UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [3, 1] NA 0000-0003-4379-8967  
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [3, 1] NA 0000-0003-4379-8967  
P62737 Primary Antibody Alpha smooth muscle actin Mouse Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [3] NA 0000-0003-4379-8967  
P62737 Primary Antibody Alpha smooth muscle actin Mouse Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [3] NA 0000-0003-4379-8967 1
P62737 Primary Antibody Alpha smooth muscle actin Mouse Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [3] NA 0000-0003-4379-8967  
P62736 Primary Antibody Alpha smooth muscle actin Human Mouse IgG2a 1A4 Thermo Fisher Scientific 53-9760-82 AF488 AB_2574461 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node Follicular Lymphoma 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [2] NA 0000-0003-4379-8967  

Publications

  1. A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.

    A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.

  2. A. J. Radtke et al., “A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes”, bioRxiv, 2022, doi: 10.1101/2022.06.03.494716.

  3. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Validated by Evelyn Kandov (No ORCID)
Human lymph node: Alpha smooth muscle actin (red, catalog number 53-9760-82)