UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P04234 Primary Antibody CD3 Human Mouse IgG1 UCHT1 BioLegend 300487 Spark Blue 574 AB_2904329 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1

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Additional Notes

  1. Clone UCHT1 labels T cells faithfully. This conjugate is very bright, labels T cells, but cannot be combined with Hoechst. Conjugate inactivates with just 15 minutes of 1 mg/ml of LiBH4.