| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 [2, 1] | NA | 0000-0003-4379-8967 | |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | IBEX2D Automated | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | |
| Q9Y5Y7 | Primary Antibody | LYVE-1 | Human | Goat | IgG | Polyclonal | R&D Systems | AF2089 | Unconjugated | AB_355144 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1,2 |
Publications
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A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.
A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.
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A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117
Additional Notes
- Antibody labels lymphatic endothelial cells and macrophages as expected.
- Antibody labels lymphatic endothelial cells and vessels in FFPE tissues. Excellent antibody used throughout many studies. Used at a dilution of 1:100 with an anti-goat AF555 secondary antibody (Thermo Fisher Scientific catalog number A-21432).
| Human follicular lymphoma FFPE: LYVE-1 (yellow, catalog number AF2089) and Hoechst (blue, catalog number 40046) |
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