Summary of fluorescent probes tested by the IBEX Imaging Community. Inactivation conditions are method specific.

Results are reported for the original IBEX2D Manual method that uses 1 mg/ml of LiBH4. The time, concentration of LiBH4, and method (continuous exchange, bleaching in the presence of light) may vary by IBEX configuration or user.

Fluorescent Probe Excitation Max (nm) Emission Max (nm) Signal Inactivation Conditions IBEX2D Manual
Hoechst 350 461 Does not bleach
VioGreen 388 520 1 mg/ml LiBH4 15 minutes
VioBlue 400 452 1 mg/ml LiBH4 15 minutes
Spark Violet 538 400 538 1 mg/ml LiBH4 15 minutes
StarBright Violet 670 401 667 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
StarBright Violet 710 402 713 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
BV421 405 421 1 mg/ml LiBH4 15 minutes + Light
eF450 405 450 1 mg/ml LiBH4 15 minutes
BV510 405 510 1 mg/ml LiBH4 15 minutes + Light
BV570 405 570 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
Pacific Blue 410 455 1 mg/ml LiBH4 15 minutes
Cyan Fluorescent Protein (CFP) 435 485 Does not bleach
StarBright Blue 700 473 703 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
PerCP-Vio 700 482 704 Not tested
Green Fluorescent Protein (GFP) 488 510 Does not bleach
iF488 488 530 1 mg/ml LiBH4 15 minutes
AF488 490 525 1 mg/ml LiBH4 15 minutes
AF488 (Plus) 490 525 1 mg/ml LiBH4 15 minutes
FITC 490 525 1 mg/ml LiBH4 30 minutes
CL490 491 515 1 mg/ml LiBH4 15 minutes
CoraLite Plus 488 493 522 1 mg/ml LiBH4 15 minutes
Spark Blue 574 506 574 1 mg/ml LiBH4 15 minutes
iF514 511 527 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
Yellow Fluorescent Protein (YFP) 513 527 Does not bleach
JOJO-1 530 544 Does not bleach
AF532 532 554 1 mg/ml LiBH4 15 minutes
iF532 537 560 1 mg/ml LiBH4 15 minutes
CL550 550 575 1 mg/ml LiBH4 15 minutes
Spark YG 570 555 570 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
AF555 555 580 1 mg/ml LiBH4 15 minutes
AF555 (Plus) 555 580 1 mg/ml LiBH4 15 minutes
Red Fluorescent Protein (RFP) 555 584 Does not bleach
eF570 556 569 1 mg/ml LiBH4 15 minutes
AF546 556 573 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
iF555 557 570 1 mg/ml LiBH4 15 minutes
PE 565 578 1 mg/ml LiBH4 15 minutes
RY586 565 586 Not tested
PE/iF594 565 606 1 mg/ml LiBH4 15 minutes
PE/Dazzle AF594 565 610 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
PE-Vio 615 565 619 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
PE-Vio 770 565 775 1 mg/ml LiBH4 15 minutes
AF568 578 603 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
iF594 588 604 1 mg/ml LiBH4 15 minutes
AF594 590 617 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment.
CL594 593 614 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
CF594 593 615 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
eF615 595 615 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
Texas Red 596 615 Does not bleach within 15 minutes of 1 mg/ml LiBH4 treatment
AF633 631 650 1 mg/ml LiBH4 15 minutes
eF660 633 669 1 mg/ml LiBH4 15 minutes
AF647 650 665 1 mg/ml LiBH4 15 minutes
AF647 (Plus) 650 665 1 mg/ml LiBH4 15 minutes
APC-Vio 770 652 775 Not tested
CL650 655 676 1 mg/ml LiBH4 15 minutes
iF647 656 670 1 mg/ml LiBH4 15 minutes
AF660 662 690 1 mg/ml LiBH4 15 minutes
AF680 679 702 1 mg/ml LiBH4 15 minutes
iF680 684 701 1 mg/ml LiBH4 15 minutes
Spark Red 718 687 718 1 mg/ml LiBH4 15 minutes
Vio Bright R720 695 720 1 mg/ml LiBH4 15 minutes
AF700 702 723 1 mg/ml LiBH4 15 minutes
AF750 749 775 1 mg/ml LiBH4 15 minutes
iF750 757 779 1 mg/ml LiBH4 15 minutes
BL759/780 759 780 1 mg/ml LiBH4 15 minutes
DL755 776 754 1 mg/ml LiBH4 15 minutes
AF790 784 814 1 mg/ml LiBH4 15 minutes
AF800 (Plus) 786 790 1 mg/ml LiBH4 15 minutes