 | 790 | 1 mg/ml LiBH4 15 minutes |