| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Secondary Antibody | Mouse IgG1 | Human | Alpaca | IgG1 | CTK0103/CTK0104 | Thermo Fisher Scientific | SA5-10331 | AF568 | AB_2868378 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
Publications
Additional Notes
- This secondary antibody can be combined with other mouse IgG isotype classes (IgG2a, IgG2b) without cross-reactivity. This conjugate does not dye inactivate within 15 minutes of 1 mg/ml LiBH4 treatment. Additional dye inactivation conditions need to be tested. This antibody may work best in other conjugates for Cell-DIVE IBEX and IBEX experiments.