UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Primary Antibody HLA-DR Canine Mouse IgG1 TAL 1B5 Abcam ab176408 (Unconjugated) iF594 NA Custom Multiplexed 2D Imaging FFPE Lymph Node NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0001-5088-7808 NA 0000-0001-5088-7808 1

Publications

Additional Notes

  1. Autofluorescence from red blood cells present in this channel. Great signal to noise overall.
Canine lymph node FFPE: LYVE1 (magenta, catalog number AF2089 and A21203) and HLA-DR (blue, catalog number ab176408)