UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
Q8BHC0	Primary Antibody	LYVE-1	Mouse	Rat	IgG1	ALY7	Thermo Fisher Scientific	41-0443-82	eF570	AB_2573596	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lung	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
Q8BHC0	Primary Antibody	LYVE-1	Mouse	Rat	IgG1	ALY7	Thermo Fisher Scientific	41-0443-82	eF570	AB_2573596	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Small Intestine	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
Q8BHC0	Primary Antibody	LYVE-1	Mouse	Rat	IgG1	ALY7	Thermo Fisher Scientific	41-0443-82	eF570	AB_2573596	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lymph Node	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967

Publications

1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

1. Validated by Evelyn Kandov (No ORCID)