| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q9ERD7 | Primary Antibody | Tubulin beta-3 chain | Mouse | Mouse | IgG2a | AA10 | BioLegend | 657412 | BV421 | AB_2632699 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | |
| Q13509 | Primary Antibody | Tubulin beta-3 chain | Human | Mouse | IgG2a | AA10 | BioLegend | 657412 | BV421 | AB_2632699 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
Publications
- A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117
Additional Notes
- Antibody labels nerves in jejunum but high autofluorescence observed with BV421 conjugates in this tissue. Background subtraction with channel arithmetics is recommended.
| Human jejunum: Tubulin beta-3 (orange, catalog number 657412) and EpCAM (blue, catalog number 324228) |
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