| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P62737 | Primary Antibody | Alpha smooth muscle actin | Mouse | Mouse | IgG2a | 1A4 | Thermo Fisher Scientific | 53-9760-82 | AF488 | AB_2574461 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Pancreatic Ductal Adenocarcinoma | Tumor | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | 0009-0007-0646-7946 | NA | 0009-0007-0646-7946 | 1 |
Publications
Additional Notes
- This alpha smooth muscle actin antibody labels cytoskeleton proteins in various mouse tissues. In the tumor, the AF488 conjugate (53-9760-82) works very well and labels actin family of proteins, smooth muscle cells, and cancer associated fibroblasts.