| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | 0000-0003-4184-3867 | NA | 0000-0003-4184-3867 | 1 |
Publications
Additional Notes
- Evaluated in human FFPE tissues with Hoechst (nuclear marker). Antibody yields anticipated staining pattern. Performed using manual IBEX protocol with antibody incubation using the Pelco Biowave microwave.