UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P33681 Primary Antibody CD80 Human Mouse IgG2b 62N3G8 Novus Biologicals NBP2-25255AF488 AF488 NA Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA No 0000-0002-3882-457X NA 0000-0002-3882-457X 1

Publications

Additional Notes

  1. Evaluated in human lymph node with HLA-DR (clone L243) to label professional antigen presenting cells. Antibody did not yield expected staining pattern. CD80 may be sensitive to detergents in the blocking and staining buffers. This needs to be carefully examined.