UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Q64525 | Primary Antibody | Histone H2B | Mouse | Rat | IgG2a | W17025A | BioLegend | 606301 | Unconjugated | AB_2728465 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | No | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | 1 |
Publications
Additional Notes
- Evaluated in mouse lymph node with Hoechst 33342 staining. Used goat anti-rat IgG AF594 (1:250 dilution) as a secondary. Antibody does not label nuclei.