| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P16422 | Primary Antibody | EpCAM | Human | Mouse | IgG2b | 9C4 | BioLegend | 324235 | BV510 | AB_2632934 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Jejunum | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| Q99JW5 | Primary Antibody | EpCAM | Mouse | Rat | IgG2a | G8.8 | BD Biosciences | 747748 | BV510 | AB_2872217 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | No | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 2 |
Publications
- A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117
Additional Notes
- Antibody was tested by standard, single cycle multiplexed tissue imaging and did not provide reliable staining. Other conjugates are recommended (AF488, AF594 (fiducial), AF647). Additionally, BV510 dyes require bleaching in the presence of light. Characterized by Evelyn Kandov (No ORCID).
- Validated by Evelyn Kandov (No ORCID); however, other conjugates are preferred because they do not require bleaching in the presence of LiBH4 and light (AF488, AF594 (fiducial), AF647).