UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q64525 Primary Antibody Histone H2B Mouse Rat IgG2a W17025A BioLegend 606301 Unconjugated AB_2728465 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0002-5187-810X NA 0000-0002-5187-810X 1

Publications

Additional Notes

  1. Evaluated in mouse lymph node with Hoechst 33342 staining. Used goat anti-rat IgG AF594 (1:250 dilution) as a secondary. Antibody does not label nuclei.