UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
NA Primary Antibody TCR gamma delta Mouse Armenian Hamster IgG GL3 BioLegend 118108 PE AB_313832 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [2] NA 0000-0003-4379-8967 1
NA Primary Antibody TCR gamma delta Human Mouse IgG1 B1 BioLegend 331210 PE AB_1089218 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [1, 2] NA 0000-0003-4379-8967  
NA Primary Antibody TCR gamma delta Mouse Armenian Hamster IgG GL3 BioLegend 118108 PE AB_313832 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 [1, 2] NA 0000-0003-4379-8967  
NA Primary Antibody TCR gamma delta Human Mouse IgG1 B1 BioLegend 331210 PE AB_1089218 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 2

Publications

  1. A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.

    A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.

  2. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Validated by Evelyn Kandov (No ORCID)
Mouse thymus: CD44 (blue, based on catalog number 103002) and TCRgd (yellow, catalog number 118108)
  1. Referenced in N. Yayon et al., “A spatial human thymus cell atlas mapped to a continuous tissue axis”, bioRxiv, 2024, doi: 10.1101/2023.10.25.562925.
Human thymus: TCRgammadelta (magenta, catalog number 331210) and Keratin 5 (green, catalog number based on 905504)