UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
P08670	Primary Antibody	Vimentin	Human	Mouse	IgG2a	O91D3	BioLegend	677802 (Unconjugated)	AF532	AB_2892753	Custom	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Spleen	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [2, 1]	NA	0000-0003-4379-8967	1
P08670	Primary Antibody	Vimentin	Human	Mouse	IgG2a	O91D3	BioLegend	677802 (Unconjugated)	AF532	AB_2892753	Custom	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lymph Node	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [2, 1]	NA	0000-0003-4379-8967	1

Publications

1. A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.

   A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.

2. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

1. Custom conjugation by BioLegend

Human spleen: CD4 (cyan, catalog number 300520) and Vimentin (red, based on catalog number 677802)

Human lymph node: DC-SIGN (magenta, catalog number 330112) and Vimentin (yellow, based on catalog number 677802)

Human spleen: CD15 (cyan, catalog number 347420 and 715-545-020) and Vimentin (red, based on catalog number 677802)