UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
P63158 | Primary Antibody | HMGB-1 | Mouse | Mouse | IgG2 | 3E8 | BioLegend | 651406 | AF594 | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0002-5187-810X | NA | 0000-0002-5187-810X | 1 |
Publications
Additional Notes
- Evaluated in mouse lymph node with Hoechst 33342 staining. Antibody signal colocalized with Hoechst staining.